Application of fibroblast growth factor receptor 1 to preparation of medicines for preventing and treating enterovirus 71 type infection

A growth factor receptor, fibroblast technology, applied in the field of biomedicine

Active Publication Date: 2019-12-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there are no reports on the role of IL4R and FGFR1 molecules in enteroviruses, especially EV71-infected host cells. In-depth research on these two molecules w

Method used

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  • Application of fibroblast growth factor receptor 1 to preparation of medicines for preventing and treating enterovirus 71 type infection
  • Application of fibroblast growth factor receptor 1 to preparation of medicines for preventing and treating enterovirus 71 type infection
  • Application of fibroblast growth factor receptor 1 to preparation of medicines for preventing and treating enterovirus 71 type infection

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Embodiment 1

[0062] 1 Design and synthesis of specific siRNA sequences for each transport-related membrane protein.

[0063] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis on AXIIR, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each ge...

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Abstract

The invention relates to the technical field of biomedical science, in particular to a new target for resisting enterovirus 71 type infection, and an application. Human colon cancer cells (Caco-2) areused as target cells, an RNA interference technique is adopted for reducing expression of a target cell membrane receptor, a host factor which can effectively restrain EV71 infected human colon cancer cells is sought, and the purpose of blocking EV71 infection from a source (intestinal tract) is achieved. Through experiment, the inventor of the invention finds that the fibroblast growth factor receptor 1 (FGFR1) exerts important effects in EV71 infected Caco-2 cells, so that when the expression of the FGFR1 is reduced, the EV71 infection can be obviously restrained. The invention provides anapplication of the FGFR1 to preparation of medicines for preventing or treating enterovirus 71 type infection, and a new target and a treatment scheme are provided for clinical prevention and treatment of diseases of hand-foot-mouth diseases, meningitis, brainstem encephalitis or poliomyelitis and the like caused by the EV71 infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular, a new target and application for resisting enterovirus 71 infection. Background technique [0002] Enterovirus 71 (enterovirus 71, EV71) belongs to the picornaviridae enterovirus and belongs to human enterovirus A, and is one of the main pathogens causing hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms on the hands, feet, buttocks and oral mucosa; a small number of children can develop into severe patients with central nervous system (central nervous system , CNS) lesions, including aseptic meningitis, brainstem encephalitis, encephalomyelitis, and neurogenic pulmonary edema, which seriously threaten the life and heal...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P31/14
CPCA61K45/00A61P31/14
Inventor 朱勇喆陈洋张展榕刘延刚赵平戚中田
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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