A production method for enriching isothiocyanate content in Brassica sprouts and improving yield

A technology of isothiocyanate and production method, which is applied in the production field of enriching the content of isothiocyanate in Brassica sprouts and increasing the yield, and can solve the problem of affecting the growth of Brassica sprouts and reducing yield And other problems, to achieve good health care function, improve the effect of nutritional value

Active Publication Date: 2021-09-07
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of abiotic stresses such as low temperature, low oxygen, and sulfur-salt stress can increase the content of isothiocyanate in plants, but at the same time it will create a stress environment. Brassica vegetable seeds are very sensitive to external adversity stress environments in the early stages of germination. The existence of biotic stress environment will directly affect the growth of Brassica sprouts, resulting in a greatly reduced yield

Method used

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  • A production method for enriching isothiocyanate content in Brassica sprouts and improving yield

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Select broccoli seeds with full granules and no pests and diseases, soak in 1.5% sodium hypochlorite solution by volume for 15 minutes, then wash with distilled water until neutral pH, and then soak in 3 to 5 times the volume (v / w) of pure water After 4 hours, weigh 300 g of the soaked broccoli seeds, place them in a planting pot, and place them in a constant temperature incubator at 30°C. During the germination period, the following treatments were carried out: 16 hours of light every day, and the remaining 8 hours of darkness. Spray 60 mL of distilled water on the 1st day of germination, and then spray 20 mL of a mixed aqueous solution containing 4 mmol / L zinc sulfate and 5 μmol / L melatonin every 24 hours. On the 4th day of germination, Brassica sprouts with excellent growth and neat buds are obtained, the bud length of a single plant is 20 mm, the fresh weight of a single plant is 30 mg, and the content of isothiocyanate is 250 mg / 100 g dry weight.

Embodiment 2

[0026] Select broccoli seeds with full granules and no pests and diseases, soak in 1.5% sodium hypochlorite solution by volume for 15 minutes, then wash with distilled water until neutral pH, and then soak in 3 to 5 times the volume (v / w) of pure water After 4 hours, weigh 300 g of the soaked broccoli seeds, place them in a planting pot, and place them in a constant temperature incubator at 30°C. During the germination period, the following treatments were carried out: light for 16 hours a day, and keep away from light for the remaining 8 hours. Spray 60 mL of distilled water on the 1st day of germination, and then spray 20 mL of a mixed aqueous solution containing 5 mmol / L zinc sulfate and 10 μmol / L melatonin every 24 hours. On the 4th day of germination, Brassica sprouts with excellent growth and neat buds were obtained. The bud length per plant was 19 mm, the fresh weight per plant was 25 mg, and the isothiocyanate content was 200 mg / 100 g dry weight.

Embodiment 3

[0028] Select broccoli seeds with full granules and no pests and diseases, soak in 1.5% sodium hypochlorite solution by volume for 15 minutes, then wash with distilled water until neutral pH, and then soak in 3 to 5 times the volume (v / w) of pure water After 4 hours, weigh 300 g of the soaked broccoli seeds, place them in a planting pot, and place them in a constant temperature incubator at 30°C. During the germination period, the following treatments were carried out: light for 16 hours a day, and keep away from light for the remaining 8 hours. Spray 60 mL of distilled water on the 1st day of germination, and then spray 20 mL of a mixed aqueous solution containing 6 mmol / L zinc sulfate and 50 μmol / L melatonin every 24 hours. On the 4th day of germination, Brassica sprouts with excellent growth and neat buds were obtained. The bud length per plant was 30 mm, the fresh weight per plant was 35 mg, and the isothiocyanate content was 287 mg / 100 g dry weight.

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Abstract

The invention discloses a production method for enriching the content of isothiocyanate in sprout vegetables of the genus Brassica and increasing the yield. Then germinated with exogenous melatonin combined with zinc sulfate solution to obtain isothiocyanate-rich Brassica sprouts. The invention has the characteristics of simple process, low production cost, high content of isothiocyanate and high output of Brassica sprouts, and is suitable for the production of health food-grade isothiocyanate Brassica sprouts.

Description

technical field [0001] The invention belongs to the technical field of production methods of Brassica sprouts, and in particular relates to a production method for enriching the content of isothiocyanate in Brassica sprouts and increasing the yield. Background technique [0002] Brassica sprouts can be produced not only in traditional family workshops, but also in modern industrial production. Generally, the growth process of Brassica sprouts mainly depends on the nutrients stored in the seeds. In terms of cultivation and management, fertilization is generally not required, and the production and operation are simple. It only needs to ensure its water supply under a suitable temperature environment. Therefore, vermiculite cultivation methods are generally used now. , in certain facility conditions or indoors. [0003] Isothiocyanate is a natural anti-cancer active substance in plants. It has good preventive and inhibitory effects on liver cancer, pancreatic cancer, lung can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G7/06A01N43/38A01N59/16A01P21/00
CPCA01G7/06A01G31/00A01N43/38A01N59/16
Inventor 尹永祺周新勇刘寅方维明程超
Owner YANGZHOU UNIV
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