Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for polyclonal antibody of specific recognition protein PRM2 and application of preparation method

A polyclonal antibody and protein recognition technology, applied in the direction of serum immunoglobulin, anti-animal/human immunoglobulin, material inspection products, etc., can solve problems such as being in the blank stage, and achieve easy access, high expression yield, Physical fitness effect

Inactive Publication Date: 2019-12-03
NANJING MEDICAL UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the relevant research on the preparation method of protein PRM2 polyclonal antibody in the prior art is still in a blank stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for polyclonal antibody of specific recognition protein PRM2 and application of preparation method
  • Preparation method for polyclonal antibody of specific recognition protein PRM2 and application of preparation method
  • Preparation method for polyclonal antibody of specific recognition protein PRM2 and application of preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] The polyclonal antibody is a mouse IgG2a subtype polyclonal antibody.

[0027] A method for preparing a polyclonal antibody that specifically recognizes protein PRM2, comprising the steps of:

[0028] (1) Obtain the PRM2 protein fragment; select the target fragment by antigenic epitope analysis for recombinant expression of protein PRM2, the gene sequence of the target fragment is shown in SEQ ID No.1 in the sequence table, and its corresponding amino acid sequence is shown in sequence As shown in SEQ ID No.4 in the list, BamH1, NotI restriction sites and homology arms were introduced into the upstream and downstream primers respectively;

[0029] An upstream primer, the specific sequence of which is shown in SEQ ID No.2 in the sequence listing;

[0030] A downstream primer, the specific sequence of which is shown in SEQ ID No.3 in the sequence listing;

[0031] (2) extract human normal kidney tissue mRNA, and use it as a template to perform reverse transcription PCR,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biological science and detection reagents, in particular to a preparation method for a polyclonal antibody of specific recognition protein PRM2 and application of the preparation method. The preparation method comprises the following steps that (1) antigen epitope analysis of the protein PRM2 is conducted, specifically, a target fragment is selectedthrough antigen epitope analysis as recombinant expression of the protein PRM2, a gene sequence of the target fragment is shown in the SEQ ID NO.1 in a sequence list, and BamH1 and Not I digestion sites are correspondingly introduced into an upstream primer and a downstream primer. According to the preparation method, the preparation of the polyclonal antibody of the PRM2 is achieved.

Description

technical field [0001] The invention relates to the technical fields of biological science and detection reagents. Background technique [0002] The problem of infertility has always been an important topic in the research of human reproduction. Now 15% of couples in the world suffer from this problem, about half of which are caused by the man. The study of its mechanism is essential for finding solutions to infertility. [0003] During the haploid stage of vertebrate spermatogenesis, protamine replaces histones in sperm chromatin and becomes the major DNA-binding protein in the sperm nucleus. With the help of protamine, the DNA double strand undergoes a series of coiling, folding, and compression to form high-density chromosomes, whose volume is less than 5% of the somatic nucleus. Most mammals have only one type of protamine (protamine 1); however, some species, including humans and mice, have two types of protamine: protamine 1 and protamine 2 (PRM2, protamine 2). Stu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C07K16/06G01N33/68
CPCC07K16/06C07K16/18G01N33/689G01N2333/47
Inventor 刘明兮刘思钰吴佳艳王莹
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com