Novel excellent flower spring blood wood tissue culture rapid propagation method

Abstract

The invention relates to the technical field of plant tissue culture, and discloses a novel excellent flower spring blood wood tissue culture rapid propagation method. The method comprises the steps of explant pretreatment, explant surface disinfection, bud induction, acquisition of tufty adventitious buds, rooting culture and seedling hardening and transplanting. The method has the advantages ofrapid axillary bud induction, a high induction rate, a large proliferation multiple, thick and strong tufty buds, rapid elongation and growth of adventitious buds, a high rooting rate, a developed root system, a thick and strong rooting seedling, a high transplanting survival rate, healthy and strong seedling growth, low planting management cost and the like, a stable and efficient spring blood wood branch stem vitro plant regeneration method is also provided, the cultured spring blood wood seedling is suitable for large-scale production, healthy and strong spring blood wood with unfolded leaves can be cultured, the import cost of spring blood wood seeds is reduced, and the significance for improving the ornamental value of the spring blood wood is important.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of new excellent flower Eucalyptus red crown. Background technique [0002] Red Crown Eucalyptus (Corymbia ptychocarpa Subsp.aptycha), English name spring blood wood, also known as wrinkled fruit Eucalyptus, belongs to Myrtaceae Eucalyptus, native to Australia. Red Crown Eucalyptus is a medium-sized tree with a height of 8-10m. The flowering period of the saplings is 3-4 years, and the flowering period is from September to March of the next year. The flower color is white, pink and deep red, and pink is common. There are many single flowers similar to torches on the flower stem. The diameter of the flower can reach 7cm, and it is located at the top of the side branch. If there is no external force shaking during the withering period, the petals of the dried flower will fall slowly, and the color of the flower is no di...

AI Technical Summary

Problems solved by technology

Gao Liqiong et al. and Yang Yan et al. successfully established the regeneration system of Eucalyptus red crown, but there are the following problems: ① The internodes of the subculture propagation materials are long, and the average internode length of different clones is 1.6-2.4 cm, which is easy to cause proliferation During the process of inducing rooting, the material is prone to stem death and weak seedlings; ②Callus tissue is produced at the base of the stem during the rooting process, which is also greatly affected in the later transplanting survival rate; ③Long rooting time, long up to about 7 weeks