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Fluorescence analysis method for determinating artemisinin content

A technology of fluorescence analysis and artemisinin, which is applied in the field of spectral analysis and detection, can solve the problems of complex operation and high cost, and achieve the effects of precise technical means, low cost, high accuracy and sensitivity

Active Publication Date: 2018-11-20
湘西自治州奥瑞克医药化工有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of complex operation and high cost in the current detection process of artemisinin, and provide a fluorescence analysis method for determining the content of artemisinin based on a new type of nanomaterial graphene quantum dot. The unique properties of graphene quantum dots and the special peroxide bridge structure of artemisinin constitute a simple and sensitive detection system, which realizes the quantitative detection of artemisinin

Method used

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  • Fluorescence analysis method for determinating artemisinin content
  • Fluorescence analysis method for determinating artemisinin content
  • Fluorescence analysis method for determinating artemisinin content

Examples

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Embodiment 1

[0032] Example 1 A fluorescence analysis method for determining the content of artemisinin, specifically as figure 1 As shown, the steps are as follows:

[0033] (1) Add concentration of 0 µM, 4 µM, 10 µM, 40 µM, 60 µM, 100 µM, 140 µM, 180 µM, 200 µM, 600 µM, 1000 µM, 1400 µM, 1800 to 14 centrifuge tubes µM and 2200 µM artemisinin ethanol solution 5 µL; the chemical structure of artemisinin is shown in formula (1);

[0034]

[0035] (2) Add 9 µL of 10 mM p-aminophenylboronic acid solution and 25 µL of 50 mM sodium hydroxide in ethanol solution to the centrifuge tube solution obtained in step (1), mix well, and react at room temperature for 10 min;

[0036] (3) Add 20 µL of 1 mg / mL tyrosinase solution to the centrifuge tube solution obtained in step (2), and react in a water bath at 40°C for 10 min;

[0037] (4) Add graphene quantum dots to the centrifuge tube solution obtained in step (3) to make the concentration 0.5 mg / mL, mix well, and react at room temperature for 25 ...

Embodiment 2

[0039] Example 2 Selective Analysis and Detection Method of Artemisinin

[0040] (1) Add 5 µL of 2000 µM artemisinin, ascorbic acid, uric acid, cysteine, lactic acid, maltose, sucrose, aspartic acid, tryptophan, and glutamic acid to 10 centrifuge tubes;

[0041] (2) Add 9 µL of 10 mM p-aminophenylboronic acid solution and 25 µL of 50 mM sodium hydroxide in ethanol solution to the centrifuge tube solution obtained in step (1), mix well, and react for 10 min;

[0042] (3) Add 1 mg / mL tyrosinase solution to the centrifuge tube solution obtained in step (2), and react in a water bath at 40°C for 10 min;

[0043] (4) Add graphene quantum dots to the centrifuge tube solution obtained in step (3) to make the concentration 0.5 mg / mL, mix well, and react for 25 min;

[0044] (5) Add buffer solution to the centrifuge tubes obtained in step (4), so that the volume of the solution in each test tube is 200 µL, and measure the fluorescence intensity values ​​of the centrifuge tube solution...

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Abstract

The invention belongs to the technical field of spectral analysis and detection, and particularly relates to a fluorescence analysis method using a novel nanomaterial, namely graphene quantum dots, inparticular to a fluorescence analysis method for determinating the artemisinin content. A peroxy bridge structure of artemisinin reacts with p-aminophenylboronic acid, a catalytic oxidation reactionis further performed in the presence of oxygen to form p-iminobenzoquinone, and the fluorescence of the graphene quantum dots is quenched. The fluorescence detection method provided by the invention is low in cost, easy to operate, low in detection limit, good in selectivity and high in sensitivity. By the fluorescence analysis method, application of the graphene quantum dots to the field of medicine analysis and detection is expanded, and a convenient and accurate technical means is provided for evaluation and supervision of the quality of an artemisinin medicine.

Description

technical field [0001] The invention belongs to the technical field of spectral analysis and detection, and in particular relates to a fluorescence analysis method using a novel nanomaterial graphene quantum dot, and in particular to a fluorescence analysis method for determining the content of artemisinin. Background technique [0002] Artemisinin is a sesquiterpene lactone compound containing peroxy groups. It is the safest and most effective antimalarial drug after pyrimethamine, chloroquine and primaquine. It has the characteristics of quick action and low toxicity. At the same time, artemisinin also shows good prospects in the treatment of anti-schistosomiasis, mediation or suppression of humoral immune function, and improvement of lymphocyte transformation rate. With the wide application of artemisinin in the field of biochemical medicine, the market demand for artemisinin is expanding rapidly, which provides an opportunity for unscrupulous traders to sell counterfeit ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 朱树芸闫晓璐王玉花赵先恩
Owner 湘西自治州奥瑞克医药化工有限责任公司
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