Tissue culture method for obtaining regenerated plantlet by using hippeastrum vittatum plateau as explant

A technology for regenerating plants and explants, applied in the field of seedling propagation of herbaceous ornamental plants, can solve the problems of unstable production and low proliferation rate, and achieve the effect of rapid reproduction and broad application prospects

Inactive Publication Date: 2019-11-15
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have obtained the proliferation medium and rooting medium of Hippeastrum, mostly using bulb discs as explants, but the proliferation rate is relatively low; and the adventitious bud differentiation efficiency of the callus proliferation pathway is prone to instability in production

Method used

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  • Tissue culture method for obtaining regenerated plantlet by using hippeastrum vittatum plateau as explant
  • Tissue culture method for obtaining regenerated plantlet by using hippeastrum vittatum plateau as explant
  • Tissue culture method for obtaining regenerated plantlet by using hippeastrum vittatum plateau as explant

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Experimental program
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Effect test

Embodiment 1

[0032] The four parts of Hippeastrum chinensis without bulb, bulb disk with bulb, base of inflorescence axis and top of inflorescence axis were used as explants to compare the pollution rate of different parts of explants and the effect on the induction of adventitious buds of Hippeastrum chinensis.

[0033] Disinfection methods are all adopted "shake with detergent solution for 15 minutes, rinse with running water for 30 minutes, disinfect with 75% ethanol on a sterile operating table for 60 seconds, then disinfect with 0.1% mercury chloride for 22 minutes, and finally wash with sterile water for 6 times. , wash 1min each time". Cultured in induction medium MS+6-BA 2 mg / L +NAA 0.1 mg / L. Each treatment received 100 explants, repeated 3 times, and the contamination rate, induction rate and induction multiple were counted after 45 days.

[0034] The test results are shown in Table 1. From Table 1, it can be seen that the pollination rate of the inflorescence rachis as an explan...

Embodiment 2

[0038] Bulb discs with bulbs were used as explants. After disinfection, they were inoculated on induction media containing different hormone ratios (CPPU, KT, BA and NAA), and the effects of different media on the induction of adventitious buds were compared. The development of explants was observed, and the adventitious bud induction rate and induction multiple were counted after 45 days.

[0039] It can be seen from Table 2 below that on the MS+6-BA 2mg / L+NAA0.1 mg / L medium, the explants developed best, the induction rate was the highest, and the number of induced adventitious buds was the largest.

[0040] Table 2 The effect of induction medium on the induction of adventitious buds of Lycoris safflower

[0041]

Embodiment 3

[0043] Hippeastrum is a perennial flower, and the explants taken are underground parts, so it is difficult to control the pollution rate. In order to reduce the contamination rate of explants, the effects of different combinations of disinfection methods on the contamination rate of explants were compared. Firstly, the pretreatment and disinfection methods were compared. The explants were treated with 84 disinfectant, Amway detergent, and a mixture of penicillin and streptomycin for 20 minutes, and three kinds of disinfectants were used for 10 minutes each for disinfection pretreatment. Then, transfer to the aseptic operating table, soak the explants in 75% alcohol for 60 seconds, and treat them with 0.1% mercury liter for 20 minutes to disinfect the explants. Secondly, to compare the mercury disinfection time, use the Amway detergent solution to shake and soak for 15 minutes, transfer it to the sterile operating table, and soak it in 75% alcohol for 60 seconds. And then trea...

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Abstract

The invention provides a tissue culture method for obtaining a regenerated plantlet by using a hippeastrum vittatum plateau as an explant. The method comprises the following steps that firstly, explant selection is conducted, and then disinfection is conducted; after disinfection, a bulb is cut into 8-12 blocks according to the size and then inoculated into an induction culture medium for adventitious bud induction; the culture temperature is controlled to be 24-26 DEG C, and under the condition that the illumination time is 10-14 hours/day, and the illumination intensity is 2000-2200 lx, normal culture is conducted for 45 days, and leaves and roots are removed from bulblets obtained through proliferation, and the seedlings obtained through adventitious bud development are cut and transferred into a subculture medium for culture for 25-35 days, and finally, the aseptic seedlings are inoculated into a rooting culture medium for rooting culture for 15-25 days. Through the method, the problem about rapid in-vitro propagation of a new hippeastrum vittatum variety can be effectively solved.

Description

technical field [0001] The present invention relates to Hippeastrum ( Hippeastrum rutilum ) tissue culture propagation method, specifically relates to a tissue culture method for obtaining regenerated plants using Hippeastrum bulb discs as explants, and belongs to the technical field of seedling propagation of herbaceous ornamental plants. Background technique [0002] Hippeastrum ( Hippeastrum rutilum ) is a species of Amaryllidaceae Amaryllidaceae. Perennial herb. The bulb is nearly spherical, with 6-8 leaves, drawn out after flowering, bright green, the flower stem is hollow, slightly flat, with white powder; the perianth tube is green, cylindrical, the perianth lobes are oblong, the apex is pointed, and the color varies with the variety , with small scales on the throat. [0003] At present, there are very rich varieties of Hippeastrum, and the market share is dominated by bulbs imported from the Netherlands. Holland Hippeastrum is characterized by large and bright ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 束晓春李乃伟王忠张凤姣王涛吴宝成
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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