Production method for removing lipoprotein from horse serum

Abstract

The present invention provides a production method for removing lipoprotein from horse serum. The production method for removing lipoprotein from horse serum comprises the following steps: rough filtering of horse serum through sterile gauze; preliminarily filtering a filtrate A through a filtering column; passing a filtrate B through a modified cellulose acetate filter membrane with a pore size of 0.05-1 [mu]m; conducting ultra-filtering by passing an obtained filtrate C through a hollow fiber ultrafiltration column with a molecular weight cut-off lower than 100 kD; placing the filtrate C obtained by the ultrafiltration in C18 resin for chromatography; placing a chromatographic effluent in a reaction tank and adding ethanol for precipitation; adding a Celite 503 adsorbent to a supernatantA; and adding a polysaccharide microsphere adsorbent to a supernatant B to prepare a lipoprotein-removed horse serum finished product. The method is simple and fast, stable in processes and also suitable for mass production, and can effectively remove lipoproteins and fibrin-containing proteins in the horse serum through layer-by-layer filtration and separation.

Description

technical field

[0001] The invention relates to a method for separating lipoproteins, in particular to a production method for removing lipoproteins from horse serum. Background technique

[0002] Human and animal blood is rich in proteins and enzymes that can heal and save lives. Some proteins are in red blood cells and some are in serum. Since the middle of the last century, the purification and separation of proteins has become an important direction of research. So far, the proteins that can be separated include: albumin, lipoprotein, fibrin, immunoglobulin G, etc.

[0003] Horse serum is a large amount of natural medium used in the cultivation of cells and microorganisms. It is rich in nutrients necessary for the growth of cells and microorganisms. It is often used in the in vitro cultivation of animal cells and the cultivation of bacteria, mycoplasma, etc., and has extremely important functions. However, the horse serum currently available on the market is all deriv...

Examples

Embodiment Construction

[0025] In order to enable those skilled in the art to better understand the technical solutions of the present application, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the implementation of the present application. For example, based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.

[0026] Select an adult horse that has not eaten, and check the horse's body file: including the horse's weight, age, health status, whether there has been an epidemic in the past year, vaccinations in the past year, etc., and register the above content into the blood file of the horse to be blooded middle.

[0027] The selected horses to be blooded were shaved, cleaned and disinfected, and the whole body was d...

technical field

[0001] The invention relates to a method for separating lipoproteins, in particular to a production method for removing lipoproteins from horse serum. Background technique

[0002] Human and animal blood is rich in proteins and enzymes that can heal and save lives. Some proteins are in red blood cells and some are in serum. Since the middle of the last century, the purification and separation of proteins has become an important direction of research. So far, the proteins that can be separated include: albumin, lipoprotein, fibrin, immunoglobulin G, etc.

[0003] Horse serum is a large amount of natural medium used in the cultivation of cells and microorganisms. It is rich in nutrients necessary for the growth of cells and microorganisms. It is often used in the in vitro cultivation of animal cells and the cultivation of bacteria, mycoplasma, etc., and has extremely important functions. However, the horse serum currently available on the market is all deriv...

Examples

Embodiment Construction

[0025] In order to enable those skilled in the art to better understand the technical solutions of the present application, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the implementation of the present application. For example, based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts shall fall within the scope of protection of this application.

[0026] Select an adult horse that has not eaten, and check the horse's body file: including the horse's weight, age, health status, whether there has been an epidemic in the past year, vaccinations in the past year, etc., and register the above content into the blood file of the horse to be blooded middle.

[0027] The selected horses to be blooded were shaved, cleaned and disinfected, and the whole body was d...

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