Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of polyclonal antibody for specifically recognizing protein USP42

A polyclonal antibody and protein recognition technology, applied in biochemical equipment and methods, anti-enzyme immunoglobulin, and the use of vectors to introduce foreign genetic material, etc., to achieve the effects of high expression yield, robust physique, and easy access

Inactive Publication Date: 2019-12-03
NANJING MEDICAL UNIV
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, the relevant research on the preparation method of the protein USP42 polyclonal antibody is still in a blank stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of polyclonal antibody for specifically recognizing protein USP42
  • Preparation method and application of polyclonal antibody for specifically recognizing protein USP42
  • Preparation method and application of polyclonal antibody for specifically recognizing protein USP42

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] The polyclonal antibody is a mouse IgG2a subtype polyclonal antibody.

[0033] The preparation method of the above-mentioned polyclonal antibody specifically recognizing protein USP42 comprises the following steps:

[0034] (1) Obtain the USP42 protein fragment; select the target fragment by antigenic epitope analysis for recombinant expression of protein USP42, the gene sequence of the target fragment is shown in SEQ ID No.1 in the sequence listing, and its corresponding amino acid sequence is shown in sequence As shown in SEQ ID No.4 in the list, BamH1, NotI restriction sites and homology arms were introduced into the upstream and downstream primers respectively;

[0035] An upstream primer, the specific sequence of which is shown in SEQ ID No.2 in the sequence listing;

[0036] A downstream primer, the specific sequence of which is shown in SEQ ID No.3 in the sequence listing;

[0037] (2) extract the normal testicular tissue mRNA of the mouse, and use it as a temp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of bioscience and detection reagents. The invention specifically discloses a preparation method and application of a polyclonal antibody for specifically recognizing protein USP42. The method comprises the following steps: (1), protein USP42 antigenic epitope analysis: selecting a target fragment as recombinant expression of protein USP42 through the antigenic epitope analysis, wherein the gene sequence of the target fragment is shown as SEQ ID N0.1 in a sequence table; and respectively introducing BamH1 and Not I restriction enzyme cutting sites into an upstream primer and a downstream primer. According to the invention, the preparation of the polyclonal antibody of USP42 is realized.

Description

technical field [0001] The invention relates to the technical fields of biological science and detection reagents. Background technique [0002] Deubiquitinating enzyme USP42 (Ubiquitin specific peptidase 42) is a member of the USP family of deubiquitinating enzymes (DUBs). Ubiquitination is the labeling effect of ubiquitin, which is catalyzed by three enzymes. Ubiquitin is a protein composed of 76 amino acids that is widely found in eukaryotic cells. The protein degradation pathway mediated by ubiquitin is called "ubiquitin-proteasome pathway". The protein to be degraded is released as a degradation product after unfolding, opening, and hydrolysis. Ubiquitination is a reversible process. Ubiquitination is a cascade of enzymes that cause ubiquitin to mark selected proteins. Deubiquitination separates ubiquitin from proteins by deubiquitinases. The processes of deubiquitination and ubiquitination constitute protein homeostasis in vivo. At present, research on deubiquitin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/40C12N15/70G01N33/573C12N9/48
CPCC07K16/40C12N9/485C12N15/70C12Y304/19012G01N33/573G01N2333/948
Inventor 刘明兮刘思钰吴佳艳
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com