Bladder cancer detection kit and its application

A detection kit, bladder cancer technology, applied in the field of bladder cancer detection kits, to achieve the effects of high stability, automatic result judgment, and high sensitivity

Active Publication Date: 2022-06-24
CANCER CENT OF GUANGZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, domestic patents on exosomes mainly focus on the separation and detection of exosomes, but there are few public reports on the use of exosomes extracted from urine for auxiliary diagnosis of bladder cancer. Only Zhejiang University has published a urine exosome An integrated detection method and detection chip for body separation, enrichment and detection. By detecting cancer information in urine exosomes, it is expected to be used for non-invasive diagnosis and monitoring of bladder cancer. This method uses the unique transmembrane protein CD63 of exosomes A rapid ELISA method for the diagnosis of bladder cancer was established, but the screening of bladder cancer-specific tumor markers was not carried out

Method used

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  • Bladder cancer detection kit and its application
  • Bladder cancer detection kit and its application
  • Bladder cancer detection kit and its application

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Effect test

Embodiment 1

[0062] 1 Urine sample collection and preprocessing: 125 bladder cancer patients (patients before treatment) and 146 controls (including 50 healthy people and 96 patients with benign urinary system diseases) urine samples, a total of 271 urine samples. The urine is morning urine or urine held for more than 2 hours, more than 15ml. After collection, it should be sent for inspection in time, and the storage time at room temperature should not exceed 2 hours. The urine samples were centrifuged at 2000g for 10min, and the supernatant was transferred to a new centrifuge tube for exosome extraction. Urine exfoliative cytology was performed on the sediment with Wright's staining.

[0063] 2. Isolation of urinary exosomes: The exosomes were isolated by PEG precipitation. 15-20ml urine supernatant, 3000g, centrifuge at 4 degrees for 15min, take the supernatant to another new centrifuge tube, add PEG8000 with a final concentration of 8%, incubate overnight at 4 degrees after mixing, an...

Embodiment 2

[0085] Example 2 Evaluation of the diagnostic performance of four miRNAs

[0086]The diagnostic ability of the four serum miRNAs to discriminate between bladder cancer patients and controls was compared by ROC curve analysis. The area under the ROC curve (AUC) of the four miRNAs miR-423-5p, miR-203-3p, miR-21-5p and miR-30b-5p were: 0.880 (95%CI 0.836-0.917), 0.703 (95%) CI 0.645-0.757), 0.799 (95% CI 0.746-0.845), 0.850 (95% CI 0.802-0.890), see figure 2 . miR-423-5p sensitivity 69.9%, specificity 92.8%; miR-203-3p sensitivity 63.7%, specificity 76.0%; miR-21-5p sensitivity 65.8%, specificity 91.2%; miR-30b-5p sensitivity 69.9% , the specificity was 86.4%. For more accurate prediction of bladder cancer, a combination of biomarkers is a better choice. Logistic regression and neuron algorithm were used to establish joint diagnosis model. The combined sensitivity and specificity of 4 miRNA markers (miR-423-5p, miR-203-3p, miR-21-5p, miR-30b-5p) can reach 81.5% and 91.2%, t...

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Abstract

The present invention provides a bladder cancer detection kit and its application, by detecting at least one of miR-423-5p, miR-203-3p, miR-21-5p, and miR-30b-5p in tumor-derived exosomes To provide a kit and system that can detect tumors using urine as a biological sample, with high stability and accurate quantification. It is a fast, low-cost, high-accuracy, and high-sensitivity kit .

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a bladder cancer detection kit and application thereof. Background technique [0002] Bladder cancer refers to the most common malignant tumor of the urinary system that occurs on the bladder mucosa. The average age of onset is about 65 years old. About 90% of them are transitional cell carcinomas, and the other 10% are mainly squamous cell carcinomas and adenocarcinomas. The incidence rate of bladder cancer in the national tumor registration area is 6.61 / 100,000, ranking the ninth in the incidence of malignant tumors. Due to the obvious characteristics of poor prognosis and easy recurrence, and the 5-year survival rate of bladder cancer patients with early detection and early treatment is greater than 90%, early diagnosis is of great significance for the treatment of this disease. Cystoscopy is currently the most reliable method for diagnosing bladder cancer. Cysto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/6886C12Q1/686C12Q2600/178C12Q2563/107C12Q2545/114
Inventor 邓敏刘万里
Owner CANCER CENT OF GUANGZHOU MEDICAL UNIV
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