Biological photosensitive resin, preparation method thereof and method for constructing three-dimensional pre-vascular network channel
A bio-light and resin technology, applied in opto-mechanical equipment, photosensitive materials for opto-mechanical equipment, optics, etc., can solve the problems of low efficiency, lack of efficient construction of complex three-dimensional pre-vascular network channels, poor mechanical properties, etc.
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[0034] In the present invention, the preparation method of the methacrylated gelatin preferably comprises the following steps:
[0035] After mixing pigskin gelatin, methacrylic anhydride and phosphate buffer solution, modification reaction is carried out to obtain methacrylated gelatin.
[0036] In the present invention, the pigskin gelatin, methacrylic anhydride (MA) and phosphate buffered saline (PBS) are preferably mixed with pigskin gelatin and phosphate buffered saline, and stirred at 55-65°C until completely Dissolving to obtain a pigskin gelatin solution, and then adding methacrylic anhydride to the pigskin gelatin solution; the adding rate of the methacrylic anhydride is preferably 0.4-0.6 mL / min, more preferably 0.5 mL / min. In the present invention, the dosage ratio of the phosphate buffer solution to pigskin gelatin is preferably (90-110) mL: 10 g, more preferably 100 mL: 10 g; the phosphate buffer solution is used as a solvent to ensure the modification reaction w...
Embodiment 1
[0054] (1) Mix 10 g of pigskin gelatin with phosphate buffered saline (PBS), stir until completely dissolved at 60° C. to obtain a pigskin gelatin solution, and the concentration of pigskin gelatin is 0.1 g / mL; Add methacrylic anhydride (MA) in the solution at a rate of 0.5mL / min, so that the volume concentration of MA in the resulting mixed material liquid is 8%, then stir and react at 50°C for 1h; after the reaction is completed, use a temperature of 30 Dilute the obtained system to twice the original volume with PBS at ℃ to terminate the reaction, and then use a dialysis bag with a molecular weight cut-off of 10kDa to dialyze at 40℃ for 7 days, and the dialysate is changed once a day to remove unreacted MA and other impurities , to obtain a methacrylated gelatin (GelMA) solution; the GelMA solution was pre-frozen at -50°C for 12 hours to form ice cubes, and the ice cubes were placed in a freeze dryer at -80°C Freeze-drying under the same conditions for 72 hours to obtain Ge...
Embodiment 2
[0062] (1) Prepare GelMA powder according to the method of Example 1, mix the GelMA powder with deionized water, and stir at 60°C until completely dissolved to obtain a GelMA feed solution, the concentration of GelMA is 0.15g / mL; Diurethane dimethacrylate (UDMA) and hydroxyapatite (HAP) are added in the described GelMA feed liquid, the concentration of control UDMA and HAP is respectively 0.1g / mL and 0.3g / mL, then at 60 Shake and stir at ℃ until completely dissolved to obtain a mixed dispersion;
[0063] The mixed dispersion liquid is mixed with photoinitiator Irgacure 2959 and HMBS ultraviolet retardant, and fully stirred and mixed evenly in a light-proof environment to obtain a biophotosensitive resin; wherein, the concentration of the photoinitiator in the biophotosensitive resin is 0.08g / mL, the concentration of HMBS ultraviolet blocker in the biophotosensitive resin is 0.001g / mL;
[0064] (2) use the modeling software SolidWorks modeling according to the method for embod...
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