Medical dressing containing human fibroblast regulated culture solution, preparation method and application

A technology of human fibroblasts and culture medium, applied in the field of medical devices, can solve problems such as insignificant effects, achieve the effects of no toxic and side effects, improve bioavailability, and promote cell migration

Active Publication Date: 2021-12-28
西安艾尔菲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The third object of the present invention is to provide the application of the above-mentioned medical dressing in hormone-dependent dermatitis, and solve the problem that the effect of existing dressing products is not obvious

Method used

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  • Medical dressing containing human fibroblast regulated culture solution, preparation method and application
  • Medical dressing containing human fibroblast regulated culture solution, preparation method and application
  • Medical dressing containing human fibroblast regulated culture solution, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] A medical dressing containing human fibroblast-regulated culture fluid, the preparation method of which is as follows:

[0077] Step 1, the preparation of liposome freeze-dried powder A of encapsulating human fibroblasts to adjust the culture medium:

[0078] Step 1.1, human fibroblast-conditioned medium concentrated by ultrafiltration:

[0079] Concentrate the conditioned human fibroblast culture solution by ultrafiltration, and filter the filtrate through a 0.22 μm sterile filter to obtain a conditioned human fibroblast culture concentrated solution, the concentration of which is 10 mg / mL.

[0080] Step 1.2, preparation of liposome suspension wrapped in human fibroblast conditioned medium:

[0081] Dissolve 15.0g of soybean lecithin, 5.0g of cholesterol, and 1.0g of vitamin E in 63mL of absolute ethanol, and remove the absolute ethanol by rotary evaporation under reduced pressure at 0.04Mpa and 35°C to obtain a uniform film; take step 1.1 to obtain Add 20 mL of 10 m...

Embodiment 2

[0089] A medical dressing containing human fibroblast-regulated culture fluid, the preparation method of which is as follows:

[0090] Step 1, preparation of human fibroblast regulated culture medium liposome lyophilized powder A:

[0091]Step 1.1, human fibroblast-conditioned medium concentrated by ultrafiltration:

[0092] Concentrate the conditioned human fibroblast culture solution by ultrafiltration, and filter the filtrate through a 0.22 μm sterile filter to obtain a conditioned human fibroblast culture concentrated solution with a concentration of 15 mg / mL.

[0093] Step 1.2, preparation of liposome suspension wrapped in human fibroblast conditioned medium:

[0094] Dissolve 12g of soybean lecithin, 4g of cholesterol, and 0.5g of vitamin E in 64mL of absolute ethanol, and remove the absolute ethanol by rotary evaporation under reduced pressure at 0.05Mpa and 37°C to obtain a uniform film; take 15mg prepared in step 1.1 / mL human fibroblast adjusted culture concentrate...

Embodiment 3

[0102] A medical dressing containing human fibroblast-regulated culture fluid, the preparation method of which is as follows:

[0103] Step 1, preparation of human fibroblast regulated culture medium liposome lyophilized powder A:

[0104] Step 1.1, human fibroblast-conditioned medium concentrated by ultrafiltration:

[0105] Concentrate the conditioned human fibroblast culture solution by ultrafiltration, and filter the filtrate through a 0.22 μm sterile filter to obtain a conditioned human fibroblast culture concentrated solution, the concentration of which is 20 mg / mL.

[0106] Step 1.2, preparation of liposome suspension wrapped in human fibroblast conditioned medium:

[0107] Dissolve 3.0g of soybean lecithin, 1.0g of cholesterol, and 3.0g of vitamin E in 35ml of absolute ethanol, and remove the absolute ethanol by rotary evaporation under reduced pressure at 0.06Mpa and 40°C to obtain a uniform film; take step 1.1 to obtain Add 25mL of 20mg / mL human fibroblast conditio...

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Abstract

The invention discloses a medical dressing plaster containing human fibroblast regulated culture fluid. The material liquid part is composed of the following components according to the mass percentage, which wraps human fibroblast regulated culture fluid liposome freeze-dried powder A 1-8% , mixed solution B 92-99%, the mass percentage of each of the above components is 100%, and parts A and B are packaged independently. The medical dressing of the present invention combines the liposome freeze-dried powder of the human fibroblast-adjusted culture solution with the mixed solution, and is applied to wound repair, which is beneficial to the absorption of active ingredients in the culture solution, and enhances the transdermal absorption capacity of cytokines and collagen , increased the bioavailability of cytokines in human fibroblast-conditioned medium. In addition, sodium hyaluronate can promote cell migration, differentiation and wound healing. It has high viscosity elasticity, plasticity and good biocompatibility. The combination of the two is beneficial to the repair of skin damage.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a medical dressing containing human fibroblast regulated culture solution, the invention also relates to a preparation method of the medical dressing, and the application of the above medical dressing. Background technique [0002] With the development of our country's society and economy, the medical device market is growing day by day, and the repair of facial trauma has become a topic that people are more and more concerned about. Various hyaluronic acid and collagen-based medical dressings are constantly being used in facial beauty and trauma treatment. . The process of wound repair is not only the process of cell proliferation, differentiation, migration, apoptosis, and disappearance, but also the result of a series of network interactions between different types of cells, structural proteins, and growth factors. Studies have confirmed that growth factor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/40A61L15/20A61L15/28A61L15/44
CPCA61L15/40A61L15/20A61L15/28A61L15/44A61L2300/30A61L2300/412
Inventor 肖萍王海江王静胡春梅
Owner 西安艾尔菲生物科技有限公司
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