Cultivation method of stress-resistant broccoli new variety

A cultivation method and cauliflower technology, which are applied in the field of cultivating new varieties of cauliflower with stress resistance, can solve the problems of slow breeding process, cumbersome mutation screening, difficult control of the direction and nature of mutation, etc., so as to reduce the restoration of the ecosystem and avoid inheritance The effect of instability and enrichment of vegetable germplasm resources

Inactive Publication Date: 2020-01-10
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
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Problems solved by technology

Disadvantages are: (1) Low efficiency, reason: there are not many individuals with mutations, and a large amount of materials need to be processed
(2) The frequency of beneficial mutations is low, the direction and nature of mutations are difficult to control, and mutation screening is cumbersome
The disadvantages are: (1) No new gene will be produced; (2) The offspring of the hybrid will have trait separation and instability; (3) The breeding process is slow, and the process of obtaining new varieties is long and complicated
[0006] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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Embodiment Construction

[0043] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0044] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0045] A method for cultivating a stress-resistant cauliflower new variety, the steps are as follows:

[0046] (1) Using plant tissue culture technology, using different tissue parts of cauliflower as explants, under the induction of plant hormones, to establish callus regeneration systems from different sources;

[0047] ⑵Establish the cauliflower loose embryogenic callus induction system by changing the hormone level in the medium inoculated with callus and the growth environment conditions;

[0048] (3) By adding ...

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Abstract

The invention discloses a cultivation method of a stress-resistant broccoli new variety. The method includes: building callus regeneration systems of different sources; building a broccoli loose embryonic callus induction system; screening an appropriate mutagenesis method; building an efficient regeneration system, and acquiring a regeneration culture medium; acquiring a callus growth culture medium with screening pressure; screening resistant calluses; inducing regenerated buds to obtain regenerated seedlings; further breeding individual plants survived under the screening pressure, and transplanting to a natural environment to perform further selective culture to obtain ideal stress-resistant mutants so as to obtain the stress-resistant broccoli new variety. The method has the advantages a foundation is laid for the cultivation of high-quality vegetable varieties with disease resistance, drought resistance, cold resistance and the like during production, chemical fertilizer use reduction and ecological system restoration are promoted, a new approach is provided for vegetable stress-resistant breeding, vegetable seed resources are enriched, and a foundation is laid for safety vegetable production.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a method for cultivating new stress-resistant cauliflower varieties. Background technique [0002] Cauliflower, also known as cauliflower, is a widely grown vegetable variety that is universally popular for its nutrient-rich properties. With the development of society, the problem of food safety is becoming more and more serious. Therefore, there is an urgent need for new stress-resistant cauliflower varieties that can greatly reduce the use of pesticides and fertilizers. [0003] Vegetable breeding generally adopts cross-breeding. Due to the limitation of the genetic resources of the species, some new excellent traits cannot be produced. However, the efficiency of general mutation breeding is extremely low, and it is difficult to induce new good traits. Although the gene transformation technology can introduce the target gene into the vegetable genome, but because th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/06
CPCA01H1/06A01H4/001A01H4/005A01H4/008
Inventor 刘艳军许丁帆黄俊轩武春霞李建科杨静慧李双跃
Owner TIANJIN AGRICULTURE COLLEGE
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