Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

UPLC-PDA detection method of ten black powder

A detection method and quantitative detection technology, which is applied in the field of UPLC-PDA detection of Shiheisan, can solve the problems of great harm, large environmental pollution, and inability to quantitatively measure principal components, etc., and achieve high accuracy and reproducibility.

Inactive Publication Date: 2019-11-12
山东省兽药质量检验所
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Adopt this method, the identification result is stable, obvious, good reproducibility, but its unresolved problem: used reagent benzene etc. toxicity is big, and the harm to people is big, and environmental pollution is big, and spot is easily affected by various external factors such as humidity. Factor interference
Unable to quantify principal components

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • UPLC-PDA detection method of ten black powder
  • UPLC-PDA detection method of ten black powder
  • UPLC-PDA detection method of ten black powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of UPLC-PDA detection method of ten black powder, comprises the following steps:

[0036] (1) Weigh the reference substances of geniposide, rutin, and berberine hydrochloride, respectively, and dilute them with methanol to obtain the stock solutions of geniposide, rutin, and berberine hydrochloride;

[0037] Specifically, geniposide reference substance, content 97.5%, provided by China Veterinary Drug Administration, accurately weighed 14.11mg, diluted with methanol and adjusted to 50mL;

[0038] Rutin reference substance, content 91.9%, provided by the China National Inspection Institute, accurately weighed 10.83mg, diluted with methanol and adjusted to 100mL;

[0039] Berberine hydrochloride reference substance, content 86.7%, provided by the China National Inspection Institute, accurately weighed 20.04mg, diluted with methanol and dilute to 100mL;

[0040] Obtain the stock solutions of geniposide, rutin, and berberine hydrochloride respectively;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of veterinary drug detection, and particularly relates to a UPLC-PDA detection method of ten black powder. The UPLC-PDA detection method of ten black powder is characterized in that the ten black powder is prepared from following raw materials based on parts by weight according to the steps of taking and frying 30 parts of rhizoma anemarrhenae, 25 partsof golden cypress, 25 parts of gardenia jasminoides ellis , 25 parts of Sanguisorba officinalis, 20 parts of Sophora japonica Linn, 25 parts of Typha angustifolia, 20 parts of PLatycladus orientalis,25 parts of Trachycarpus fortune and 25 parts of Eucommia ulmoides to black, jointly smashing the mixture with 15 parts of carbonized hair, and performing sieving and uniform mixing; and identifyingby a spectrogram according to an ultra-high performance liquid chromatography-diode array detection method, and performing content quantitative detection by a chromatogram. The UPLC-PDA detection method has the beneficial effects that the constituent in the ten black powder is detected by the method, rapid qualitative and quantitative detection can be performed three main constituents such as geniposide, rutin and berberine hydrochloride according to parameters such as the spectrogram, the chromatogram, retention time and peak area, and the method accuracy and the reproducibility are high.

Description

technical field [0001] The invention belongs to the technical field of veterinary drug detection, and in particular relates to a UPLC-PDA detection method of Shihei powder. Background technique [0002] Regarding the UPLC-PDA detection method of veterinary medicine Shihei powder, Inner Mongolia Veterinary Medicine published "Research on the Quality Identification Method of Veterinary Medicine Shihei Powder" in "China and Mongolia Veterinary Medicine" in the first issue of 1994. In this article, the method of thin layer chromatography is used To detect Shihei Powder, the specific method is as follows: [0003] Use a capillary to absorb the standard solution, reference solution, sample solution and blank solution respectively, and place them on the same chromatographic plate in turn, using benzene-acetone (9:1) as the developing agent, with a spread distance of 13cm, take them out to dry, spray Bake the mixture (0.5:5) of 8% vanillin anhydrous ethanol and sulfuric acid soluti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/74G01N30/06G01N30/04G01N30/34G01N30/86G01N30/14
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/74G01N30/86G01N30/8634G01N2030/042G01N2030/146
Inventor 魏秀丽张志民张传津李有志
Owner 山东省兽药质量检验所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com