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A kind of banana fruit starch branching enzyme gene masbe2.3 and its encoded protein and application

A technology for bananas and fruits, applied in application, genetic engineering, plant genetic improvement and other directions, can solve problems such as no relevant reports, and achieve the effects of increasing crop yield, improving starch quality, and increasing fructose content

Active Publication Date: 2021-02-02
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although studies on SBEI or SBEII have been carried out in a variety of plants, there are no relevant reports on fresh starch-converted fruits, such as bananas, kiwifruit, mango, etc.

Method used

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  • A kind of banana fruit starch branching enzyme gene masbe2.3 and its encoded protein and application
  • A kind of banana fruit starch branching enzyme gene masbe2.3 and its encoded protein and application
  • A kind of banana fruit starch branching enzyme gene masbe2.3 and its encoded protein and application

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Embodiment Construction

[0021] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] 1. Gene acquisition

[0023] Using the cDNA of banana fruit as a template, the

[0024] 5'-ATGGCCTTCACCCTCGCGGGGATC-3'

[0025] 5'-TCAGTCCACAGAAAGAGCATAAAC-3'

[0026] As a primer, a base sequence obtained by PCR amplification is 2,502bp ( figure 1 ), its sequence is shown in SEQ ID No.1; the amino acid sequence of the gene encoding MaSBE2.3 is shown in SEQ ID No.2.

[0027] 2. Construction of recombinant vector

[0028] 1. pCAMBIA1304-MaSBE2.3 recombinant plasmid

[0029] With the nucleotide sequence of the above-mentioned banana fruit starch branching enzyme gene MaSBE2.3, two kinds of restriction endonucleases, Bgl I and Spe I, were ...

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Abstract

The invention provides a banana fruit starch branching enzyme gene MaSBE2.3. The nucleotide sequence of the banana fruit starch branching enzyme gene MaSBE2.3 is shown in SEQ ID NO:1. The invention further provides an encoded protein and application of the banana fruit starch branching enzyme gene MaSBE2.3. The banana fruit starch branching enzyme gene MaSBE2.3 can improve the quality of fruit amylopectin and increase the content of glucose and fructose of fruits, for example, the banana fruit starch branching enzyme gene MaSBE2.3 is introduced into the banana fruits, the gene MaSBE2.3 is overexpressed, accordingly, the activity of a starch branching enzyme (SBE) is improved, and the expression quantity of the MaSBE2.3 and the content of the amylopectin are increased; and therefore, the banana fruit starch branching enzyme gene MaSBE2.3 has important significance for regulating the content of the amylopectin, or increasing the yield of crops, improving the quality of the amylopectin and culturing high-quality new banana species, and a gene resource candidate is provided for improving the quality of the amylopectin of bananas or other fruits.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a banana fruit starch branching enzyme gene MaSBE2.3 and its encoded protein and application. Background technique [0002] The amylopectin content of banana fruit directly affects its yield, quality and processing performance. The study of amylopectin synthase gene is the basis for increasing yield, improving quality and meeting the diverse needs of starch processing. Starch branching enzyme (SBE) is the key enzyme for the synthesis of amylopectin in the starch body, which can cut the α-1,4-D-glucan straight chain donor (amylose or amylopectin straight chain region) α-1,4-glycosidic bond, and at the same time catalyze the formation of α-1,6-glycosidic bond between the cut short chain and the acceptor chain (original chain or other chain), thereby generating branching. [0003] SBE mainly includes two branching enzymes, SBEI and SBEII. In monocots, the SBEII gene exists ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82C12N15/11
CPCC12N9/107C12N15/8245C12Y204/01018Y02A40/146
Inventor 苗红霞金志强徐碧玉刘菊华孙佩光贾彩红张建斌王卓王静毅
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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