Application of microbial deodorant

A deodorant bacteria agent and biological deodorization technology, applied in the field of microorganisms, can solve the problems of poor high temperature resistance and low applicability of deodorant bacteria, achieve the effect of good environment, improve immunity and growth speed

Active Publication Date: 2019-08-23
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, many deodorizing bacteria screened in China have shown good deodorizing effect at room temperature. When composting livestock and poultry manure, the high temperature stage is the key stage for high temperature aerobic composting of organic solid waste. During this process, it is oxidized and decomposed, and the release of ammonia and hydrogen sulfide is the highest. However, the current deodorizing bacteria have poor high temperature resistance, and their application in the field of organic composting is not high.

Method used

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  • Application of microbial deodorant
  • Application of microbial deodorant
  • Application of microbial deodorant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Screening and identification of bacterial strains

[0019] NH 3 Selective medium: 50.0g sucrose, 10.0mL ammonia water, KH 2 PO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 0.1g, 1% ZnSO 4 5.0mL, NaCl 2.0g, distilled water 1000mL, sterilized at 121°C for 15min.

[0020] h 2 S selective medium: glucose 5.0g, K 2 HPO 4 0.5g, KNO 3 1.0g, MgCl 2 0.5g, NaCl 0.5g, NH 4 Cl 0.5g, Na 2 CO 3 1.0g, FeCl 2 0.01g, distilled water 1000mL, pH natural, sterilized at 121°C for 15min.

[0021] Screening of deodorant bacteria:

[0022] 1) Weigh 10g of pig manure and 10mL of leachate, add 100mL of NH 3 and H 2 Selective culture medium for S, 30°C, 220r / min shaker culture, replace the medium after 2 days, draw 10mL acclimatization culture solution and add it to 100mL fresh selective medium for second-generation acclimatization, continuous enrichment and acclimatization 4 times. Take 10mL of the enrichment solution in a conical flask containing 90mL of sterile w...

Embodiment 2

[0031] Embodiment 2: single bacteria deodorization

[0032] Single-bacteria culture: inoculate Bacillus flexus Z2 into NA medium, culture at 40-45°C for 14 days, and stir evenly to obtain Bacillus flexus culture fluid; inoculate Bacillus megaterium Z3 into In NA medium, cultivate at 30°C for 14 days, stir evenly to obtain a culture solution of Bacillus megaterium, and the number of bacteria in the two strains reaches 10 8 cfu / mL.

[0033] The test material is fresh pig manure, which is taken from the pig farm of Zhejiang Academy of Agricultural Sciences. 10kg of fresh pig manure is equally divided into 10 parts.

[0034] Control group 1: Put 1kg of fresh pig manure in a flat-bottomed container, flatten it and then evenly spray 10mL of NA culture solution. Place a 50mL small beaker containing 20mL 2% boric acid solution in a flat-bottomed container to absorb ammonia gas, and seal the bottle mouth;

[0035] Control group 2: Put 1 kg of fresh pig manure in a flat-bottomed cont...

Embodiment 3

[0053] Embodiment 3: mixed bacteria deodorization

[0054] The test material is fresh pig manure, and 10kg of fresh pig manure is equally divided into 10 parts,

[0055] Test group 9: Mix the Bacillus flexus culture solution obtained in Example 1 with the Bacillus megaterium culture solution at a ratio of 1:1 to obtain a mixed culture solution 1. Put 1 kg of fresh pig manure in a flat-bottomed container, flatten it and then evenly spray 10 mL of the above-mentioned mixed culture solution 1. Place a 50mL small beaker containing 20mL 2% boric acid solution in a flat-bottomed container to absorb ammonia gas, and seal the bottle mouth;

[0056] Test group 10: Mix the Bacillus flexus culture solution obtained in Example 1 with the Bacillus megaterium culture solution at a ratio of 1:1 to obtain a mixed culture solution 1. Put 1 kg of fresh pig manure in a flat-bottomed container, flatten it and then evenly spray 10 mL of the above-mentioned mixed culture solution 1. Place a 50mL...

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Abstract

The invention discloses bacillus flexus Z2 with the preservation number of CCTCC NO: M2018659 or / and a culture or / and a processed object thereof or / and bacillus megaterium Z3 with the preservation number of CCTCC NO: M2018660 or / and a culture or / and a processed object thereof. The two strains have an oxidation effect on ammonia gas and hydrogen sulfide and are resistant to high temperature. Bacillus flexus dry powder or bacillus megaterium dry powder or a bacillus flexus culture solution or a bacillus megaterium culture solution prepared from the strains is applied to livestock and poultry manure or landfill leachate or the bacillus flexus and the bacillus megaterium are evenly mixed in the proportion of (0-100):(0-100) to be applied to a to-be-treated object needing biological deodorization.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the use of a microbial deodorant agent. Background technique [0002] During the composting process of livestock and poultry manure, a large amount of harmful gases will be produced, among which ammonia and hydrogen sulfide are the most important odorous substances. If not handled properly, it will cause serious pollution to the surrounding air, endanger the health of surrounding residents, and cause pollution to the livestock and poultry breeding environment. Deterioration directly affects the healthy development of the aquaculture industry. The release of ammonia and hydrogen sulfide will also reduce the content of nitrogen and sulfur in compost products, affecting the quality of compost products. Therefore, effective measures should be taken to reduce the release of ammonia and hydrogen sulfide during the composting process and convert them into usable substances. [...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/02C02F103/20C12R1/11C12R1/07
CPCC02F11/02C02F2103/20C02F2303/02C12N1/20C12N1/205C12R2001/11
Inventor 沈琦吴逸飞汤江武姚晓红孙宏李园成王新
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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