31results about How to "Realize trace detection" patented technology

The invention discloses a microfluidic chip for sol-based SERS detection and a method for using the same. The microfluidic chip includes a solution inlet and a solution outlet, and a detection area is arranged between the solution inlet and the solution outlet. , it is characterized in that: the detection area includes a sandwich structure, the upper and lower layers are quartz layers, the middle layer is a film layer, the film layer is provided with a hollow channel as the detection area, the hollow channel communicates with the solution inlet and the solution outlet, and the hollow channel The height is 10‑100 μm, the width is 40‑200 μm, the thickness of the upper layer is 300‑1000 μm, and the thickness of the lower layer is 100‑500 μm. Mix Au@SiO2 nanoparticles with a coating degree of 40-70% and the analyte, adjust the aggregate particle size of the system to 100 μm-300 μm with equivalent sulfuric acid and NaOH, and pass it into the chip for SERS detection. The invention can improve the repeatability of SERS detection.

The invention relates to a method for detecting antigen content in a sample based on immunohistochemical spatial electrochemical detection, comprising the following steps: modifying electrochemical signals (ferrocene / quantum dots, etc.) For the bound antibody, an electrochemical instrument is used to detect the electrochemical signal in the solution, and the strength of the signal reflects the content of the antigen in the cancer tissue / cell. Compared with the prior art, the present invention can realize the simultaneous detection of different antigens by using different electrochemical signals to modify antibodies; through the determination of the standard curve, the electrochemical signal value directly corresponds to the number of antigens, realizing full quantitative detection; Compared with immunohistochemistry, the detection steps are simple to operate and require less technical skills for the operator; the modified electrode can greatly improve the sensitivity and realize the detection of micro-antigens.

The invention discloses a biological sensor, which comprises a sensor wafer, a measuring pad point, a differential inductance coil, an air bridge structure, a point array capacitor, a capacitor upper plate, and a capacitor lower plate. The preparation method is as follows: deposit the gallium nitride dielectric layer on the surface of the wafer; use Ti / Au as the first layer of seed metal; use photoresist to define the underlying metal, SiNx dielectric layer, air bridge column, and air bridge metal. structure, electroplating the lower metal and upper metal on the seed metal; depositing the gallium nitride passivation layer on the surface of the wafer; defining the structure of the contact electrode Pad; grinding, polishing, cutting, and metal jumper bonding, and packaging. The present invention forms a microwave resonator device through intertwining differential inductors and circular point array capacitors in series, which greatly reduces the chip size occupied by the device while improving the quality factor of the device; solves the problem of measuring biomarkers by microwave devices The problem of low sensitivity when the solution concentration is low.

The invention relates to a delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of gas Raman detection. The delta type resonant cavity / integrating sphere combination reinforcing cavity comprises three endoscopes (M1, M2 and M3) with high reflectivity, a gold-plated integrating sphere, inverse reflecting mirror (M4) with high reflectivityand a sealing chamber, wherein the three endoscopes (M1, M2 and M3) with the high reflectivity are inlaid in three holes corresponding to the gold-plated integrating sphere, the inverse reflecting mirror (M4) with the high reflectivity is arranged outside the gold-plated integrating sphere and in rear of the endoscope (M3), and the inverse reflecting mirror (M4) is arranged in the sealing chamber. The delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of the gas Raman detection has the advantages that for the problems that an existing detection device for characteristic gases in a change-over state is low in detection accuracy and cannot measure and distinguish multiple characteristic gases simultaneously, the technical bottleneck that the minimum gas detection concentration of a Raman spectrum cannot meet the requirements is broken through, and the accuracy and sensitivity of the detection of the characteristic gases in the change-over state are greatly improved.

The invention discloses a glucose quantitative testing sensor based on a microwave patch resonator and a preparation method. The sensor solves the problem that a glucose concentration is inaccurate when a microwave device measures the glucose concentration. The method comprises the following steps: firstly manufacturing the patch resonator and a sample patch of a coplanar waveguide feeder on the front side of a semi-conductive substrate, and then manufacturing a fence structure of SU-8 light resistance adhesive on the front side of the substrate containing the sample patch, or manufacturing a groove structure on the back side of the substrate containing the sample patch. The sensor adopts resonant frequency and reflection coefficient as detection parameters, realizes the characteristics that the detection reaction time is not more than one second, the sensitivity is high and the sensor can be reused, and overcomes the disadvantage that generally biosensors based on microwave devices cannot perform quantitative detection.

