A kind of extracting method and application of active substance of macalepro
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A technology of active substances and uses, which is applied in the field of extraction of macalepro active substances, and can solve problems such as unclear specific functions of the extracts
Active Publication Date: 2021-07-30
湖南医药学院
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Problems solved by technology
At present, there are few studies on the active substances in macalepro, and the specific function of the extract is not clear
Method used
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Embodiment 1
[0023] Macale C medicinal material, pulverized, crossed No. 3 sieves, weighed 200g, heated and refluxed for 3 times, each time 95% ethanol solution of 6 times the amount was added, refluxed for 1 hour, combined with the subsequent filtrate, recovered to dryness under reduced pressure , to obtain, ethanol extract (X), take part of the ethanol extract, add appropriate amount of water to dissolve, use 2 / 3 times the volume of petroleum ether, ethyl acetate, and n-butanol to extract 5 times respectively, and recover petroleum ether under reduced pressure , ethyl acetate, n-butanol, and the remaining water liquid to obtain petroleum ether part (III), ethyl acetate part (IV), n-butanol part (V) and water part (VI).
Embodiment 2
[0025] Carry out functional detection to the extract in embodiment 1, specific method is as follows:
[0026] 1.1 MTT method to detect cell proliferation
[0027] The MC3T3-E1 cells in the logarithmic phase were digested with trypsin and prepared into a single cell suspension. After counting, 5×103 cells were seeded in a 96-well plate, and cultured overnight until the cells adhered to the wall and grew well. The drug treatment time was 24h, 48h and 72h respectively. After the treatment, 20ul of MTT with a concentration of 5mg / ml was added to each well. After continuing to cultivate for 4h, the supernatant was removed, and 100ul of DMSO was added to each well to dissolve the formed purple formazan. After mixing, the absorbance at 490 nm was detected with a microplate reader.
[0029] The MC3T3-E1 cells in the logarithmic phase were digested with trypsin and prepared into a single cell suspension. After counting, 1...
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Abstract
The invention discloses a method for extracting active substances of macale, which comprises the following steps: taking macale as a medical material, pulverizing, heating and refluxing with ethanol solution to extract and then filtering to obtain a filtrate, and recovering the filtrate to dryness under reduced pressure to obtain alcohol extraction substance (X), take the alcohol extract (X) and dissolve it in water to obtain a solution, and the solution is extracted with petroleum ether, ethyl acetate, and n-butanol successively, and the petroleum ether, ethyl acetate, n-butanol and remaining water are recovered under reduced pressure. Liquid, that is, the oil ether part (III), the ethyl acetate part (IV), the n-butanol part (V) and the water part (VI). The invention provides a method for extracting the active substance of macalepro, and tests are carried out on the use of each extract on bone cells, and the use of different extracts of macalepro is clarified. Medicines for diseases provide the basis.
Description
Technical field: [0001] The present invention relates to a medicine extraction method and application, in particular to a macalepro active substance extraction method and application thereof. Background technique: [0002] Macalep is a special medicine for bruises that has been commonly used in Dong medicine from ancient times to the present. It is commonly known as Mao Xiucai in Chinese. , Produced in western Sichuan, northern to southern Yunnan, western Guizhou, western Guangxi, mostly in low mountain areas at an altitude of 1200-2100 under miscellaneous forests, grass slopes and wet oil grasslands. It is also distributed in Vietnam, Myanmar, Thailand, India, Bhutan and Nepal. It can eliminate wind and cold, dredge meridians, eliminate accumulation and relieve pain. Its nature is flat, ignorant and bitter. At present, there are few studies on the active substances in macalepro, and the specific function of the extract is not clear. Invention content: [0003] The obje...
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