Isolated carp antiviral protein pdcd6ip and its antiviral activity
An anti-virus and carp spring viremia technology is applied in the direction of antiviral agents, peptide/protein components, and resistance to vector-borne diseases, which can solve the problems of lack of effective control of carp spring viremia drugs and methods, and reduce the infectious effect
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[0033] The following describes several preferred embodiments of the present invention with reference to the accompanying drawings, so as to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.
[0034] (1) RNA extraction from carp
[0035] The total RNA of the samples to be tested was extracted by the classic Trizol method. Cut carp tissue pieces with ophthalmic scissors, put them directly into a mortar, add a small amount of liquid nitrogen, and grind them quickly. ) for 10 minutes, and then centrifuged at 12000×g for 5 minutes at 4°C.
[0036] Add 200 μL of chloroform to the supernatant, vortex and mix well, place at room temperature for 15 min, centrifuge at 12000×g for 15 min at 4 °C.
[0037] Take the upper aqueous phase to another new centrifuge tube, add 500 μL of isopropanol, place...
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