A method for detecting red blood cell DNA damage signal and its application in lymphoma prognosis

A technology of lymphoma and signaling, applied in the field of medicine and biology, to achieve the effect of improving detection efficiency, reducing pain, and widely used

Active Publication Date: 2020-06-09
杭州太铭生物科技有限公司
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Problems solved by technology

Due to the diversity of DLBCL pathogenic factors, age of onset, and disease grading, the chemotherapy regimen needs to be followed up in real time. However, the evaluation of the chemotherapy effect at this stage still needs to be based on the doctor's subjective experience and the pathological results obtained after puncture. This method is repeated for patients. Invasive puncture procedures are also a pain for patients

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  • A method for detecting red blood cell DNA damage signal and its application in lymphoma prognosis
  • A method for detecting red blood cell DNA damage signal and its application in lymphoma prognosis
  • A method for detecting red blood cell DNA damage signal and its application in lymphoma prognosis

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[0028] In order to make the purpose, technical solutions and advantages of the present invention clearer, the following in conjunction with specific examples, and with reference to the appended Figure 1-10 , to further describe the present invention in detail.

[0029] Before introducing the specific implementation content of the present invention, the nouns involved in this implementation content are briefly explained and introduced:

[0030] Erythrocyte DNA damage signal: Mature erythrocytes have no nucleus. If in the early stage of erythrocyte maturation, chromatids or chromosomes are acentric fragments, or the entire chromosome is lost due to spindle damage, in the late stage of cell division, some DNA nucleic acid fragments remain in the cytoplasm, that is to say, these residual DNA signals will form one or several regular subnuclei in the cytoplasm of mature erythrocytes, and these subnuclei are residual DNA damage signals.

[0031] CD235a: Glycophorin A, is a single t...

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Abstract

The invention belongs to the field of medical biology, and particularly relates to a method for detecting red blood cell DNA damage signals. The method comprises the following steps that S1, blood samples are prepared; S2, control groups and experimental groups are cleaned and treated; S3, the second experimental group is subjected to dyeing treatment; S4, the second control group is subjected toresuspending treatment; S5, the third experimental group and the third control group are detected by a flow method; and S6, results are analyzed. The invention further provides application of the method to lymphoma prognosis. The changes of the proportion of the own DNA damage signals of a patient are compared, a DNA damage signal database of healthy population and lymphoma disease population is established, and the DNA damage signals of lymphoma disease patients are rapidly and effectively compared to the database to assess the prognosis of the lymphoma disease patients. The method of rapidlydetermining the DNA damage signals in red blood cells damaged in early and medium stages of the lymphoma patients by traces of blood samples and reagents has the advantages of high specificity and high sensitivity.

Description

technical field [0001] The invention belongs to the field of medicine and biology, and specifically relates to a method for detecting red blood cell DNA damage signals and its application in lymphoma prognosis. Background technique [0002] Lymphoma is a type of hematopoietic malignant tumor caused by lymphocyte (a type of white blood cell) lesions. Clinical symptoms generally include lymph node enlargement but no pain, fever, night sweats, weight loss, itching and fatigue and other systemic symptoms. Lymphoma has many subtypes according to different classification methods. The main classification method is to divide it into non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL) according to the tumor cells. The pathological features of HL include lymphocytes, eosinophils, plasma cells and specific Reed-Steinberg cells in the tumor tissue. The incidence of NHL is much higher than that of HL, accounting for about 90%, which is the sum of a group of independent diseases wit...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/80G01N15/14
CPCG01N15/14G01N33/58G01N33/6842G01N33/80G01N2015/1486G01N2015/149
Inventor 葛建萍李丹丹
Owner 杭州太铭生物科技有限公司
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