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Traditional Chinese medicine preparation detection method

A detection method and a technology of traditional Chinese medicine preparations, which are applied in the preparation of test samples and the measurement of color/spectral characteristics, etc., and can solve the problems of no content determination items, etc.

Active Publication Date: 2020-02-07
JIUZHITANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the current quality standard, only the TLC identification method for Radix Polygoni Multiflori and Mulberry is included, and there is no content determination item

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Yilingjing batch number: 20090821

[0052] 1 TLC identification

[0053] 1.1 Identification of Radix Polygoni Multiflori Radix by TLC method: take 40ml of Yilingjing, put it in a separatory funnel, add dichloromethane to shake and extract 3 times, add 40ml of dichloromethane once at a time, separate the dichloromethane liquid, put it on a water bath and evaporate to dryness , the residue was dissolved by adding 1ml of methanol, and used as the test solution; another 40ml of the negative sample of Radix Polygoni Multiflori Lack was taken, and the negative control solution was prepared in the same way; then 0.2g of Polygonum multiflorum control drug was taken, 20ml of dichloromethane was added, and ultrasound was performed for 30min. Filtrate, evaporate the filtrate to dryness, add 1ml of methanol to the residue to dissolve, and use it as a reference medicinal material solution; according to the TLC test of the Chinese Pharmacopoeia 2015 Edition Four General Rules 0502, a...

Embodiment 2

[0063] Yilingjing lot number: 20090901

[0064] Determination of stilbene glycosides in Radix Polygoni Multiflori

[0065] Chromatographic conditions: chromatographic column is Phenomenex C18 column (250mm×4.6mm, 5μm); detection wavelength is 310nm; mobile phase A is acetonitrile, mobile phase B is water, analysis time is 30 minutes, isocratic elution, mobile phase A: mobile phase The volume ratio of B is 20:80; the flow rate is 0.8mL min -1 ;The column temperature is 40°C; the injection volume is 10μl; the number of theoretical plates is not less than 2000;

[0066] Preparation of reference substance solution: Accurately weigh 13 mg of stilbene glycoside (2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside) reference substance, put it in a 50ml volumetric flask, Add dilute ethanol to dissolve and set the volume to the mark, shake well to obtain the reference substance solution mother liquor. Precisely measure 1mL of the mother solution of the reference substance solution and p...

Embodiment 3

[0070] Yilingjing batch number: 20090903

[0071] Determination of stilbene glycosides in Radix Polygoni Multiflori

[0072] Chromatographic conditions: chromatographic column is Phenomenex C18 column (250mm×4.6mm, 5μm); detection wavelength is 330nm; mobile phase A is acetonitrile, mobile phase B is water, analysis time is 30 minutes, isocratic elution, mobile phase A: mobile phase The volume ratio of B is 20:80; the flow rate is 1.2mL min -1 ;The column temperature is 25°C; the injection volume is 10μl; the number of theoretical plates is not less than 2000;

[0073] Preparation of reference substance solution: Accurately weigh 13 mg of stilbene glycoside (2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside) reference substance, put it in a 50ml volumetric flask, Add dilute ethanol to dissolve and set the volume to the mark, shake well to obtain the reference substance solution mother liquor. Precisely measure 1mL of the mother solution of the reference substance solution and...

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Abstract

The invention relates to a traditional Chinese medicine preparation detection method. The preparation is prepared from seven medicinal materials including radix polygoni multiflori preparata, fructusrosae laevigatae pulp, mulberries, glossy privet fruits, herba siegesbeckiae, radix cyathulae and semen cuscutae, and has the effects of tonifying liver and kidney and benefiting essence marrow. The detection method comprises the following steps: identifying the radix polygoni multiflori preparata, the mulberries, the radix cyathulae and the glossy privet fruits; and determining the content of stibene glucoside in the radix polygoni multiflori preparata in the preparation.

Description

technical field [0001] The invention relates to a detection method of a traditional Chinese medicine preparation, which belongs to the technical field of traditional Chinese medicine. Background technique [0002] Yilingjing is recorded in the "Ministry of Health Drug Standards of the People's Republic of China Chinese Medicine Formula Sixth Volume", and it is the exclusive product of Jiuzhitang Co., Ltd. The preparation is composed of seven kinds of medicinal flavors, including Polygonum multiflorum multiflorum, Rosa japonica meat, mulberry, Ligustrum lucidum (steamed with wine), Utterus chinensis (steamed with honey wine), Achyranthes bidentata (steamed with wine) and Semen Cuscuta (steamed with wine). The effect of nourishing the liver and kidney and benefiting the essence is clinically used for dizziness, dizziness, tinnitus, palpitation, fatigue, dry throat, insomnia and other diseases caused by liver and kidney deficiency. [0003] In the current quality standard, onl...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28
CPCG01N21/33G01N1/28Y02A50/30
Inventor 徐向平夏新华黄胜朱丽谢安欧金秀叶惠煊
Owner JIUZHITANG
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