Phase Shift Interferometric Imaging - Directed Emission Surface Enhanced Raman Spectrometer

Abstract

The embodiment of the present invention discloses a phase-shift interference imaging-directed emission surface-enhanced Raman spectrometer, which includes an optical coupling system including a coupling prism and a dielectric film for exciting long-range surface plasmons and arranged on the coupling prism, for A laser phase shifting interference imaging system that outputs laser light, and performs interference imaging on the laser light reflected back by the coupling prism and the reference light to obtain a three-dimensional phase map of the biological sample to be measured, and is used to collect the three-dimensional phase map at the same position under the action of the same laser beam The SERS signal detection system of the SERS signal generated at the place; the biological sample to be tested is set under the dielectric film, and the coupling prism is used to couple the laser light transmitted through the biological sample to be tested to the dielectric film, so as to excite the biological sample to be tested to generate SERS signal and pass through The coupling prism exits at a specific angle. The present application realizes the acquisition of molecular interaction information of biological systems from multiple channels, so as to meet the actual demand for a large number of various types of biological feature information data in related fields.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of optical instrument preparation, in particular to a phase-shift interference imaging-directed emission surface-enhanced Raman spectrometer. Background technique [0002] With the rapid development of laser technology, nanotechnology and computer technology, SERS (Surface-enhanced Raman spectroscopy, surface-enhanced Raman spectroscopy) has been used in interface and surface science, material analysis, biology, medicine, food safety, environmental monitoring and national security, etc. field has been widely used. SERS technology can provide richer structural information of chemical molecules, realize real-time and in-situ detection, and has high sensitivity, simple data processing, and high accuracy. It is a very powerful trace detection tool. [0003] For example, due to its characteristics of no need for sample pretreatment, easy operation, fast detection speed, high accuracy, a...

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Problems solved by technology

[0004] Although the SERS spectrometer can carry out a large number of biological studies as mentioned above based on the obtained rich molecular feature information, for example, in the study of the intrinsic relationship between SPs (Surface Plasmons, surface plasmons) and surface enhanced spectroscopy in biomolecular interaction systems or When it comes to the mechanism and principle of biomolecular interactions, it is not enough to rely solely on the biometric information obtained by SERS spectrometers, and more information on biomolecular interaction processes is needed

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