High-yield bacterial strain of Hubei ophiopogonpolysaccharide and application of high-yield bacterial strain

A high-yielding technology of Ophiopogon japonicus polysaccharides, applied in the field of microorganisms, can solve the problems of long root tuber production cycle, great influence of climatic conditions, and high cost of polysaccharide extraction, and achieve the effects of avoiding unfavorable factors, avoiding pollution, and having great application value

Active Publication Date: 2020-02-28
HUBEI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual planting production of Ophiopogon japonicus in Hubei, there are factors such as "continuous cropping obstacles lead to relatively small planting area", "greatly affected by climatic conditions", "long production cycle of root tubers", "high cost of polysaccharide extraction", etc. As a result, the market release of Hubei Ophiopogon japonicus polysaccharides is restricted
At present, there is no report of an endophytic bacterium capable of producing Hubei Ophiopogon japonicus polysaccharides through microbial fermentation and its application

Method used

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  • High-yield bacterial strain of Hubei ophiopogonpolysaccharide and application of high-yield bacterial strain
  • High-yield bacterial strain of Hubei ophiopogonpolysaccharide and application of high-yield bacterial strain
  • High-yield bacterial strain of Hubei ophiopogonpolysaccharide and application of high-yield bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The isolation step of the high-yield bacterial strain of the Ophiopogon japonicus polysaccharide of embodiment 1

[0049] (1) Sampling: Multiple samples were taken from Hubei Ophiopogon japonicus GAP base, the origin of Hubei Ophiopogon japonicus in Oumiao Town, Xiangyang City, Hubei Province, as plant raw materials for the isolation of high-yielding bacterial strains of Ophiopogon japonicus in Hubei.

[0050] (2) Medium preparation:

[0051] The formula of PDA medium: 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of distilled water

[0052] (3) Isolation of strains: Use a large amount of tap water to fully wash the soil on the surface of fresh Hubei Ophiopogon japonicus plant roots, then put it into a clean beaker, add deionized water, and place it in an ultrasonic cleaner for repeated cleaning until the water after cleaning becomes extremely Clear; use sterile filter paper to dry the moisture on the surface of Ophiopogon japonicus root tube...

Embodiment 2

[0053] The preparation step of embodiment 2 Hubei Ophiopogon japonicus polysaccharide

[0054] Take the high-yielding bacterial strain of Ophiopogon japonicus polysaccharides obtained in Example 1, under aseptic conditions, pick a small number of colonies with an inoculation needle, insert them into a sterilized PDA medium test tube, and activate and cultivate them at 37°C for 16 hours.

[0055] The activated cultured strains were taken, transferred into sterilized liquid potato seed medium under aseptic conditions, and vibrated at 180 rpm at 37° C. for 16 hours to obtain seeds.

[0056] Put the prepared liquid fermentation medium into 250mL Erlenmeyer flasks, about 100mL per bottle, sterilize and cool for later use; under sterile conditions, insert the seeds according to 10% of the inoculum amount, and shake at 180rpm at 37°C 2 days.

[0057] After the fermentation is completed, the fermented solid-liquid mixture is quickly frozen into a solid state in an ultra-low temperatu...

Embodiment 3

[0062] Example 3 Identification of bacterial strains and detection of polysaccharides produced by the bacteria in Ophiopogon japonicus

[0063] (1) Identification of the morphological characteristics of the culture medium

[0064] The high-yielding bacterial strain of Ophiopogon japonicus polysaccharides obtained in Example 1 was streaked on a PDA medium plate, cultured at 28° C. for 24 hours, and characteristics such as colony shape, size, and color were observed. Observation results such as figure 1 As shown, the colony of the high-yield bacterial strain of Ophiopogon japonicus polysaccharides in Hubei was white, round and smooth.

[0065] (2) 16S rRNA gene sequencing of a high-yielding bacterial strain of Ophiopogon japonicus polysaccharides in Hubei

[0066] The 16Sr RNA gene sequence of the high-yielding bacterial strain of Ophiopogon japonicus in Hubei is shown in the attachment, and the sequencing results were compared on the NCBI website (http: / / blast.ncbi.nlm.nih.go...

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Abstract

The invention discloses a high-yield bacterial strain of Hubei ophiopogonpolysaccharide and application of the high-yield bacterial strain. The taxonomic name of the high-yield bacterial strain of the Hubei ophiopogonpolysaccharide is Microbacterium testaceum EBPDAK006, and the preservation number is CCTCC NO: M2018785. According to the high-yield bacterial strain of the Hubei ophiopogonpolysaccharide, the Hubei ophiopogonpolysaccharide can be obtained through liquid fermentation, the problem of difficulty in production of the Hubei ophiopogonpolysaccharide can be effectively solved, and theHubei ophiopogonpolysaccharide can be rapidly and industrially produced in a microbial fermentation mode on a large scale.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a high-yield bacterial strain of Ophiopogon japonicus polysaccharides from Hubei and its application. Background technique [0002] Hubei Ophiopogon japonicus polysaccharide is one of the main active ingredients of Hubei Ophiopogon japonicus Lir iopespicata var. It has pharmacological effects on thrombosis, hypoxia resistance, anti-aging, hypoglycemia, etc. It has a positive impact on improving the body's immune system and enhancing gastrointestinal motility. The latest research shows that it also has anti-tumor effects. [0003] The traditional Hubei Ophiopogon japonicus polysaccharide production is mainly obtained by directly separating the tuber root extract of Ophiopogon japonicus. However, in the actual planting production of Ophiopogon japonicus in Hubei, there are factors such as "continuous cropping obstacles lead to relatively small planting area", "greatly affected by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P19/04C12R1/01
CPCC12N1/02C12N1/20C12P19/04C12N1/205C12R2001/01
Inventor 余海忠郑炜炜张得祥田文清于博王海燕黄升谋阎华孙永林李云捷
Owner HUBEI UNIV OF ARTS & SCI
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