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Application of rice histone deacetylase gene hda710 in delaying leaf senescence
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A HDA710, protein technology, applied in application, hydrolase, genetic engineering, etc., can solve problems such as unclear and limited plant photosynthesis cycle, mechanism research, etc.
Active Publication Date: 2021-07-27
CHINA AGRI UNIV
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[0003] Leaf senescence is an important stage of plant development. In agricultural production, early leaf senescence limits the plant photosynthesis cycle, thereby affecting crop yield
As the world's main food crop, rice mainly includes two subspecies. Previous studies have shown that there are differences in leaf senescence in indica and japonica rice. The leaf senescence of indica rice is earlier than that of japonica rice, but the specific mechanism is still unclear.
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Embodiment 1
[0065] Example 1, Preparation and Identification of HDA710 Gene Transgenic Plants
[0066] First, the construction of recombinant plasmid
[0067] 1. Extract the total RNA in Japan in rice, and reverse transcription with total RNA as a template to obtain cDNA.
[0068] 2. The cDNA obtained in step 1 is a template, and the PCR amplification is recovered using the primer composed of SENSE-VECTOR-F and SENSE-VECTOR-R to recover the PCR amplification product.
[0071] 3. Take the PCR amplification product obtained by step 2, perform bisase digestion and recovery with restriction endonuclease XBAI and KPNI.
[0072] 4, the carrier PCAMBIA1300 is taken, and the carrier skeleton is recovered with restriction endonuclease XBAI and KPNI.
[0073] 5. Connect the enzyme digestion obtained by step 3 and the carrier skeleton obtained by step 4, and the recombinant pla...
Embodiment 2
[0138] First, use the RPAN website to dig the key genes of indea rice and japonica rice and related to phenotypes
[0139] EtOAc EtOAc EtOAc EtOAc EtOAc http: / / cgm.sjtu.edu.cn / 3kricedb / The website is searching, finding five sub-seeds in rice [JAP (japonica), Indica), AUS (similar varieties of Japonica rice), ARO (Japonica rice similar variety), ADM (indea rice and japonica rice varieties)] frequency. Two fragments of the HDA710 gene are manually viewed through the UCSC browser of different varieties. See Image 6 . From Rice Snp-seekDatabase ( Https: / / snp-seek.irri.org / _download.zul The website downloads the phenotypic data of 2266 varieties on the website, and the variety of the HDA710 gene fragment is related to the phenotypicity, see Figure 7 .
[0140] Second, the comparison of the characteristics of rice in the rice, rice 9311 and rice special blue
[0141] Rice Japan is sunny in japonica rice. Rice 9311 belongs to Japonica rice. Rice specialty is odda rice. Compared to Japon...
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Abstract
The invention discloses the application of rice histone deacetylase gene HDA710 in delaying leaf senescence. The present invention provides a new use of rice HDA710 protein, that is, the application in regulating the leaf senescence process of plants. The transgenic plants overexpressing the HDA710 gene had increased chlorophyll content and decreased electrical conductivity, which was in line with the characteristics of late leaf senescence. Transgenic plants with low expression of the HDA710 gene are in line with the characteristics of premature leaf senescence. The present invention provides a new direction for promoting the application of epigenetics and genetic variation in crop breeding.
Description
Technical field [0001] The present invention relates to the application of rice group protein deacetylated enzyme gene HDA710 in delayed blade aging. Background technique [0002] Optimistic genetic changes can be re-programmed in various biological processes. The deacetylation of histones plays an important regulation effect in various adverse stresses of plant development and response. In rice, 18 histone deacetylase genes have been reported. The HDA710 / OSHDAC2 belongs to the I-type histone deacetylase. [0003] Blade aging is an important stage of plant development. In agricultural production, early blade aging limits the plant photosynthetic work cycle, which affects the output of crops. Rice as the world's major food crops, mainly including two subspecies, prior to research showed that there is a difference between the blade aging in otica rice and japonica rice, and the blade aging of odda rice has been long in japonica rice, but the specific mechanism research is not cle...
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