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Polypeptide CAK18N and application thereof to promotion of regeneration and inhibition of liver apoptosis

A liver cell and liver regeneration technology, applied in the field of cell proliferation and apoptosis, can solve the problems of CAK18N liver cell regeneration and inhibition of liver cell apoptosis, and achieve the promotion of liver cell proliferation, small molecular weight, and inhibition of liver cell apoptosis Effect

Active Publication Date: 2020-01-21
LINK HEALTH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] CAK18N is a polypeptide containing 18 amino acids. It has been reported that CAK18N can inhibit angiogenesis induced by vascular epidermal growth factor (VEGF), and can be used for the treatment of diseases such as tumors and wet macular degeneration (see Chinese patent CN109593117A), but it has not been seen so far. CAK18N is used to promote liver cell regeneration and inhibit liver cell apoptosis report

Method used

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  • Polypeptide CAK18N and application thereof to promotion of regeneration and inhibition of liver apoptosis
  • Polypeptide CAK18N and application thereof to promotion of regeneration and inhibition of liver apoptosis
  • Polypeptide CAK18N and application thereof to promotion of regeneration and inhibition of liver apoptosis

Examples

Experimental program
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Effect test

Embodiment 2

[0039] Example 2 Detection of the activity of polypeptide CAK18N in promoting the proliferation of liver cells in vitro

[0040] In this example, the human liver cell line LO2 was purchased from ATCC.

Embodiment 1

[0041] In this example, the method for detecting the activity of the polypeptide CAK18N described in Example 1 in promoting the proliferation of liver cells in vitro is as follows:

[0042] After LO2 cells were revived according to conventional methods, they were placed at 37°C and 5% CO 2 In the incubator, culture with DMEM complete medium containing 10% FBS and 1% double antibody. When the cells have grown to about 85% of the bottom of the bottle, digest with trypsin-EDTA, add DMEM complete medium and blow gently to make a cell suspension, adjust the cell concentration, inoculate into a 96-well plate, 100 μL per well, Then place at 37°C, 5% CO 2 cultured in an incubator until use.

[0043]Inoculate LO2 in the logarithmic phase at a density of 5000 / well in a 96-well plate and culture for 24 hours. Divided into normal control group, model control group, CAK18N low dose (0.1 μg / mL), medium dose (1 μg / mL), high dose (10 μg / mL) treatment group and positive control group, with ...

Embodiment 3

[0046] Example 3 In vitro activity detection of polypeptide CAK18N inhibiting liver cell apoptosis

[0047] In this example, the method for detecting the activity of the polypeptide CAK18N described in Example 1 in inhibiting liver cell apoptosis in vitro is as follows:

[0048] Human hepatocytes HL-7702 were spread in 96-well plates, and the cell concentration was 1×10 5 cells / ml, 100 μl per well, that is, 1×10 cells per well 4 indivual.

[0049] Each well was treated with 20ng / mL actinomycin D2 (Act D2) for 30min and 80ng / ml tumor necrosis factor α (TNF-α) for 48h to establish the apoptosis model.

[0050] The corresponding polypeptide CAK18N was added for treatment, the dosage volume was 10 μl, and the final concentrations were 0.1, 1, and 10 μg / ml respectively, and the same volume of PBS was used as a negative control, and 0.2 μg / ml epidermal growth factor (EGF) was used as a positive control.

[0051] After drug treatment for 24 hours, add 100 μl Caspase- Reagent (ca...

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PUM

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Abstract

The invention relates to polypeptide CAK18N and application thereof to promotion of regeneration and inhibition of liver apoptosis. The invention discloses polypeptide and application thereof of promotion of hepatic cell proliferation and / or inhibition of liver apoptosis. In the polypeptide capable of promoting hepatic cell proliferation and / or inhibiting liver apoptosis, the amino acid sequence has sequence consistency of more than 16 / 18 through comparison with the amino acid sequence as shown in SEQ ID NO:1, the length of the polypeptide is 18 pieces of amino acid, and the polypeptide has the advantages of small molecular weight, easiness in synthesis, low immunogenicity and the like, so the polypeptide provided by the invention has wide application prospect in liver disease treatment.

Description

technical field [0001] The invention relates to the technical field of cell proliferation and apoptosis, in particular to a polypeptide CAK18N and its use for promoting liver cell proliferation and / or inhibiting liver cell apoptosis. Background technique [0002] The liver is the largest internal organ in the human body (accounting for about 2.5% of the total body mass), and it is also the central metabolic organ. Parenchymal cells are mainly responsible for liver function, accounting for 80% of the total liver cells. Hepatocytes play a series of roles, including producing plasma, synthesizing carrier proteins, detoxifying digests, conjugating and secreting hormones, regulating the synthesis and metabolism of blood lipids and amino acids, etc. [0003] Apoptosis is an active suicide process of cells under specific circumstances. Apoptosis is two different patterns of apoptosis and death observed by Kerr et al. in 1972 when studying liver ischemia. Apoptosis is characteriz...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P1/16
CPCC07K7/08A61P1/16A61K38/00Y02A50/30
Inventor 许元生汤宇晴吴才梅苏博雅
Owner LINK HEALTH GRP
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