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A prognostic marker for cardiac cancer and its application

A cardia adenocarcinoma and prognosis technology, which is applied in the field of cardiac cancer prognosis prediction markers, can solve the problems of few molecular markers, unaccepted clinical application, poor prognosis of cardia cancer, etc., and achieves simple operation and convenient prognosis prediction. , easy to use effect

Active Publication Date: 2020-02-21
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prognosis of cardia cancer is very poor, and the 5-year survival rate of patients in the middle and advanced stages is less than 25%.
At present, in clinical practice, the prognostic grading of cardiac cancer is mainly based on pathological grade as the most important prognostic indicator, but few prognostic molecular markers have been found, and none of the discovered indicators have been clinically accepted and applied. Patient prognosis prediction is still in the preliminary exploration stage

Method used

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  • A prognostic marker for cardiac cancer and its application
  • A prognostic marker for cardiac cancer and its application
  • A prognostic marker for cardiac cancer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: A method for detecting the expression level of ERICH3 protein, a prognostic marker for cardiac cancer

[0037] The expression level of ERICH3 protein was detected by immunohistochemical method, and the specific steps were as follows:

[0038]1. Fabrication of tissue chip: Cardiac cancer and adjacent normal tissues were fixed with 10% neutral formalin fixative for 48 hours, washed with tap water, and dehydrated with gradient alcohol (70% ethanol for 2 hours, 80% ethanol for 2 hours, 90% ethanol for 2 hours, Ethanol for 3 hours, 95% ethanol for 3 hours, absolute ethanol I for 2 hours, absolute ethanol II for 1 hour), xylene transparent (xylene I for 2 hours, xylene I for 2 hours), 54~58°C paraffin wax dipping (wax dipping I ( 54~56℃) for 30 minutes, soak in wax II (56~58℃) for 1 hour, soak in wax III (56~58℃) for 1 hour), embed into wax blocks, cut into 5um thick slices with a microtome and paste on Place the slides in a dry oven at 65°C overnight, and then pe...

Embodiment 2

[0045] Example 2: Application of ERICH3 protein in prognosis prediction of cardiac cancer

[0046] 1. Retrospective study: Immunohistochemical detection of ERICH3 protein expression in cardia cancer tumor tissue and adjacent normal tissue

[0047] Tissue microarrays were prepared from 150 cardiac cancer tumor tissue samples and their paired adjacent normal tissues, in which 2 spots were taken for the tumor tissue of each patient, and 2 spots were taken for the adjacent normal tissues. The expression level of ERICH3 protein was detected by the immunohistochemical method described in Example 1, and scored and analyzed.

[0048] (1) sample

[0049] All patients were diagnosed after pathological diagnosis and clinical diagnosis; the patient's tumor tissue samples and paracancerous tissue samples were surgical resection specimens; after the samples were collected, they were routinely made into paraffin specimens, sliced, and after HE staining, the area rich in cancer tissue was se...

Embodiment 3

[0095] Example 3: Preparation of a kit for prognostic prediction of cardiac cancer

[0096] A kit for predicting the prognosis of cardia cancer. The kit contains an antibody capable of specifically binding to the ERICH3 protein. The antibody is purchased from Thermo Fisher Scientific, and the article number is PA5-55933. The kit uses immunohistochemical method to quantitatively detect the ERICH3 protein in the surgically resected tissue samples or endoscopic biopsy tissue samples of cardiac cancer, and determines the expression level of ERICH3 protein in the surgically resected tissue samples or endoscopic biopsy tissue samples of cardiac cancer. And according to the expression level of ERICH3 protein to distinguish the length of survival of cardiac cancer patients. Further, the kit also contains a detection buffer and a chromogenic reagent.

[0097] The kit judges the expression level of the ERICH3 protein according to the immunohistochemical staining score of the detected t...

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Abstract

The invention belongs to the technical field of the tumor medicine, and specifically discloses a gastric cancer prognosis predictive maker-ERICH3 protein and application of a detection reagent of theERICH3 protein in preparation of a kit or reagent for gastric cancer prognosis prediction. The detection reagent of the ERICH3 protein is used for detecting whether the ERICH3 protein is expressed molecule. By detecting the expression of the ERICH3 protein in a tissue sample, the lifetime of the gastric cancer patient can be effectively distinguished, thereby providing a new path for the judgementof the gastric cancer prognosis prediction and providing reference evidence for gastric cancer condition analysis by the clinician.

Description

technical field [0001] The invention relates to the technical field of prognosis prediction of cardiac cancer, in particular to a cardiac cancer prognosis prediction marker and application thereof. Background technique [0002] Cardiac carcinoma is one of the common malignant tumors in gastroenterology. It has been reported in the literature that since the 1980s, the incidence of primary adenocarcinoma of the esophagus and cardia cancer has shown an obvious upward trend, but the incidence of tumors in the distal part of the stomach has shown a downward trend. In the past 30 years, primary adenocarcinoma and The incidence rate of cardia cancer is the fastest growing among all malignant tumors, rising nearly 6 times. Epidemiological data studies in my country show that cardia cancer and esophageal cancer have similar geographical distribution characteristics and similar main clinical symptoms (progressive dysphagia). The clinical boundary between cardia cancer and esophageal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446
Inventor 王立东靳艳赵学科宋昕周福有李秀敏鲍启德范宗民王苒李贝韩雪娜郑颖娟纪爱芳
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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