31results about How to "Realize trace detection" patented technology

The invention discloses a magnetic immunochromatographic test strip capable of quantitatively detecting CEA and CA19-9 in blood simultaneously and a preparation method. The test strip is prepared by successively binding a sample pad, a combining pad, a nitrocellulose membrane and water absorbing paper to a bottom plate of the test strip and 2mm of adjacent parts are alternately overlaid one another, wherein the nitrocellulose membrane is pre-coated with a detection line of a CEA antibody and a CA19-9 antibody and a quality control line of a rabbit antimouse IgG antibody. Compared with existingtumor marker detection technologies, the magnetic immunochromatographic technology is creatively introduced into combined quantitative detection of CEA and CA19-9 to achieve microscale quantitative analysis of CEA and CA19-9, so that the detecting sensitivity is improved greatly; and based on double positive CEA and CA19-9, the occurrence rate and transfer rate of tumor can be further judged to improve early stage diagnostic rate of occurrence and transfer of colon cancer to achieve the purpose of immediate detection and quick diagnosis, and the test strip is simple to operate and low in cost, and can be widely popularized.

The invention relates to a preparation method of hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously. The detection problem of the IL-6, IL-4 and TNF-alpha can be effectively solved. The method comprises the following steps: preparing anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibody for hollow gold-silver bimetallic mark and envelope detection lineso as to respectively prepare anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibody hollow gold-silver bimetallic markers, drying and curing the hollow gold-silver bimetallic marker, and orderly enveloping the anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibodies for envelope detection line on a nitrocellulose membrane to form the detection line, enveloping the anti-rat immune globulin on the nitrocellulose membrane to form a quality control line of the envelope membrane, thereby obtaining the test strip. The preparation method disclosed by the invention is simple in process, novel and unique, and capable of quickly, simply, early and effectively diagnosing and identifying stroke.

The invention discloses a preparation method of a carbon dot and an array thereof. A nano-gold macromolecular membrane is illuminated by laser so that nano-gold is subjected to surface plasmon resonance and temperature rising, and a macromolecular structure at the periphery is damaged to form the carbon dot. The method disclosed by the invention is simple to operate and the preparation success rate can reach 100 percent; the prepared carbon dot can send out intensive fluorescent light under the stimulation of light in a range of 220nm to 550nm; the carbon dots can be used for preparing various arrayed carbon dot arrays; the fluorescence intensity of the carbon dot can be controlled through adjusting the laser illumination time and power and controlling the size of the carbon dot; the prepared carbon dot array can form an invisible fluorescence array and encrypted information transmission can be realized. Meanwhile, trace detection of ions or molecules can also be realized by utilizing quenching characteristics of the ions or molecules to be detected on the fluorescent light.

The present invention discloses a nitrite detection method. The method comprises the following steps that: 1) a nitrite-containing material requiring detection and a reagent are placed in a reaction tank, the nitrite reacts with the reagent to generate nitrogen gas; 2) the pressure of the nitrogen gas is detected; 3) the concentration of the nitrite in the material requiring detection is calculated according to the pressure of the nitrogen gas. According to the present invention, the reagent is adopted to carry out the reaction with the nitrite to generate the N2; after completing the reaction, the pressure value is detected, and the nitrite content is calculated; the reaction of the reagent and the nitrite is performed in a closed reaction tank, and the N2 pressure is detected; according to the gas state equation, the pressure is proportional to the amount of the N2, and the amount of the N2 is proportional to the concentration of the nitrite, such that the nitrite concentration in the water body can be obtained by detecting the N2 pressure. With the present invention, the wide range detection of the nitrite can be achieved, and both the micro detection and the constant detection can be achieved. The present invention further discloses an automatic nitrite analyzer adopting the detection method.

