A kind of high-sensitivity biosensor and its fabrication method and application

A technology of biosensor and manufacturing method, applied in the field of high-sensitivity biosensor and its manufacture, can solve problems such as narrow detection range, many uncontrollable influencing factors, and complex manufacturing of electrochemical sensors, so as to improve sensitivity, realize trace detection, increase Effect of Surface Active Area

Active Publication Date: 2022-07-05
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-sensitivity biosensor and its production method and application, so as to avoid the problems of complex fabrication of electrochemical sensors and many uncontrollable influencing factors in the prior art, thereby avoiding the narrow detection range of existing detection methods The problem

Method used

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  • A kind of high-sensitivity biosensor and its fabrication method and application
  • A kind of high-sensitivity biosensor and its fabrication method and application
  • A kind of high-sensitivity biosensor and its fabrication method and application

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Experimental program
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Effect test

Embodiment 1

[0068] The preparation process of highly sensitive biosensors is as follows: Figure 4 As shown, the gold electrodes were physically polished with 0.3 μm and 0.05 μm aluminum oxide in turn, and the surface of the electrodes was cleaned with a large amount of deionized water and ethanol to obtain the mirror surface morphology; the electrodes were immersed in piranha etching solution (30% H 2 O 2 :18M H 2 SO 4 Volume ratio = 1:3) for ten minutes, remove surface impurities by chemical polishing; use cyclic scanning method in 0.5M potassium hydroxide (voltage range: -1.5-0.1V, scan rate 100mv / s scan times 20 times) Electrochemical polishing; electrochemical polishing in 0.5M sulfuric acid using chronoamperometry and cyclic voltammetry (chronoamperometry, 2V, 5s; -0.35V, 10s; cyclic voltammetry, voltage range: -0.3-1.6V, The scan rate was 100 mv / s, and the number of scans was 20), the electrodes were cleaned with water and absolute ethanol, and dried with nitrogen.

[0069] Th...

Embodiment 2

[0075] The preparation process of highly sensitive biosensors is as follows: Figure 7 As shown, the gold electrodes were physically polished with 0.3 μm and 0.05 μm aluminum oxide in turn, and the surface of the electrodes was cleaned with a large amount of deionized water and ethanol to obtain the mirror surface morphology; the electrodes were immersed in piranha etching solution (30% H 2 O 2 :18M H 2 SO 4 Volume ratio = 1:3) for ten minutes, remove surface impurities by chemical polishing; use cyclic scanning method in 0.5M potassium hydroxide (voltage range: -1.5-0.1V, scan rate 100mv / s scan times 20 times) Electrochemical polishing; electrochemical polishing in 0.5M sulfuric acid using chronoamperometry and cyclic voltammetry (chronoamperometry, 2V, 5s; -0.35V, 10s; cyclic voltammetry, voltage range: -0.3-1.6V, The scan rate was 100 mv / s, the number of scans was 20), the electrodes were cleaned with water and absolute ethanol, and dried with nitrogen.

[0076] Take 1...

Embodiment 3

[0083] The preparation process of highly sensitive biosensors is as follows: Figure 11 As shown, the gold electrodes were physically polished with 0.3 μm and 0.05 μm aluminum oxide in turn, and the surface of the electrodes was cleaned with a large amount of deionized water and ethanol to obtain the mirror surface morphology; the electrodes were immersed in piranha etching solution (30% H 2 O 2 :18M H 2 SO 4 Volume ratio = 1:3) for ten minutes, remove surface impurities by chemical polishing; use cyclic scanning method in 0.5M potassium hydroxide (voltage range: -1.5-0.1V, scan rate 100mv / s scan times 20 times) Electrochemical polishing; electrochemical polishing in 0.5M sulfuric acid using chronoamperometry and cyclic voltammetry (chronoamperometry, 2V, 5s; -0.35V, 10s; cyclic voltammetry, voltage range: -0.3-1.6V, The scan rate was 100 mv / s, the number of scans was 20), the electrodes were cleaned with water and absolute ethanol, and dried with nitrogen.

[0084] Take ...

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Abstract

The present invention relates to a high-sensitivity biosensor and its manufacturing method and application. First, the nano-gold particles or pine-like gold are modified on the surface of the electrode by electroplating, and then the conductive polymer is modified on the surface of gold materials with different morphologies by electrochemical polymerization, by activating the carboxyl groups on the surface of the conductive polymer; finally The activated electrode is immersed in a specific bioidentifier, and the bioidentifier is immobilized through an amide reaction to obtain the high-sensitivity biosensor. Different biomarkers on highly sensitive biosensors can be used for the specific detection of different biomarkers. The invention uses pine tree-like gold or nano-gold and conductive polymers to prepare high-sensitivity biosensors, greatly increases its surface active area, promotes electron transfer, and enhances the electrical conductivity of electrodes; finally, the sensitivity of the biosensor is improved, and the biological marker is realized. Trace detection, the lowest detection limit reaches aM level (10 ‑9 nM).

Description

technical field [0001] The invention belongs to the technical field of biosensors and applications thereof, and in particular relates to a high-sensitivity biosensor and a manufacturing method and application thereof. Background technique [0002] Chronic disease is a chronic non-communicable disease, which is a general term for a long-term disease, or a class of diseases that develop and form damage over time. Common chronic diseases mainly include cardiovascular and cerebrovascular diseases, neurodegenerative diseases, diabetes and cancer. Chronic diseases can cause damage to important organs such as the heart, brain, liver and kidney of patients, greatly affect the quality of life of patients and their families, and bring huge medical and economic burdens to the society. In 2018, the World Health Organization (WHO) stated that about 41 million people die from chronic diseases every year, which is equivalent to 71% of the total global deaths. Of these, 15 million chronic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/48G01N27/26G01N23/2251
CPCG01N27/3278G01N27/48G01N27/26G01N23/2251
Inventor 秦洁玲王平谭笑
Owner TONGJI UNIV
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