The present invention relates to a high-sensitivity biosensor and its manufacturing method and application. First, the nano-gold particles or pine-like gold are modified on the surface of the electrode by electroplating, and then the conductive polymer is modified on the surface of gold materials with different morphologies by electrochemical polymerization, by activating the carboxyl groups on the surface of the conductive polymer; finally The activated electrode is immersed in a specific bioidentifier, and the bioidentifier is immobilized through an amide reaction to obtain the high-sensitivity biosensor. Different biomarkers on highly sensitive biosensors can be used for the specific detection of different biomarkers. The invention uses pine tree-like gold or nano-gold and conductive polymers to prepare high-sensitivity biosensors, greatly increases its surface active area, promotes electron transfer, and enhances the electrical conductivity of electrodes; finally, the sensitivity of the biosensor is improved, and the biological marker is realized. Trace detection, the lowest detection limit reaches aM level (10 ‑9 nM).

The invention relates to a quick and repeatable method for detecting trace amounts of hydrazine hydrate, which belongs to the technical field of inorganic detection. The invention provides a method for detecting a trace amount of hydrazine hydrate. The specific method is: adding the substance to be tested into a mixed detection solution of a solvent and elemental sulfur and stirring evenly. If the mixed detection solution can display blue color, it indicates that the substance to be tested contains Hydrazine hydrate; Wherein, the solvent is N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidone or dimethyl sulfoxide, and the mass fraction of elemental sulfur in the mixed detection solution is 0.0001% to 1%. The method for detecting trace amounts of hydrazine hydrate of the present invention can realize the trace detection of hydrazine hydrate repeatedly and rapidly.

The invention relates to a method for quickly and quantitatively detecting elemental sulfur, which belongs to the technical field of inorganic detection. The present invention provides a method for detecting elemental sulfur. The method is as follows: adding the substance to be tested into the mixed detection solution of N,N-dimethylformamide and hydrazine hydrate, and the mixed detection solution shows blue, indicating that the The substance to be tested contains sulfur; wherein, the volume ratio of N,N-dimethylformamide and hydrazine hydrate in the mixed detection solution is 1:1 to 3000:1. The preparation process of the detection solution of the method of the present invention is simple, and can be stored for a long time; and the detection method of the present invention is simple and quick, and the result only needs to observe the color change of the detection solution with the naked eye; trace detection.

The invention provides a surface-enhanced Raman scattering substrate and its preparation method and application, which belong to the technical field of surface-enhanced Raman scattering thin films. The surface-enhanced Raman scattering substrate includes a substrate and a three-dimensional noble metal nanostructure; the substrate It is a NAAO thin film, and the noble metal is Ag; the preparation method is as follows: make Fe on the surface of the NAAO thin film 3+ Complexation with tannic acid (TA) to obtain NAAO@TA / Fe 3+ , and further deposited in situ in a silver-containing solution to form a surface-enhanced Raman scattering substrate. The invention adopts a step-by-step assembly method, and uses a metal polyphenol coordination polymerization network to coat the NAAO film, and the TA / Fe on the surface 3+ The complex deposited large-area uniform silver nanostructures (AgNS) in situ on the NAAO substrate to prepare a surface-enhanced Raman scattering substrate, which has the advantages of uniformity, large area, and high sensitivity, and can realize on-site detection of trace amounts of fluoroacetic acid.

The invention discloses a reduced glutathione fluorescent probe with pyrazoline as a maternal body. The probe is 2-(1, 5-diphenyl-4, 5-dihydro-1H-pyrazole-3-yl)phenyl acrylate. The probe provided by the invention can selectively interact with reduced glutathione, has a fluorescence enhancement effect, can be used for analysis through fluorophotometry and can realize trace detection of reduced glutathione. With high sensitivity, the reduced glutathione fluorescent probe is suitable for wide popularization.