Novel excellent flower spring blood wood tissue culture rapid propagation method

A technology for tissue culture and rapid propagation of flowers, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as the length of internodes of subcultured materials, the impact of transplanting survival rate, and callus at the base of stems, etc., to achieve Save import cost, fast elongation and growth of adventitious buds, and thick cluster buds

Inactive Publication Date: 2019-12-17
海南省林业科学研究所
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gao Liqiong et al. and Yang Yan et al. successfully established the regeneration system of Eucalyptus red crown, but there are the following problems: ① The internodes of the subculture propagation materials are long, and the average internode length of different clones is 1.6-2.4 cm, which is easy to cause proliferation During the process of inducing rooting, the material is prone to stem death and weak seedlings; ②Callus tissue is produced at the base of the stem during the rooting process, which is also greatly affected in the later transplanting survival rate; ③Long rooting time, long up to about 7 weeks

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] A new and excellent flower red crown eucalyptus tissue culture rapid propagation method, comprising the following steps:

[0031] C1: Explant Pretreatment

[0032] Cut off the branches of the perennial red crown eucalyptus, and after 45 days of sprouting and growth of the new shoots at the pruned part, collect the semi-lignified new shoots, cut off the stem section of the new shoots that is 5 cm long and has a bud below the stem tip of the new shoots, and use them as explants for later use ;

[0033] C2: Surface disinfection of explants

[0034] After washing the explants in step C1 with tap water for 15 minutes, soak them in sterile water for 1 minute in a sterile ultra-clean bench, sterilize them with 10% sodium hypochlorite solution for 5 minutes, and rinse them with sterile water for 3 to 4 times. % mercuric chloride solution for 4 to 5 minutes and rinsed with sterile water for 4 to 5 times to obtain the sterilized explants;

[0035] C3: Bud induction

[0036] I...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of plant tissue culture, and discloses a novel excellent flower spring blood wood tissue culture rapid propagation method. The method comprises the steps of explant pretreatment, explant surface disinfection, bud induction, acquisition of tufty adventitious buds, rooting culture and seedling hardening and transplanting. The method has the advantages ofrapid axillary bud induction, a high induction rate, a large proliferation multiple, thick and strong tufty buds, rapid elongation and growth of adventitious buds, a high rooting rate, a developed root system, a thick and strong rooting seedling, a high transplanting survival rate, healthy and strong seedling growth, low planting management cost and the like, a stable and efficient spring blood wood branch stem vitro plant regeneration method is also provided, the cultured spring blood wood seedling is suitable for large-scale production, healthy and strong spring blood wood with unfolded leaves can be cultured, the import cost of spring blood wood seeds is reduced, and the significance for improving the ornamental value of the spring blood wood is important.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of new excellent flower Eucalyptus red crown. Background technique [0002] Red Crown Eucalyptus (Corymbia ptychocarpa Subsp.aptycha), English name spring blood wood, also known as wrinkled fruit Eucalyptus, belongs to Myrtaceae Eucalyptus, native to Australia. Red Crown Eucalyptus is a medium-sized tree with a height of 8-10m. The flowering period of the saplings is 3-4 years, and the flowering period is from September to March of the next year. The flower color is white, pink and deep red, and pink is common. There are many single flowers similar to torches on the flower stem. The diameter of the flower can reach 7cm, and it is located at the top of the side branch. If there is no external force shaking during the withering period, the petals of the dried flower will fall slowly, and the color of the flower is no di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邢文婷陈彧杨众养陈毅青徐佩玲董晓娜陈培陈显臻
Owner 海南省林业科学研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap