Topical dressing composition for the treatment of damaged skin tissue
A composition and topical technology, applied in dressings, skin diseases, drug combinations, etc., can solve problems such as instability and vitality damage of mesenchymal stem cells
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Embodiment 1
[0092] Example 1: Topical Bandage / Patch Composition of Mesenchymal Stem Cells
[0093] Mesenchymal stem cells from individual adipose tissue were washed with 0.01M PBS, and transferred to the bottom of a disposable culture flask pre-wetted with 10% FBS complete medium (DMEM medium supplemented with 10% FBS), and incubated at 37 °C, 5% CO 2 , Cultivate in a humid environment for 1-2 hours. The medium was changed every three days. When the cells reached >80% confluency, trypsinize the cells with 0.25% (w / v) trypsin and 0.02% (w / v) EDTA and dilute 2 × 10 4 cells / cm 2 density for subculture.
[0094] Prepare mesenchymal stem cells (approximately 100×10 8 cells) and 5 mL of 2% sodium alginate suspension.
[0095] Method of preparing a topical dressing composition in the form of a bandage / patch:
[0096] a) placing the sterile gauze sheet on the stainless steel mold;
[0097] b) applying mesenchymal stem cells and alginate matrix to said sterile gauze sheet;
[0098] c) put...
Embodiment 1A
[0103] Example 1A: Use of the topical bandage / patch composition of Example 1 in wound healing.
[0104] Topical bandages / patches have been used to treat damaged skin tissue, ie, wounds, in individuals. Application of the topical dressing composition of Example 1 is suitable for autograft or allograft. According to Example 1, a topical bandage / patch was prepared under sterile conditions. Before applying the bandage / patch, gently rinse the wound with sterile saline to remove debris. A topical patch containing mesenchymal stem cells is applied to the injured skin surface. Leave the topical bandage / patch on the wound for 3 to 7 days. Topical bandage / patch application has been repeated at least 3 times until wound closure is observed. From Table 1, Table 2 and figure 1 As evident from the results, it was observed at the end of the 3 month treatment that the wound area had been significantly repaired and the wound was completely closed.
[0105] Table 1
[0106]
[0107] T...
Embodiment 1B
[0109] Example 1B: Viability stability data of mesenchymal stem cells.
[0110] The differentiation capacity of isolated and cultured mesenchymal stem cells was assessed using adipogenic, chondrogenic and osteogenic differentiation media. Cell surface markers were assessed by flow cytometry. Cell viability was determined by dye exclusion 7AAD analysis in flow cytometry.
[0111] For the study, the six topical dressing compositions mentioned in Table 3 (C1 , C2, C3, C4, C5 and C6) were prepared following the procedure given in Example 1 .
[0112] table 3
[0113]
[0114] It is evident from Table 2 that at least about 80% of the mesenchymal stem cells remained viable when the composition was stored at about 4°C and 25°C for 12 months.
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