Method for constructing immune healthy synovial sarcoma xenograft mouse model

Abstract

The invention provides a method for constructing an immune healthy synovial sarcoma xenograft mouse model; the method comprises cultivating a generation P0, to be specific, resuscitating a tumor tissue block of a synovial sarcoma patient stored in a liquid nitrogen environment, then transplanting the tumor tissue block into an immunodeficient mouse under a sterile condition, and pretreating a micewith normal immunity by cyclosporine; culturing a generation Px, to be specific, inoculating the tumor tissue block, grown in a mouse from any of the generations P0 to Px, into the mouse pretreated with cyclosporine to form a generation Px mouse; and culturing a generation Py, to be specific, inoculating a synovial sarcoma tumor tissue block, grown in a mouse from any of the generations Px to Py-1, into the mouse with normal immunity until tumor tissues grow in the mouse, so that culturing of the generation Py is completed. The invention solves the problem that the traditional PDX (patient-derived xenograft) model has no immune system, provides an in-vivo model for the research on tumor immunotherapy, and widens the application range of the PDX model.

Description

technical field [0001] The invention relates to the field of establishment of a tumor transplantation mouse model, in particular to a construction method of an immune-competent synovial sarcoma xenotransplantation mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcomas [1]. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-...

AI Technical Summary

Problems solved by technology

However, this method is mainly due to the fact that the immune response caused by the initial antigen stimulation of normal immune mice is generally 7 to 10 days, while the cell-mediated immune response to the allograft is obvious on the 9th to 12th day, so the first 6 days The tumor can proliferate within the tumor, so the main purpose of this method is still to test drug sensitivity, the tumor will still regress, and the observation of the tumor still needs the help of a microscope. unable to proceed

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