The invention discloses a microfluidic chip for sol-based SERS detection and a method for using the same. The microfluidic chip includes a solution inlet and a solution outlet, and a detection area is arranged between the solution inlet and the solution outlet. , it is characterized in that: the detection area includes a sandwich structure, the upper and lower layers are quartz layers, the middle layer is a film layer, the film layer is provided with a hollow channel as the detection area, the hollow channel communicates with the solution inlet and the solution outlet, and the hollow channel The height is 10‑100 μm, the width is 40‑200 μm, the thickness of the upper layer is 300‑1000 μm, and the thickness of the lower layer is 100‑500 μm. Mix Au@SiO2 nanoparticles with a coating degree of 40-70% and the analyte, adjust the aggregate particle size of the system to 100 μm-300 μm with equivalent sulfuric acid and NaOH, and pass it into the chip for SERS detection. The invention can improve the repeatability of SERS detection.

The invention relates to a method for detecting antigen content in a sample based on immunohistochemical spatial electrochemical detection, comprising the following steps: modifying electrochemical signals (ferrocene / quantum dots, etc.) For the bound antibody, an electrochemical instrument is used to detect the electrochemical signal in the solution, and the strength of the signal reflects the content of the antigen in the cancer tissue / cell. Compared with the prior art, the present invention can realize the simultaneous detection of different antigens by using different electrochemical signals to modify antibodies; through the determination of the standard curve, the electrochemical signal value directly corresponds to the number of antigens, realizing full quantitative detection; Compared with immunohistochemistry, the detection steps are simple to operate and require less technical skills for the operator; the modified electrode can greatly improve the sensitivity and realize the detection of micro-antigens.

The invention discloses a biological sensor, which comprises a sensor wafer, a measuring pad point, a differential inductance coil, an air bridge structure, a point array capacitor, a capacitor upper plate, and a capacitor lower plate. The preparation method is as follows: deposit the gallium nitride dielectric layer on the surface of the wafer; use Ti / Au as the first layer of seed metal; use photoresist to define the underlying metal, SiNx dielectric layer, air bridge column, and air bridge metal. structure, electroplating the lower metal and upper metal on the seed metal; depositing the gallium nitride passivation layer on the surface of the wafer; defining the structure of the contact electrode Pad; grinding, polishing, cutting, and metal jumper bonding, and packaging. The present invention forms a microwave resonator device through intertwining differential inductors and circular point array capacitors in series, which greatly reduces the chip size occupied by the device while improving the quality factor of the device; solves the problem of measuring biomarkers by microwave devices The problem of low sensitivity when the solution concentration is low.

The invention relates to a delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of gas Raman detection. The delta type resonant cavity / integrating sphere combination reinforcing cavity comprises three endoscopes (M1, M2 and M3) with high reflectivity, a gold-plated integrating sphere, inverse reflecting mirror (M4) with high reflectivityand a sealing chamber, wherein the three endoscopes (M1, M2 and M3) with the high reflectivity are inlaid in three holes corresponding to the gold-plated integrating sphere, the inverse reflecting mirror (M4) with the high reflectivity is arranged outside the gold-plated integrating sphere and in rear of the endoscope (M3), and the inverse reflecting mirror (M4) is arranged in the sealing chamber. The delta type resonant cavity / integrating sphere combination reinforcing cavity capable of improving the sensitivity of the gas Raman detection has the advantages that for the problems that an existing detection device for characteristic gases in a change-over state is low in detection accuracy and cannot measure and distinguish multiple characteristic gases simultaneously, the technical bottleneck that the minimum gas detection concentration of a Raman spectrum cannot meet the requirements is broken through, and the accuracy and sensitivity of the detection of the characteristic gases in the change-over state are greatly improved.

The invention discloses a glucose quantitative testing sensor based on a microwave patch resonator and a preparation method. The sensor solves the problem that a glucose concentration is inaccurate when a microwave device measures the glucose concentration. The method comprises the following steps: firstly manufacturing the patch resonator and a sample patch of a coplanar waveguide feeder on the front side of a semi-conductive substrate, and then manufacturing a fence structure of SU-8 light resistance adhesive on the front side of the substrate containing the sample patch, or manufacturing a groove structure on the back side of the substrate containing the sample patch. The sensor adopts resonant frequency and reflection coefficient as detection parameters, realizes the characteristics that the detection reaction time is not more than one second, the sensitivity is high and the sensor can be reused, and overcomes the disadvantage that generally biosensors based on microwave devices cannot perform quantitative detection.

