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Analysis and detection method of triazine compound

A triazine compound and detection method technology, applied in the field of high-performance liquid chromatography analysis, can solve the problems of affecting the detection results, inconvenient operation, long detection time, etc., and achieve the effect of simple mobile phase configuration and short time

Active Publication Date: 2021-07-02
湖南华腾制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the traditional technology, triazine compounds are usually chromatographic columns (C18, 4.6x250mm, 5μm) filled with octadecylsilane-bonded silica gel, with methanol, acetonitrile, and water mixed solution system as mobile phase, and the detection wavelength is 254nm, it takes a long time to analyze and detect by high performance liquid chromatography, the mobile phase configuration is complicated, and the operation is inconvenient
And the mobile phase is isocratic, it is easy to cause the unknown impurity and the main peak to be separated, resulting in the undetected impurity
And the solubility of the triazine compound is poor, difficult to dissolve, affecting the detection results

Method used

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  • Analysis and detection method of triazine compound
  • Analysis and detection method of triazine compound
  • Analysis and detection method of triazine compound

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Instruments and equipment: HPLC detection detector waters 2695

[0033] Chromatographic conditions The chromatographic conditions are Eclipse XDB-C18 4.6X250mm 5μm;

[0034] The detector wavelength is 254 nm. Flow rate: 0.8ml / min Column temperature: 35°C

[0035] Need testing solution: Need testing solution: Accurately weigh 10mg sample and put it in a 10mL volumetric flask, dissolve it with blank solution and set the volume to the mark, and ultrasonically dissolve completely to obtain 1mg / mL need testing solution.

[0036] Blank solution: acetonitrile: 0.03M ammonium acetate (ph8.5) = 9:1 (V / V)

[0037] Injection volume: 5μl

[0038] Sample analysis: each of the blank solution and the test solution was injected into 1 needle.

[0039] Gradient elution program:

[0040] Mobile phase: A: 0.01% formic acid in water, B: acetonitrile

[0041]

[0042] Use the area normalization method to calculate the purity and maximum simple impurity, the result is as follows fi...

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Abstract

The invention discloses an analysis and detection method of a triazine compound, which uses HPLC liquid chromatography for analysis and detection. The analysis and detection method of the invention can effectively separate the triazine compound and its impurities, and the HPLC liquid chromatography has the advantages of Good linear relationship, simple operation, good repeatability and durability, stable and reliable results, especially suitable for the analysis and detection of triazine compounds with poor solubility.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography analysis method, in particular to an analysis and detection method for triazine compounds. Background technique [0002] Triazine compound, its structural formula: [0003] [0004] Wherein R is H, C1~C2 alkyl, C3~C4 straight chain alkyl or branched chain alkyl; its salt is selected from hydrochloride, nitrate, phosphate, sulfate, hydrobromide, methanesulfonate , p-toluenesulfonate, malate, tartrate or lactate. The triazine compound has excellent antitumor effect. [0005] So far, there is no analysis and detection method for this triazine compound in domestic and foreign pharmacopoeias and literature. [0006] In the traditional technology, triazine compounds are usually chromatographic columns (C18, 4.6x250mm, 5μm) filled with octadecylsilane-bonded silica gel, with methanol, acetonitrile, and water mixed solution system as the mobile phase, and the detection wavelength is 254nm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 阳杨尹标林申利群赵洁吴四清
Owner 湖南华腾制药有限公司