Isolated carp antiviral protein pdcd6ip and its antiviral activity

An anti-virus and carp spring viremia technology is applied in the direction of antiviral agents, peptide/protein components, and resistance to vector-borne diseases, which can solve the problems of lack of effective control of carp spring viremia drugs and methods, and reduce the infectious effect

Active Publication Date: 2022-05-20
上海市水产研究所(上海市水产技术推广站)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the internationally accepted effective measures to control the disease are to monitor carp spring viremia, detect it early and carry out isolation or culling, and there is still a lack of drugs and methods to effectively control carp spring viremia

Method used

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  • Isolated carp antiviral protein pdcd6ip and its antiviral activity
  • Isolated carp antiviral protein pdcd6ip and its antiviral activity
  • Isolated carp antiviral protein pdcd6ip and its antiviral activity

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Embodiment Construction

[0033] The following describes several preferred embodiments of the present invention with reference to the accompanying drawings, so as to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.

[0034] (1) RNA extraction from carp

[0035] The total RNA of the samples to be tested was extracted by the classic Trizol method. Cut carp tissue pieces with ophthalmic scissors, put them directly into a mortar, add a small amount of liquid nitrogen, and grind them quickly. ) for 10 minutes, and then centrifuged at 12000×g for 5 minutes at 4°C.

[0036] Add 200 μL of chloroform to the supernatant, vortex and mix well, place at room temperature for 15 min, centrifuge at 12000×g for 15 min at 4 °C.

[0037] Take the upper aqueous phase to another new centrifuge tube, add 500 μL of isopropanol, place...

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Abstract

The invention relates to the technical field of genetic engineering of freshwater fish, and discloses an isolated carp antiviral protein Pdcd6ip and its antiviral activity. The nucleotide sequence of the gene of the carp antiviral protein Pdcd6ip is 1-2610bp in SEQ ID NO:1 The sequence shown, the encoded amino acid sequence is the sequence shown in SEQ ID NO:2. Tests show that the expression of the Pdcd6ip protein cloned by the present invention can affect the proliferation of carp spring viremia virus (SVCV), and the Pdcd6ip protein plays an important role in the process of cell defense against SVCV infection, and has a low impact on the cell activity of ordinary cells . The gene or protein of the invention provides a new target for preparing anti-SVCV medicines.

Description

technical field [0001] The invention relates to the technical field of freshwater fish genetic engineering, in particular to an isolated carp antiviral protein Pdcd6ip and its antiviral activity. Background technique [0002] my country is a big aquaculture country, but with the continuous development of aquaculture industry in recent years, the incidence of aquatic animal diseases, especially viral diseases, has shown a clear upward trend. This has caused huge economic losses to my country's aquaculture industry and import and export trade, seriously restricting the healthy and sustainable development of my country's aquaculture industry. Among them, Spring viraemia of carp (SVC) is a fulminant hemorrhagic disease caused by Spring viraemia of carp virus (SVCV). The disease is an acute epidemic disease with hemorrhage as the main clinical symptom and highly contagious. It can cause obvious symptoms in many carps such as carp, koi, grass carp, silver carp, bighead carp, cruc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46C12N15/12C12N15/85A61K38/17A61P7/04A61P31/14
CPCC07K14/461C12N15/85A61P7/04A61P31/14A61K38/00Y02A50/30
Inventor 邵玲汤茜肖雨
Owner 上海市水产研究所(上海市水产技术推广站)
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