Kit for early screening of lung cancer based on peripheral blood

A kit and peripheral blood technology, applied in the field of genetic testing, can solve problems such as slow clinical development and reduce the risk of surgery

Pending Publication Date: 2019-11-15
SHENZHEN HAPLOX BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Early studies have shown circulating tumor cell (CTC) capture assays as potential biomarkers for tumor genotyping, but the technology has been relatively slow in clinical development

Method used

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  • Kit for early screening of lung cancer based on peripheral blood
  • Kit for early screening of lung cancer based on peripheral blood
  • Kit for early screening of lung cancer based on peripheral blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the composition of the kit that detects pulmonary nodule as benign or malignant

[0063] Table 1: Components of kits for detecting lung nodules as benign or malignant

[0064] Numbering name volume Specification A Streptavidin Magnetic Beads 5mL 24 times B Adapter 120μL 24 times C PCR primers 2OD 24 times D Pulmonary nodule 358 gene probe 24 μL 24 times E h 2 o

2mL

[0065] The above table 1 defines the composition of the kit for detecting pulmonary nodules as benign or malignant, and some components in the kit are further described below:

[0066] A) The streptavidin magnetic beads in the kit of the present application are used to purify the samples and PCR products by magnetic bead adsorption method treated with streptavidin. It can specifically bind to biotin at the 3' end of the probe to purify and enrich PCR products.

[0067] B) The adapter in the kit of this application is d...

Embodiment 2

[0082] Example 2: Genetic detection of pulmonary nodules in two patients (two samples are samples of benign pulmonary nodules numbered LN201601 and malignant pulmonary nodules of LN201602 respectively)

[0083] The detection method comprises the following steps:

[0084] 1. Collect blood samples from patients and separate them. The details of blood separation are as follows:

[0085] The drawn peripheral blood needs to be stored at 4°C and processed within two hours.

[0086] (1) Centrifuge at 1600g for 10min at 4°C, and divide the supernatant (plasma) into multiple 2.0mL centrifuge tubes after centrifugation. The blood cells in the lower layer are divided into 2mL cryopreservation tubes, which are the required blood cells;

[0087] (2) Centrifuge at 16,000 g for 10 min at 4°C to remove residual cells, and transfer the supernatant into a new 2.0 mL centrifuge tube to obtain the desired plasma. The samples were then stored in a -80°C freezer or dry ice for determination.

...

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Abstract

The invention discloses a kit for early screening of the lung cancer based on peripheral blood. The kit comprises a connector, a PCR primer and a capture probe. The connector comprises a Tag sequence,a Poly N sequence, a first hairpin sequence, a second hairpin sequence, a first index sequence, a first common primer combination sequence, a second common primer combination sequence and dUTP, and the first index sequence is used for distinguishing different peripheral blood samples; the PCR primer is a primer designed for 358 genes which are related to the pulmonary nodule and indirectly influence proliferation and differentiation of cells, and the PCR primer is used for being combined with the connector; the capture probe is a probe designed for the 358 genes which are related to the pulmonary nodule and indirectly influence proliferation and differentiation of the cells, and the capture probe is used for capturing a cfDNA area of plasma in the peripheral blood samples. The provided kit can be applied to early disease screening of the lung cancer, and the pulmonary nodule is diagnosed in a noninvasive and high-sensitivity mode.

Description

[0001] This application is filed on June 07, 2017 , the application number is 201710422812.9 , the name of the invention is A sort of Kit for detecting benign or malignant pulmonary nodules and application thereof divisional application of the patent application. technical field [0002] The application relates to the field of gene detection, in particular to a kit for early screening of lung cancer based on peripheral blood. Background technique [0003] Lung cancer is the malignant tumor with the highest morbidity and mortality in the world, with an annual death toll of about 1.4 million, accounting for 18% of all malignant tumor deaths. Professor Peto, a famous British oncologist, predicted that if China does not control smoking and air pollution in time, by 2025, there will be more than 1 million new lung cancer patients every year. With existing detection methods, about 75% of lung cancer patients are diagnosed at an advanced stage, and the 5-year survival rate is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6869
CPCC12Q1/6827C12Q1/6886C12Q2600/156C12Q2525/191C12Q2525/301C12Q2531/113C12Q2535/122
Inventor 张晓妮刘园园温媛
Owner SHENZHEN HAPLOX BIOTECH
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