The invention discloses a triarylamine derivative fluorescent probe as well as a preparation method and application thereof, and relates to a triarylamine derivative fluorescent probe containing a thioacetal structure as well as a synthesis method and application of the probe. The preparation process of the novel triarylamine derivative fluorescent probe comprises the following steps: carrying outcoupling reaction on N-(4-biphenyl)-(9,9-dimethylfluoren-2-yl)amine and p-chlorobenzaldehyde, and carrying out aldehyde thioacetal protection reaction on the obtained product. According to the fluorescent probe, in a capronitrile (ACN) / buffer solution (HEPES) (9 / 1, v / v) solution, Hg<2+> is used for promoting the deprotection effect of thioacetal to cause the fluorescence effect change of a detection system, so that the on-off detection of Hg<2+> is realized. The probe has the advantages of short response time, good ion selectivity, strong interference resistance to other metal ions and the like in the Hg<2+> detection process, meanwhile, the detection limit is as low as 1.57*10<-8> mol / L, and trace detection of Hg<2+> can be realized. The method is applied to Chinese herbal medicine Hg<2+> detection.

The present invention relates to a high-sensitivity biosensor and its manufacturing method and application. First, the nano-gold particles or pine-like gold are modified on the surface of the electrode by electroplating, and then the conductive polymer is modified on the surface of gold materials with different morphologies by electrochemical polymerization, by activating the carboxyl groups on the surface of the conductive polymer; finally The activated electrode is immersed in a specific bioidentifier, and the bioidentifier is immobilized through an amide reaction to obtain the high-sensitivity biosensor. Different biomarkers on highly sensitive biosensors can be used for the specific detection of different biomarkers. The invention uses pine tree-like gold or nano-gold and conductive polymers to prepare high-sensitivity biosensors, greatly increases its surface active area, promotes electron transfer, and enhances the electrical conductivity of electrodes; finally, the sensitivity of the biosensor is improved, and the biological marker is realized. Trace detection, the lowest detection limit reaches aM level (10 ‑9 nM).

The invention discloses a preparation method of a carbon dot and an array thereof. A nano-gold macromolecular membrane is illuminated by laser so that nano-gold is subjected to surface plasmon resonance and temperature rising, and a macromolecular structure at the periphery is damaged to form the carbon dot. The method disclosed by the invention is simple to operate and the preparation success rate can reach 100 percent; the prepared carbon dot can send out intensive fluorescent light under the stimulation of light in a range of 220nm to 550nm; the carbon dots can be used for preparing various arrayed carbon dot arrays; the fluorescence intensity of the carbon dot can be controlled through adjusting the laser illumination time and power and controlling the size of the carbon dot; the prepared carbon dot array can form an invisible fluorescence array and encrypted information transmission can be realized. Meanwhile, trace detection of ions or molecules can also be realized by utilizing quenching characteristics of the ions or molecules to be detected on the fluorescent light.

The invention relates to a quick and repeatable method for detecting trace amounts of hydrazine hydrate, which belongs to the technical field of inorganic detection. The invention provides a method for detecting a trace amount of hydrazine hydrate. The specific method is: adding the substance to be tested into a mixed detection solution of a solvent and elemental sulfur and stirring evenly. If the mixed detection solution can display blue color, it indicates that the substance to be tested contains Hydrazine hydrate; Wherein, the solvent is N,N-dimethylformamide, N,N-dimethylacetamide, N-methylpyrrolidone or dimethyl sulfoxide, and the mass fraction of elemental sulfur in the mixed detection solution is 0.0001% to 1%. The method for detecting trace amounts of hydrazine hydrate of the present invention can realize the trace detection of hydrazine hydrate repeatedly and rapidly.

