Method for constructing immune healthy synovial sarcoma xenograft mouse model

A technique of synovial sarcoma and xenotransplantation, which is applied in the field of establishment of tumor transplantation mouse models, can solve the problems of inconvenient experiments and impossibility of passage studies, and achieve the effect of broadening the scope of application and improving the effect of non-immune system

Active Publication Date: 2020-01-03
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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Problems solved by technology

However, this method is mainly due to the fact that the immune response caused by the initial antigen stimulation of normal immune mice is generally 7 to 10 days, while the cell-mediated immune response to the allograft is obvious on the 9th to 12th day,

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  • Method for constructing immune healthy synovial sarcoma xenograft mouse model
  • Method for constructing immune healthy synovial sarcoma xenograft mouse model
  • Method for constructing immune healthy synovial sarcoma xenograft mouse model

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[0040] After being reviewed and approved by the Hospital Ethics Committee, all patients signed an informed consent form before sample collection. The collected tissues underwent histopathological examination. The tumor tissue blocks of patients with synovial sarcoma confirmed by pathology were stored in sterile normal saline and placed on ice. superior. The tissue blocks were divided into 3 parts, the first part was directly processed under sterile conditions (see below for details) and xenografted into immunodeficient mice; the second part was inserted into 10% DMSO (dimethyl sulfoxide), 90% FBS (fetal bovine serum) and 1% NEAA (non-essential amino acids) in DMEM (a medium containing various amino acids and glucose), gradient frozen to -80 degrees, and stored in liquid nitrogen; Part 3 put Quick-frozen in liquid nitrogen, and then stored in liquid nitrogen. Among them, the first part is used for PDX modeling, the second part is used for resuscitation and vaccination, and the...

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Abstract

The invention provides a method for constructing an immune healthy synovial sarcoma xenograft mouse model; the method comprises cultivating a generation P0, to be specific, resuscitating a tumor tissue block of a synovial sarcoma patient stored in a liquid nitrogen environment, then transplanting the tumor tissue block into an immunodeficient mouse under a sterile condition, and pretreating a micewith normal immunity by cyclosporine; culturing a generation Px, to be specific, inoculating the tumor tissue block, grown in a mouse from any of the generations P0 to Px, into the mouse pretreated with cyclosporine to form a generation Px mouse; and culturing a generation Py, to be specific, inoculating a synovial sarcoma tumor tissue block, grown in a mouse from any of the generations Px to Py-1, into the mouse with normal immunity until tumor tissues grow in the mouse, so that culturing of the generation Py is completed. The invention solves the problem that the traditional PDX (patient-derived xenograft) model has no immune system, provides an in-vivo model for the research on tumor immunotherapy, and widens the application range of the PDX model.

Description

technical field [0001] The invention relates to the field of establishment of a tumor transplantation mouse model, in particular to a construction method of an immune-competent synovial sarcoma xenotransplantation mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcomas [1]. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-...

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Application Information

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IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008A01K2267/03
Inventor 黎志宏任晓磊万璐罗煦君陈瑞奇段智曦
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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