A method for tissue culture and rapid propagation using the bulb plate of Amaryllis chinensis as explants

A technique for rapid propagation of Amaryllis jinhua and tissue culture, which is applied in the fields of botany equipment and methods, horticultural methods, plant regeneration, etc., and can solve problems such as poor induction effect, limited inflorescence rachis, and the rapid propagation method of pollution rate cultivation has not yet been established. , to achieve the effect of rapid propagation, high proliferation rate and high survival rate of transplanting

Inactive Publication Date: 2021-06-11
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have obtained the proliferation medium and rooting medium of other species of Amaryllis, most of which use bulbs as explants, and often have the problems of high pollution rate and relatively low proliferation rate
However, the rapid propagation method of Amaryllis plant tissue culture with high proliferation rate and low pollution rate has not yet been established.
In the early stage, the inventor established the in vitro rapid propagation system of Lycoris safflower by using the inflorescence axis of Lycoris safflower as explants. However, there are differences in the application of the same technology to different species of the same genus. Simple application has poor induction effect, and replacement Compared with Lycoris safflower, the number of provenances of Lycoris safflower is more scarce, and the available inflorescence rachis during flowering period is more limited, so the approach of inflorescence rachis is not suitable for changing Lycoris safflower

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Explant Screening

[0022] The bulb disc, inflorescence rachis and petal base of Lycoris chinensis were used as explants to compare the contamination rate of different explants and the effect on the induction of adventitious buds.

[0023] Disinfection methods are all using "washing detergent, shake for 15 minutes, rinse with running water for 30 minutes, disinfect with 75% ethanol on a sterile operating table for 90 seconds, then disinfect with 0.2% mercury chloride for 25 minutes, and finally wash with sterile water for 5 times. Wash for 1 min each time; ". Cultured in induction medium MS+6-BA 2.5mg / L + zeatin 0.3mg / L + NAA 0.2mg / L. 100 explants were connected to each treatment, and the pollution rate, induction rate and induction multiple were counted after 30 days.

[0024] The test results are shown in Table 1. From Table 1, it can be seen that the explant with the lowest contamination rate is the inflorescence rachis, and the explant with the high induction rate...

Embodiment 2

[0029] Induction medium screening

[0030] Bulb disks were used as explants. After disinfection, they were inoculated on induction medium containing different hormone ratios, and the effects of different medium on induction of adventitious buds were compared. The development of explants was observed, and the induction rate and induction multiple of adventitious buds were counted after 30 days.

[0031] It can be seen from Table 2 that on the medium of MS+6-BA 2.5mg / L+zeatin 0.3mg / L+NAA 0.2mg / L, the explants developed best, the induction rate was the highest, and the number of adventitious buds was induced the most .

[0032] Table 2 The effect of induction medium on the induction of adventitious buds by Lycoris chinensis

[0033] Medium formula Induction rate% Adventitious bud multiple MS+zeatin 2mg / L +NAA0.2 mg / L 80.41 6.84 MS+KT 2mg / L +NAA0.2mg / L 77.44 5.12 MS+6-BA 2.5mg / L + Zeatin 0.3mg / L + NAA 0.2mg / L 97.98 15.01 MS+6-BA 2mg / L + Zea...

Embodiment 3

[0035] Disinfection method screening

[0036] Amaryllis amaryllis is a perennial flower, and the explants taken are underground parts, so it is difficult to control the pollution rate. In order to reduce the contamination rate of explants, on the basis of previous studies, the inventors compared the effects of different combinations of disinfection methods on the contamination rate of explants. First, compare the alcohol disinfection time. After the bulbs are pretreated, they are treated with 75% alcohol for 30s, 90s and 150s, respectively, and then treated with 0.2% mercury chloride for 15min. Secondly, to compare the mercury disinfection time, use Amway washing liquid to shake and soak for 15 minutes, transfer to the sterile operating table, and soak in 75% alcohol for 90 seconds. And then treated with 0.2% mercuric chloride for 15min, 25min and 35min respectively to disinfect the explants of Amaryllis chinensis. All sterilized explants were inserted into adventitious bud ...

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Abstract

The invention provides a method for inducing adventitious buds to obtain regenerated plants by using the bulb disc of Amaryllis amaryllis as an explant, comprising the following steps: selection and disinfection of explants, induction of adventitious buds, subculture and multiplication, rooting Induction and transplantation of tissue culture seedlings. The method of the invention induces a germination rate of 98%, the proliferation of adventitious buds of the first generation single plant is more than 15 times, and the rooting rate reaches 99%, and the method can effectively solve the problem of in vitro rapid propagation of Lycoris amaryllis.

Description

technical field [0001] The present invention relates to changing Jinhua Lycoris ( Lycoris sprengeri Comes ex Baker) tissue culture propagation method, which belongs to the technical field of seedling propagation of herbaceous ornamental plants. Background technique [0002] Replace Jinhua Lycoris ( Lycoris sprengeri Comes ex Baker) is a species of Amaryllidaceae. Perennial herb. Underground stems are thick. The bulb is ovoid, about 3.5 cm in diameter. Leaves emerge in early spring, with banded leaves. The flower stem is about 60 cm high; there are 2 involucral bracts; the umbel has 4-6 flowers; the flowers are lavender, and the top of the perianth lobes are often blue, oblanceolate, and the edges are not shrunken. The flowering period is August-September. Sexually likes a damp environment, afraid of direct sunlight, and should grow in loose and fertile sandy loam. Native to China and Japan, it is widely cultivated all over the world. It is an excellent perennial her...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 束晓春王忠张凤姣李莉庄维兵李晓丹王涛
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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