Medicinal reagent for treating colon cancer and preparation method thereof

A technology for colon cancer and drugs, applied in the fields of nanomaterials and biology, can solve the problems that cannot be used to improve the therapeutic effect of colon cancer, can not be used as a therapeutic drug, etc., to achieve the effect of promoting chemodynamic therapy, improving the therapeutic effect, and enhancing the effect.

Inactive Publication Date: 2019-11-29
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagent obtained by the method of this patent technology is used as an MRI contrast agent for tumor detection, but this material cannot be used as a therapeutic drug, let alone improve the therapeutic effect of colon cancer

Method used

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  • Medicinal reagent for treating colon cancer and preparation method thereof
  • Medicinal reagent for treating colon cancer and preparation method thereof
  • Medicinal reagent for treating colon cancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A preparation method for a pharmaceutical reagent for colon cancer treatment, comprising the following steps:

[0042] (1) Preparation of EA-Fe@BSA nanomaterials: 66 mg of bovine serum albumin was dissolved in 9 mL of deionized water, and 500 μL of ferric chloride solution with a concentration of 10 mg / mL was added under stirring to obtain a bovine serum albumin-Fe solution; Under stirring, 1 mL of ellagic acid solution with a concentration of 10 mg / mL was added to the bovine serum albumin-Fe solution, stirred at room temperature for 12 hours, and then purified by ultrafiltration and centrifugation to obtain the EA-Fe@BSA nanoparticle solution. The solution is a pharmaceutical agent for colon cancer treatment.

[0043] (2) Exploring the properties of EA-Fe@BSA in vitro: characterizing its particle size and morphology; photothermal and chemical kinetic properties.

[0044] (3) Based on the high concentration of hydrogen sulfide in colon cancer, Fe(III) in EA-Fe@BSA can ...

Embodiment 2

[0058] A preparation method for a pharmaceutical reagent for colon cancer treatment, comprising the following steps:

[0059] (1) Preparation of EA-Fe@BSA nanomaterials: Dissolve 20 mg of bovine serum albumin in 9 mL of deionized water, add 200 μL of ferric chloride solution under stirring to obtain bovine serum albumin-Fe solution, ferric chloride aqueous solution The concentration is 1 mg / mL; add 1 mL of ellagic acid to the bovine serum albumin-Fe solution under stirring, the concentration of ellagic acid is 2 mg / mL, and after stirring at room temperature for 12 hours, perform ultrafiltration and centrifugation for purification to obtain EA - Fe@BSA nanoparticle solution.

Embodiment 3

[0061] A preparation method for a pharmaceutical reagent for colon cancer treatment, comprising the following steps:

[0062] (1) Preparation of EA-Fe@BSA nanomaterials: Dissolve 10 mg of bovine serum albumin in 9 mL of deionized water, add 200 μL of ferric chloride solution under stirring to obtain bovine serum albumin-Fe solution and ferric chloride aqueous solution The concentration is 5 mg / mL; add 300 μL ellagic acid into the bovine serum albumin-Fe solution under stirring, the concentration of ellagic acid is 5 mg / mL, and after stirring at room temperature for 6 hours, perform ultrafiltration and centrifugation for purification to obtain EA - Fe@BSA nanoparticle solution.

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Abstract

The invention relates to a preparation method of a medicinal reagent for treating colon cancer. The preparation method comprises the following steps: dissolving bovine serum albumin in deionized waterto prepare a uniform bovine serum albumin solution; adding an aqueous solution of a soluble trivalent iron salt into the bovine serum albumin solution under a stirring condition to obtain a bovine serum albumin-Fe solution; adding an ellagic acid solution into the bovine serum albumin-Fe solution under a stirring condition to prepare an ellagic acid-Fe-bovine serum albumin solution; and ultrafiltering and centrifuging the ellagic acid-Fe-bovine serum albumin (BSA) solution for purification to obtain an ellagic acid-Fe-BSA nanoparticle solution which is the medicinal reagent for treating coloncancer. Compared with the prior art, the medicinal reagent provided by the invention has a good treatment effect on colon cancer and can be used for enhancing chemical kinetics-photothermal therapy-chemotherapy combined treatment.

Description

technical field [0001] The invention relates to the fields of nanomaterials and biotechnology, in particular to a pharmaceutical agent for treating colon cancer and a preparation method thereof. Background technique [0002] Malignant tumors have become a serious disease that seriously endangers human life and health and restricts social and economic development. Colon cancer is one of the most common cancers in the world. The early symptoms are not obvious, but in the late stage, it shows systemic symptoms such as anemia and weight loss. Its morbidity and mortality are high. By far the most common clinical diagnosis is colonoscopy. However, according to relevant statistics, the misdiagnosis rate of rectal cancer is 30%. Treatment for rectal cancer is surgery and radiation therapy. However, performing surgical radiation therapy suffers from recurrence, metastasis, and great pain. Therefore, there is an urgent need to develop more effective diagnostics and minimally inva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/366A61K33/26A61K9/51A61K47/42A61P35/00
CPCA61K9/5169A61K31/366A61K33/26A61K41/0052A61P35/00A61K2300/00
Inventor 安璐田清晴田启威杨仕平
Owner SHANGHAI NORMAL UNIVERSITY
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