Magnetic nanoparticle, preparing method thereof and application thereof to transfection agent

Abstract

The invention discloses a magnetic nanoparticle, a preparing method thereof and application thereof to a transfection agent. The method includes the steps of synthesizing a magnetic nanoparticle through a coprecipitation method, adding the magnetic nanoparticle to a hydroxyl ion solution to obtain a ferroferric oxide nanoparticle modified by hydroxyl ions, and adding the magnetic nanoparticle modified by hydroxyl ions to a metal cation solution to obtain the magnetic nanoparticle with positive charges on the surface for magnetic transfection. The magnetic nanoparticle synthesized through the method is small in particle size and suitable for endocytosis; the positive charges are distributed on the surface of the magnetic nanoparticle and can be combined with nucleic acid through the electrostatic interaction, and the nucleic acid concentrating and folding effect is realized. When the magnetic nanoparticle is used for magnetic transfection, the safety risk of transfection to cells is effectively reduced, and the transfection efficiency is further enhanced.

Description

technical field [0001] The invention relates to the field of magnetic material and nanotechnology research, more specifically, to a magnetic nanoparticle, its preparation method and its application in transfection reagents. Background technique [0002] Gene transfection is the process of transferring or transporting nucleic acids with biological functions into cells and maintaining their biological functions. Gene transfection technology has been widely used in genome function research and gene therapy research. The former mainly includes research on gene expression regulation, signal transduction and drug screening, while the latter mainly focuses on gene therapy for tumors, AIDS and genetic diseases. Due to the large size and negative charge of nucleic acids, it is difficult to cross the negatively charged cell membrane through osmosis alone. Even if they enter the cell membrane through endocytosis, nucleic acids are likely to be degraded by lysosomes. In addition, ther...

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Problems solved by technology

[0005] In view of the defects of the prior art, the purpose of the present invention is to solve the complex operation of the magnetic nanoparticle and nucleic acid binding method used in the existing magnetic transfection technology, and the modification of the magnetic nanoparticle with a cationic polymer leads to cytotoxicity and non-specific interaction of blood components. Technical issues limiting clinical application

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