The invention relates to a method for quickly and quantitatively detecting elemental sulfur, which belongs to the technical field of inorganic detection. The present invention provides a method for detecting elemental sulfur. The method is as follows: adding the substance to be tested into the mixed detection solution of N,N-dimethylformamide and hydrazine hydrate, and the mixed detection solution shows blue, indicating that the The substance to be tested contains sulfur; wherein, the volume ratio of N,N-dimethylformamide and hydrazine hydrate in the mixed detection solution is 1:1 to 3000:1. The preparation process of the detection solution of the method of the present invention is simple, and can be stored for a long time; and the detection method of the present invention is simple and quick, and the result only needs to observe the color change of the detection solution with the naked eye; trace detection.

The invention provides a surface-enhanced Raman scattering substrate and its preparation method and application, which belong to the technical field of surface-enhanced Raman scattering thin films. The surface-enhanced Raman scattering substrate includes a substrate and a three-dimensional noble metal nanostructure; the substrate It is a NAAO thin film, and the noble metal is Ag; the preparation method is as follows: make Fe on the surface of the NAAO thin film 3+ Complexation with tannic acid (TA) to obtain NAAO@TA / Fe 3+ , and further deposited in situ in a silver-containing solution to form a surface-enhanced Raman scattering substrate. The invention adopts a step-by-step assembly method, and uses a metal polyphenol coordination polymerization network to coat the NAAO film, and the TA / Fe on the surface 3+ The complex deposited large-area uniform silver nanostructures (AgNS) in situ on the NAAO substrate to prepare a surface-enhanced Raman scattering substrate, which has the advantages of uniformity, large area, and high sensitivity, and can realize on-site detection of trace amounts of fluoroacetic acid.

The present invention discloses a nitrite detection method. The method comprises the following steps that: 1) a nitrite-containing material requiring detection and a reagent are placed in a reaction tank, the nitrite reacts with the reagent to generate nitrogen gas; 2) the pressure of the nitrogen gas is detected; 3) the concentration of the nitrite in the material requiring detection is calculated according to the pressure of the nitrogen gas. According to the present invention, the reagent is adopted to carry out the reaction with the nitrite to generate the N2; after completing the reaction, the pressure value is detected, and the nitrite content is calculated; the reaction of the reagent and the nitrite is performed in a closed reaction tank, and the N2 pressure is detected; according to the gas state equation, the pressure is proportional to the amount of the N2, and the amount of the N2 is proportional to the concentration of the nitrite, such that the nitrite concentration in the water body can be obtained by detecting the N2 pressure. With the present invention, the wide range detection of the nitrite can be achieved, and both the micro detection and the constant detection can be achieved. The present invention further discloses an automatic nitrite analyzer adopting the detection method.

The invention discloses a reduced glutathione fluorescent probe with pyrazoline as a maternal body. The probe is 2-(1, 5-diphenyl-4, 5-dihydro-1H-pyrazole-3-yl)phenyl acrylate. The probe provided by the invention can selectively interact with reduced glutathione, has a fluorescence enhancement effect, can be used for analysis through fluorophotometry and can realize trace detection of reduced glutathione. With high sensitivity, the reduced glutathione fluorescent probe is suitable for wide popularization.

The invention belongs to the field of medical biology, and particularly relates to a method for detecting red blood cell DNA damage signals. The method comprises the following steps that S1, blood samples are prepared; S2, control groups and experimental groups are cleaned and treated; S3, the second experimental group is subjected to dyeing treatment; S4, the second control group is subjected toresuspending treatment; S5, the third experimental group and the third control group are detected by a flow method; and S6, results are analyzed. The invention further provides application of the method to lymphoma prognosis. The changes of the proportion of the own DNA damage signals of a patient are compared, a DNA damage signal database of healthy population and lymphoma disease population is established, and the DNA damage signals of lymphoma disease patients are rapidly and effectively compared to the database to assess the prognosis of the lymphoma disease patients. The method of rapidlydetermining the DNA damage signals in red blood cells damaged in early and medium stages of the lymphoma patients by traces of blood samples and reagents has the advantages of high specificity and high sensitivity.