Application of promoting HDL synthesis in mice based on LCAT protein hydrosulfide modification

A hydrogen sulfhydration and protein technology, applied in the field of biological sciences, can solve ambiguous problems and achieve the effect of increasing HDL levels

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  • Abstract
  • Description
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Problems solved by technology

The basic condition for sulfhydration modification is that the protein contains cysteine, and it is not clear how many proteins in the body can undergo sulfhydration modification

Method used

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  • Application of promoting HDL synthesis in mice based on LCAT protein hydrosulfide modification
  • Application of promoting HDL synthesis in mice based on LCAT protein hydrosulfide modification
  • Application of promoting HDL synthesis in mice based on LCAT protein hydrosulfide modification

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Embodiment Construction

[0028] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0029] 1. Collection of animal samples

[0030] 6-8 week old male apoE - / - and C57BL / 6 mice (about 20 g in weight), provided by the Experimental Animal Center of the Department of Experimental Medicine, Peking University, are clean healthy animals, free to eat and drink. The experimental process of the present invention was carried out in full compliance with the regulations on the management of experimental animals of Peking University Health Science Center and the relevant regulations of the Ethics Committee.

[0031] 2. Sample processing method, making sample model

[0032] (1) Western diet (0.25% cholesterol) for C57BL / 6 mice for 16 consecutive weeks;

[0033] (2) the apoE - / - Paigen diet (1.25% cholesterol) for mice continued for 12 weeks, and mice had free access to food and water.

[0034] The Western diet C57BL / 6 mice were divide...

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Abstract

The invention discloses an application of promoting HDL synthesis in mice based on LCAT protein hydrosulfide modification to the preparation of drugs for the treatment of atherosclerosis. After the LCAT protein is modified by H2S, the activity of the LCAT protein can be enhanced, the maturation of HDL can be accelerated, and the atherosclerosis and other cardiovascular diseases can be relieved. The application demonstrates that an H2S donor given to a high-fat diet fed mice model can increase plasma HDL levels, and it is found that the key link in the process is the occurrence of hydrosulfidemodification of the LCAT protein; the application can provide basic research support for reverse cholesterol transport and correcting dyslipidemia, and a new target for drug development and treatmentof atherosclerosis and other diseases caused by the dyslipidemia can be provided.

Description

technical field [0001] The invention belongs to the technical field of biological sciences, and in particular relates to an application of promoting mouse HDL synthesis based on sulfhydryl modification of LCAT protein. Background technique [0002] High-density lipoprotein (high density lipoprotein, HDL) has specific physiological or pathological functions, such as anti-oxidation, anti-inflammation, protection of vascular endothelium and antithrombotic effects. In clinical trials, various HDL-increasing drugs did not significantly reduce the occurrence of cardiovascular events. In recent years, the academic community generally believes that controlling HDL within a reasonable range, improving HDL function and activity, and mature HDL have greater significance for cardiovascular diseases. [0003] The packaging and maturation process of HDL is more complicated: HDL is mainly synthesized and secreted by the liver and small intestine, and new HDL is packed in discs, mainly cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/45A61P9/10A61P3/06
CPCA61K38/45C12Y203/01043A61P9/10A61P3/06
Inventor 徐文静褚薇薇王艳丽闫爱丽苏兴利
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