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Thin-layer chromatography identification of Solamum melongena and quality control method thereof

A quality control method and thin-layer chromatography technique, which are applied in the field of thin-layer chromatography identification and quality control of white eggplant roots, can solve the problems of inconspicuous, uneven, and difficult to break main roots, and achieve strong specificity and separation. Good and easy to operate effect

Inactive Publication Date: 2019-10-01
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] White eggplant root is the dried root and stem base of Solanaceae plant Eggplant; harvested in autumn, remove silt, and dry; mostly fibrous roots, usually curved and interlaced; main root is not very obvious, with several branches, cylindrical; the surface is earthy yellow, Hard texture, not easy to break, yellow-white section; stem base is cylindrical, no more than 5cm long; surface gray-yellow, with fine longitudinal wrinkles and punctate lenticels; leaf scar half-moon shape, slightly raised; light and hard, Difficult to break, yellow-white or light yellow cross-section, fibrous, uneven, light yellow or yellow-white cortex, with light green or gray-white pith in the center; slight gas, light taste; has the effect of clearing away heat and dampness, astringent and hemostasis, mainly used for Symptoms such as chronic dysentery, blood in the stool, edema, leukorrhagia, joint pain, etc., are used by ethnic minorities in Guizhou, widely distributed, and cultivated throughout the country; The invention provides an effective means for judging the authenticity of white eggplant root test products

Method used

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  • Thin-layer chromatography identification of Solamum melongena and quality control method thereof
  • Thin-layer chromatography identification of Solamum melongena and quality control method thereof
  • Thin-layer chromatography identification of Solamum melongena and quality control method thereof

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Experimental program
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Effect test

Embodiment 1

[0042] (1) prepare need testing solution;

[0043] Take 0.5 g of white eggplant root medicinal powder, add methanol 10 ml, ultrasonically extract for 10 min, filter, evaporate the filtrate to dryness, add 1 ml of absolute ethanol to dissolve the residue, and obtain the test solution;

[0044] (2) Preparation of reference substance solution:

[0045] Take 0.5 g of white eggplant root medicinal powder, add methanol 10 ml, ultrasonically extract for 10 min, filter, evaporate the filtrate to dryness, add 1 ml of absolute ethanol to dissolve the residue, and obtain the test solution;

[0046] (3) TLC identification:

[0047] Take 5 μl of each of the reference substance solution and the test solution, and spot the sample on the same thin-layer plate. Butyl acetate: petroleum ether (60-90°C): formic acid is used as the developer, develop, take out, dry in the air, and place under ultraviolet light View under lamp (365nm).

[0048] The mass ratio of the developing agent butyl aceta...

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Abstract

The invention belongs to the technical field of the medicinal material detection, especially a thin-layer chromatography identification of Solamum melongena and a quality control method thereof. The Solamum melongena can be identified through a Solamum melongena thin-layer chromatography identification method, a more reliable quality control method can be determined. Through the Solamum melongenathin-layer chromatography identification method provided by the invention, the obtained atlas has high definition, good separation degree, obvious spot and good reproducibility.

Description

technical field [0001] The invention belongs to the technical field of medicinal material detection, in particular to a TLC identification and quality control method of white eggplant root. Background technique [0002] White eggplant root is the dried root and stem base of Solanaceae plant Eggplant; harvested in autumn, remove silt, and dry; mostly fibrous roots, usually curved and interlaced; main root is not obvious, with several branches, cylindrical; the surface is earthy yellow, Hard texture, not easy to break, yellow-white section; stem base is cylindrical, no more than 5cm long; surface gray-yellow, with fine longitudinal wrinkles and punctate lenticels; leaf scar half-moon, slightly raised; light and hard, Difficult to break, yellow-white or light yellow cross-section, fibrous, uneven, light yellow or yellow-white cortex, light green or gray-white pith in the center; slight gas, light taste; has the effect of clearing away heat and dampness, astringent and hemostasi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/34
CPCG01N1/34G01N30/90
Inventor 陆苑马雪王爱民刘春花张旭孙佳潘洁李勇军王永林兰燕宇
Owner GUIZHOU MEDICAL UNIV
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