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New use of chloroquine in prevention and control of root-knot nematodes

A technology for root-knot nematodes and knot nematodes, applied in nematicides, applications, chemicals for biological control, etc., can solve problems such as environmental pollution and waste of resources

Active Publication Date: 2019-10-08
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although abamectin has a good control effect on root-knot nematodes, at present, the product is mainly processed into emulsifiable concentrate. The spraying of abamectin brings a large amount of organic solvents to the soil, causing serious environmental pollution and waste of resources.

Method used

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  • New use of chloroquine in prevention and control of root-knot nematodes
  • New use of chloroquine in prevention and control of root-knot nematodes
  • New use of chloroquine in prevention and control of root-knot nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: preparation concentration is the chloroquine phosphate buffered saline solution of 0.25 * M9 buffer solution of 250 μ M, 500 μ M, 750 μ M and 1000 μ M respectively, adds the chloroquine phosphate buffered saline solution of 100mL respectively in the sick soil containing root-knot nematode incognita (every cup Contain 1200±80 root-knot nematode J2 larvae in the diseased soil), add 100mL 0.25×M9 buffer solution as a control treatment; seal the mouth of the plastic cup with plastic wrap, wherein there are four holes of the same size at the bottom of each plastic cup , the plastic cups were placed in the shade of the solar greenhouse; 4 days later, the tomato seedlings with 4 true leaves and the same growth were transplanted in the soil of each plastic cup; the soil was kept moist to make the tomato plants of each treatment grow normally After 45 days, pull up the tomato plant, carefully and slightly clean the root system of the tomato plant, carefully count an...

Embodiment 2

[0028] Example 2: Use a pipette gun to add 500 μL of M9 buffer solution to a 35 mm Petri dish, then add 1500 μL of sterile water to make the buffer solution a 0.25× M9 buffer solution, and add 30 μL of Southern Root-knot nematode J2 larvae suspension, and then add chloroquine phosphate mother solution, make the chloroquine phosphate concentration be 250 μ M, 500 μ M, 750 μ M and 1000 μ M respectively, add 30 μ L 0.25×M9 buffer solution as control, will add the root-knot nematode J2 larva The petri dish was placed at a temperature of 28°C, and the survival of M. incognita J2 larvae was counted under a microscope every 24 h (see Table 1); after 96 h, the J2 larvae of M. incognita were collected for each treatment, and each culture The root-knot nematode J2 larvae collected in the dishes were added to two batches of tomato roots (diameter: 8 cm, height 12 cm) with 4 true leaves, and the tomatoes were put into a solar greenhouse for cultivation; the soil was kept moist, so that eac...

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Abstract

The invention discloses a new use of chloroquine in prevention and control of root-knot nematodes, and belongs to the technical field of the prevention and control of root-knot nematodes. The invention relates to a use of chloroquine in prevention and control of root-knot nematodes and a use of chloroquine in inhibition of infection, development and / or oviposition of root-knot nematodes, wherein the chloroquine is chloroquine phosphate, hydroxychloroquine sulfate or quinine sulfate. The invention also relates to a use of chloroquine in preparation of a root-knot nematode pesticide or a root-knot nematode infection, development and / or oviposition inhibitor, wherein the active component of the root-knot nematode pesticide or the root-knot nematode infection, development and / or oviposition inhibitor is chloroquine phosphate, hydroxychloroquine sulfate or quinine sulfate; and the root-knot nematode pesticide or the root-knot nematode infection, development and / or oviposition inhibitor is an aqueous solution of chloroquine phosphate, hydroxychloroquine sulfate or quinine sulfate. According to the present invention, the prevention and control of root-knot nematodes with the aqueous solution of chloroquine are safe, non-toxic and effective.

Description

technical field [0001] The invention relates to a new application of chloroquine for preventing and controlling root-knot nematode, and belongs to the technical field of root-knot nematode prevention and control. Background technique [0002] The harm caused by root-knot nematodes to crops has surpassed that caused by viruses and bacteria, and has become the second largest plant disease after fungi. Moreover, among the ten common plant pathogenic nematodes, the root-knot nematode (Meloidogyne spp.) ranks first in the damage to plants. So far, 106 species of root-knot nematodes have been discovered and registered, among which four types of root-knot nematodes (Meloidogyne incognita, Meloidogyne arenaria, Meloidogyne hapla) , Java root-knot nematode (Meloidogyne javanica)) are more common and widely distributed. With the expansion of the area of ​​protected vegetable protection in northern my country and the warming of the temperature, root-knot nematodes are showing an outb...

Claims

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Application Information

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IPC IPC(8): A01N43/42A01P5/00
CPCA01N43/42
Inventor 陆朝军邹成钢张克勤孟杨
Owner YUNNAN UNIV
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