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Group of cardia adenocarcinoma prognosis prediction markers and application thereof

A technology for cardiac adenocarcinoma and prognosis, applied in the field of prognostic markers for cardiac adenocarcinoma, which can solve the problems of predicting patients with cardiac adenocarcinoma, and achieve the effect of convenient prognosis prediction, good prediction effect, and convenient use

Pending Publication Date: 2020-06-23
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +8
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecular markers that can be used to predict the prognosis of cardia adenocarcinoma are urgently needed in this field. In addition, there is currently no simple and effective method that can more accurately predict the prognosis of patients with cardia adenocarcinoma

Method used

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  • Group of cardia adenocarcinoma prognosis prediction markers and application thereof
  • Group of cardia adenocarcinoma prognosis prediction markers and application thereof
  • Group of cardia adenocarcinoma prognosis prediction markers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The composition of embodiment 1 kit

[0035] The composition of kit of the present invention is as follows:

[0036] (1) Primary antibodies: ZBED5 antibody, CUL5 antibody, AVIL antibody, USP15 antibody, purchased from BD Company in the United States;

[0037] (2) Blocking solution, its preparation method is: buy bovine serum albumin BSA from Biosharp company, use phosphate buffer saline (PBS solution) with a pH of 7.2 to 7.4 and a concentration of 0.01mol / L as a solvent to prepare a concentration of 5 % (w / v) bovine serum albumin BSA solution;

[0038] (3) 3% (v / v) H 2 o 2 Working solution, its preparation method is: use PBS solution as diluent to dilute 30% H 2 o 2 The solution was diluted to 3% (v / v) H 2 o 2 solution, 30% H 2 o 2 Purchased from Sinopharm Chemical Reagent Co., Ltd.;

[0039] (4) Antibody diluent, composed of phosphate buffered saline (PBS) containing 0.1% (v / v) Tween-20, the preparation method is: dilute Tween-20 1000 times with PBS solution,...

Embodiment 2

[0046] The usage method of embodiment 2 kit

[0047] Taking formaldehyde-fixed paraffin-embedded tissue sections as an example, the method for using the detection kit of the present invention is as follows:

[0048] 1. Processing of tissue slices

[0049] (1) Dewaxing to water: The tissue sections were immersed in xylene for 5 minutes, absolute ethanol for 5 minutes, 95% ethanol for 5 minutes, 75% ethanol for 5 minutes, and PBS solution for 5 minutes.

[0050] (2) Block endogenous enzyme activity: drop 3% H on the slice 2 o 2 Working solution, can cover the whole tissue section. Place at room temperature for 10 min to inactivate endogenous horseradish peroxidase. Afterwards, the slices were submerged in PBS solution and washed 3 times for 5 min each time.

[0051] (3) Antigen retrieval: Immerse the slices in 0.01mol / L sodium citrate buffer solution, put them into a pressure cooker filled with an appropriate amount of distilled water, and start timing for 5-10 minutes when...

Embodiment 3

[0061] Example 3 Analysis of the prognostic value of the kit of the present invention for cardiac adenocarcinoma

[0062] (1) Test materials

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Abstract

The invention belongs to the technical field of biology, and relates to a cardia adenocarcinoma prognosis prediction technology, in particular to a group of cardia adenocarcinoma prognosis predictionmarkers and application thereof, namely the application of the detection reagent of specifically detecting ZBED5, CUL5, AVIL and USP15 or the group of gene expression products in the cardia adenocarcinoma prognosis prediction kit. A detection object of the kit is a cardia adenocarcinoma surgical excision tissue sample or an endoscopic biopsy tissue sample. The kit provided by the invention is usedfor prognosis prediction of cardia adenocarcinoma, so that prognosis prediction of cardia adenocarcinoma is more convenient and feasible; in addition, the prediction sensitivity of the combination ofthe four markers to the three-year life cycle of the cardia adenocarcinoma patient is 89.1%, and the specificity is 70.2%.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a prognosis prediction technology for cardiac adenocarcinoma, in particular to a group of cardiac adenocarcinoma prognosis prediction markers and applications thereof. Background technique [0002] Cardia adenocarcinoma (GCA) is one of the most common malignant tumors of the upper gastrointestinal tract in northern my country, and its morbidity and mortality are increasing year by year in my country. Prominent epidemiological features of cardiac adenocarcinoma. The onset of cardia adenocarcinoma is insidious, many patients have no obvious symptoms in the early stage, and many patients are already in the middle and late stages when they are diagnosed. At present, the prognosis of cardia adenocarcinoma is very poor. The 5-year survival rate of patients in the middle and advanced stages is only about 20%, which seriously threatens the life and health of the people. In addition, patients g...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/574
CPCG01N33/68G01N33/577G01N33/57407G01N2800/52
Inventor 王立东孟超龙赵学科宋昕李志强张立国张永梅马磊徐全晓李恩民刘太江张高富周福有李秀敏任书伟许丽艳
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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