Bactericidal composite containing prothioconazole and nisin
A technology of nisin and prothioconazole, which is applied in the direction of fungicides, biocides, biocides, etc., can solve problems such as unsatisfactory control effects, increased resistance of pathogenic bacteria, and difficult disease control, etc., to achieve synergistic growth Obvious effect, long-lasting effect, not easy to resist
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[0022] Example 1 Indoor virulence determination of prothioconazole and nisin paired with wheat powdery mildew
[0023] Indoor bacteriostatic effect determination method: The growth rate method is used to determine the inhibitory effect of the bactericidal composition on wheat powdery mildew. From the edge of the wheat rot disease colony cultured on the PDA medium for 4 days, take the hyphae blocks with a diameter of 5mm and inoculate them on the PDA medium plates containing different concentrations of fungicides, and cultivate them at a constant temperature at 28℃. In contrast, the colony diameter of each treatment was measured after 4 days, and the percentage of inhibition of the growth of the mycelium was calculated. Through regression analysis of the logarithm of each treatment dose and the probability value of the mycelial growth inhibition rate, calculate the inhibitory concentration of the treated fungicide against wheat powdery mildew (EC s0 ), and then calculate the co-to...
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[0033] Example 2 Indoor virulence determination of prothioconazole and nisin paired with wheat sharp eyespot
[0034] Indoor antibacterial effect determination method: the growth rate method is used to determine the inhibitory effect of the bactericidal composition on wheat sharp eyespot. From the edge of the wheat sheath blight colony cultured on the PDA medium for 4 days, take the hyphae blocks with a diameter of 5mm and inoculate them on the PDA medium plates containing different concentrations of fungicides, and cultivate them at a constant temperature at 28℃. In contrast, the colony diameter of each treatment was measured after 4 days, and the percentage of inhibition of the mycelial growth of the agent was calculated. Through regression analysis of the logarithm of each treatment dose and the probability value of the mycelial growth inhibition rate, the inhibitory concentration of the treated fungicide against wheat sheath blight (EC 50 ), and then calculate the co-toxicity...
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[0043] Example 3 Indoor virulence determination of prothioconazole and nisin paired with wheat fusarium wilt
[0044] Indoor bacteriostatic effect determination method: The growth rate method is used to determine the inhibitory effect of the bactericidal composition on wheat fusarium wilt. From the edge of the wheat root rot disease colony cultured on the PDA medium for 4 days, take the hyphae blocks with a diameter of 5mm and inoculate them on the PDA medium plates containing different concentrations of fungicides, and cultivate them at a constant temperature at 28℃. In contrast, the colony diameter of each treatment was measured after 4 days, and the percentage of inhibition of the growth of the mycelium was calculated. The logarithm of each treatment dose and the probability value of the mycelial growth inhibition rate were used to perform regression analysis to calculate the inhibitory concentration of the treated fungicide against wheat wilt (EC 50 ), and then calculate the ...
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