Hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously, and preparation method thereof

An IL-6 and test strip technology, applied in the field of medical testing, can solve the problems of inability to carry out clinical diagnosis in time, threat to the health of operators, expensive instruments, etc., achieve good clinical application significance, improve early diagnosis rate and prognosis The effect of monitoring and sensitivity improvement

Pending Publication Date: 2019-11-19
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of traditional radioimmunoassay will pose a certain threat to the health of operators, and radioactive markers will pollute the environment. The improved chemiluminescence detection method can be automated, but the instrument is expensive, which limits its application and promotion. The common ELISA method in the world is complicated to operate and takes a long time, so it cannot be diagnosed clinically in time

Method used

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  • Hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously, and preparation method thereof
  • Hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously, and preparation method thereof

Examples

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Embodiment 1

[0029] Example 1, such as figure 1 and figure 2 As shown, this embodiment provides the preparation and detection of immunochromatographic test strips.

[0030] In a specific embodiment, the paired antibodies of IL-6, IL-4 and TNF-α used are monoclonal antibodies prepared by monoclonal antibody technology. The principle of double-antibody sandwich detection of IL-6, IL-4 and TNF-α antigens is used to detect specimens. When the specimens to be tested contain IL-6, IL-4 and TNF-α antigens, the antigens will firstly interact with the antigens on the binding pad. Hollow gold-silver bimetallic conjugates coupled with IL-6, IL-4 and TNF-α antibodies, as the chromatography progresses, the conjugates move forward to the detection line of IL-6, IL-4 and TNF-α antibodies, IL -6. IL-4 and TNF-α antigens will combine with the coated antibody again to form a double-antibody sandwich complex and accumulate at the detection line. In addition, unbound conjugated IL-6, IL-4 and TNF-α antibodie...

Embodiment 2

[0051] Except that in the preparation step of the immune hollow gold-silver bimetal: the addition amount of EDC and NHS is 5 times the molar amount of the carboxylation degree of the hollow gold-silver bimetal, other steps are the same as in Example 1.

Embodiment 3

[0053] Except that in the preparation step of the conjugate pad, the content of sucrose in the conjugate pad treatment solution used was 10 wt%, other steps were the same as in Example 1.

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Abstract

The invention relates to a preparation method of hollow bimetallic test strip for detecting IL-6, IL-4 and TNF-alpha simultaneously. The detection problem of the IL-6, IL-4 and TNF-alpha can be effectively solved. The method comprises the following steps: preparing anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibody for hollow gold-silver bimetallic mark and envelope detection lineso as to respectively prepare anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibody hollow gold-silver bimetallic markers, drying and curing the hollow gold-silver bimetallic marker, and orderly enveloping the anti-IL-6, IL-4 and TNF-alpha protein conjugate monoclonal antibodies for envelope detection line on a nitrocellulose membrane to form the detection line, enveloping the anti-rat immune globulin on the nitrocellulose membrane to form a quality control line of the envelope membrane, thereby obtaining the test strip. The preparation method disclosed by the invention is simple in process, novel and unique, and capable of quickly, simply, early and effectively diagnosing and identifying stroke.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and relates to a hollow bimetallic test strip for simultaneously detecting IL-6, IL-4 and TNF-α and a preparation method thereof. Background technique [0002] Neuroinflammation is one of the most important pathophysiological mechanisms for brain tissue damage after cerebral ischemia. The immune inflammatory response after cerebral ischemia mainly includes the infiltration of leukocytes such as peripheral blood neutrophils in the brain parenchyma, the activation of endogenous inflammatory cells such as macrophages and microglia, and the activation of TNF. -a, IL-4, IL-6 and a series of secretion and release of inflammatory factors. A large number of studies have shown that drugs that inhibit the immune-inflammatory cascade after cerebral infarction can effectively reduce brain damage and exert neuroprotective effects. Currently, the mechanism of neuroinflammation has become a key targ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531
CPCG01N33/558G01N33/531
Inventor 王莹孙保亮孙景懿李汉霞候亚军袁慧杨明峰
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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