Beauveria bassiana strain and application thereof

A technology of Beauveria bassiana and strains, applied in the fields of application, fungi, biocides, etc., can solve the problems of difficult physical control, difficult to find, strong flying ability of adults, etc., to achieve strong infection control ability and control the number Effect

Active Publication Date: 2019-08-09
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because jerky worms are drill-piercing pests, larvae live in tunnels under the bark, are not easy to find, and are difficult to control physically; adults have strong flying abilities and avoid spraying pesticides, resulting in unsatisfactory chemical control effects, and environmental pollution
[0004] At present, there is a lack of a Beauveria bassiana bacterial strain with high insecticidal ability against gillworms and its application

Method used

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  • Beauveria bassiana strain and application thereof
  • Beauveria bassiana strain and application thereof
  • Beauveria bassiana strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A strain of Beauveria bassiana of the present invention, the strain is Beauveria bassiana; the preservation name is Beauveria bassiana JXAS-02; it is preserved in the China Typical Culture Collection Center, and the preservation address is Wuhan City, Hubei Province China Type Culture Collection Center of Wuhan University, Bayi Road, Hongshan District; date of deposit: April 25, 2019; deposit number: CCTCC M 2019293.

[0026] The sequence of the internal transcribed spacer of Beauveria bassiana JXAS-02, that is, the nucleotide sequence of the ITS sequence is shown in SEQ ID NO:1.

[0027] The Beauveria bassiana bacterial agent prepared by the Beauveria bassiana of the present invention.

[0028] The active ingredient of the Beauveria bassiana agent is at least one of the following (a), (b) and (c):

[0029] (a) the fermentation culture of said Beauveria bassiana;

[0030] (b) Ultrasonic lysed supernatant of the resulting Beauveria bassiana cells;

[0031] (c) Sonic l...

Embodiment 2

[0039] The difference between Example 2 and Example 1 is that a method for isolating the bait of Beauveria bassiana of the present invention comprises the following steps: in step (2), burying the larvae of the larvae in the soil, Place it in a constant temperature and humidity incubator with a temperature of 30°C and a relative humidity of 80%. After the jerky worms are parasitized by fungi and become dead worms, take out the dead worms and place each worm in a well-soaked filter paper. In the petri dish, wait for the fungal fruiting bodies to fully grow.

Embodiment 3

[0041] The difference between Example 3 and Example 1 is that a method for isolating the bait of Beauveria bassiana of the present invention comprises the following steps: in step (2), burying the larvae of the larvae in the soil, Place it in a constant temperature and humidity incubator with a temperature of 25°C and a relative humidity of 80%. After the worms are parasitized by fungi and become dead worms, take out the dead worms and place each worm in a well-soaked filter paper. In the petri dish, wait for the fungal fruiting bodies to fully grow.

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Abstract

The invention discloses a beauveria bassiana strain and application thereof. The beauveria bassiana strain is beauveria bassiana; the preservation name is Beauveria bassiana JXAS-02; the beauveria bassiana strain is preserved in Chinese Type Culture Collection Center at Wuhan University, Bayi Road, Hongshan District, Wuhan City, Hubei Province; the preservation date is April 25, 2019; the preservation number is CCTCC M 2019293. The beauveria bassiana strain JXAS-02 having the high-efficiency insecticidal capability to flatheaded borers is separated and identified from soil by taking flatheadedborer larvae as baits through a biological trapping method from the perspective of biological control, the bacterial agent has the strong infection prevention and control capability on larvae and adults of flatheaded borers, and the strain can be used for effectively controlling the population quantity of flatheaded borers by spraying or pouring a conidium suspension liquid of the strain into feeding tunnels of the flatheaded borers.

Description

technical field [0001] The invention relates to the technical field of insect and microorganism control, in particular to a Beauveria bassiana JXAS-02 susceptible to G. spp. and the application of the bacterium in the prevention and control of G. spp. pests. Background technique [0002] Coleoptera belongs to the order Coleoptera, and the adult insects live on woody plants and lay eggs in the crevices of bark. Most of the larvae feed on the inner bark of the branches or roots. When the damage is serious, the bark bursts, so it is called blaster. Most of the column roads are wide and flat, with a lot of sawdust-like feces. Generally, one generation occurs in one year, and the mature larvae survive the winter in the column, and the next year they pupate and emerge to go out. Adults often crawl on tree trunks and feed on leaves to supplement nutrition on tree crowns. After mating 1-2 days, they lay eggs on the tree trunk. After hatching, the larvae sneak under the bark and f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCC12N1/14C12R2001/645C12N1/145A01N63/30
Inventor 占智高关丽梅郭燕王金昌靳亮李鹏张婷吕珺
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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