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Sugarcane tissue culture seedling outdoor proliferation method

A technology for tissue culture seedlings and sugarcane, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low efficiency of sugarcane tissue culture seedlings, weak external adaptability, low survival rate, etc. The effect of reducing production cost and high survival rate

Active Publication Date: 2019-09-20
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Sugarcane tissue culture seedlings are generally bred by plant tissue culture techniques, such as a method for improving the survival rate of sugarcane tissue culture seedlings (CN101642050A), a method for transplanting sugarcane tissue culture seedlings in the field (CN101707979A), promoting sugarcane tissue culture The high-efficiency seedling raising method (CN104770173A) of early tillering of seedlings is all single plant cultivation, and what obtains is also single plant sugarcane tissue culture seedling, as seen, the efficiency of sugarcane tissue culture plantlet is low, and the sugarcane tissue culture plantlet of cultivating is weak to the external adaptability, The survival rate of planting is low and the cost is high

Method used

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  • Sugarcane tissue culture seedling outdoor proliferation method
  • Sugarcane tissue culture seedling outdoor proliferation method
  • Sugarcane tissue culture seedling outdoor proliferation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] An outdoor propagation method for sugarcane tissue culture seedlings, characterized in that: the method comprises the following steps:

[0049] (1) Tissue culture: in the process of sugarcane tissue culture seedling cultivation, carry out rooting culture when the tissue culture seedling of its indoor culture is proliferated to the third generation or the fourth generation, and the sugarcane tissue culture seedling after rooting is moved to Outdoor artificial plants in artificial plant nurseries;

[0050] (2) remove terminal bud; When the false planting seedling pseudostem height of breeding in false planting nursery is 20cm, the cane leaf more than 2cm of terminal bud is removed with knife, and the terminal bud that is included in the middle of the false diameter at the otch is destroyed, to obtain final product The first generation of sugarcane pseudo-planting mother;

[0051] (3) incision disinfection: spray the incision on the top of the first generation sugarcane p...

Embodiment 2

[0073] An outdoor propagation method for sugarcane tissue culture seedlings, characterized in that: the method comprises the following steps:

[0074] (1) Tissue culture: in the process of sugarcane tissue culture seedling cultivation, carry out rooting culture when the tissue culture seedling of its indoor culture is proliferated to the third generation or the fourth generation, and the sugarcane tissue culture seedling after rooting is moved to Outdoor artificial plants in artificial plant nurseries;

[0075] (2) remove the terminal bud; when the false stem height of the false plant seedlings bred in the false plant nursery is 25cm, the sugarcane leaves more than 2cm above the terminal bud are removed with a knife, and the terminal bud contained in the middle of the false diameter is destroyed by the otch, to obtain final product The first generation of sugarcane pseudo-planting mother;

[0076] (3) incision disinfection: spray the incision on the top of the first generatio...

Embodiment 3

[0098] An outdoor propagation method for sugarcane tissue culture seedlings, characterized in that: the method comprises the following steps:

[0099] (1) Tissue culture: in the process of sugarcane tissue culture seedling cultivation, carry out rooting culture when the tissue culture seedling of its indoor culture is proliferated to the third generation or the fourth generation, and the sugarcane tissue culture seedling after rooting is moved to Outdoor artificial plants in artificial plant nurseries;

[0100] (2) remove the terminal bud; when the false stem height of the false planting nursery breeding is 30cm, remove the sugarcane leaves more than 2cm from the terminal bud with a knife, and destroy the terminal bud contained in the middle of the false diameter at the otch, to obtain final product The first generation of sugarcane pseudo-planting mother;

[0101] (3) incision disinfection: spray the incision on the top of the first generation sugarcane pseudo-planting paren...

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Abstract

The invention relates to the technical field of sugarcane tissue culture seedlings, and particularly discloses a sugarcane tissue culture seedling outdoor proliferation method. The method includes the steps of tissue culturing, top bud removing, incision disinfecting, strain inoculating, hormone processing, water and fertilizer processing, soil covering, repeated processing, rooting, planting and the like. Finally, single sugarcane tissue culture seedlings with roots are prepared. The method has the advantages that the indoor culturing time of the sugarcane tissue culture seedlings is shortened, production cost is reduced, the seedlings can be dug out to be directly planted in a field after proliferation is completed, and the seedlings are high in adaptive capacity to environment and high in survival rate after being transplanted.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of sugarcane seedlings, and in particular relates to an outdoor multiplication method of sugarcane tissue cultured seedlings. Background technique [0002] Sugarcane is a commercial and energy crop in the tropics and subtropics, and is the main raw material for sugar production in my country. The annual sucrose production accounts for about 85% of the sugar production. In sugarcane growing areas, the yield of sugarcane directly affects the local economic development and is the main source of farmers' income. With the depletion of fossil energy, the use of sucrose or bagasse to ferment ethanol as biofuel has broad development space. [0003] The reproduction method of sugarcane is asexual reproduction. In traditional planting, sugarcane seedlings are usually planted directly. Like all other vegetatively propagated crops, long-term cloning can cause sugarcane to accumulate large amounts o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗晟昇唐利球何洪良廖韦卫陈海生韦海球施泽升蒋亚琴彭崇闭德金江清梅秦昌鲜马文清郭强
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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