Method for improving quality of citrus fruits by using inoculation of arbuscular mycorrhizal fungi

An arbuscular mycorrhizal fungus and citrus technology, which is applied in the field of gardening, can solve the problems of increased production cost of inorganic fertilizers, decreased citrus fruit quality benefits, and decreased fruit quality.

Inactive Publication Date: 2020-03-20
YANGTZE UNIVERSITY
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  • Application Information

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Problems solved by technology

Citrus is an important industrial structure for farmers to get rich, but with the increase of area and output, the quality and benefit of citrus fruit decline
In

Method used

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  • Method for improving quality of citrus fruits by using inoculation of arbuscular mycorrhizal fungi
  • Method for improving quality of citrus fruits by using inoculation of arbuscular mycorrhizal fungi
  • Method for improving quality of citrus fruits by using inoculation of arbuscular mycorrhizal fungi

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] 1. The mixed-AMF strain is provided by the "Chinese Arbuscular Mycorrhizal Fungi Germplasm Library (BGC)", and then expanded by white clover (Trifolium repens L.) for 16 weeks and stored at 4℃ for later use; mixed-AMF is provided by D .versiformis, F.mosseae and R.intraradices according to the 1:1:1 mixed strain (mixed-AMF);

[0021] 2. Inoculation treatment: inoculate 5 mixed-AMF; the AMF inoculum is composed of spores, mycorrhizal hyphae and root segments, and 600g of mycorrhiza is inoculated into the citrus root system;

[0022] 3. Maintenance and management: The fertilization ditch includes the north-south fertilization ditch and the east-west fertilization ditch for citrus trees; for the first time, two fertilizations are selected from the north-south fertilization ditch or the east-west fertilization ditch; the second fertilization is the first fertilization In the next 11 to 13 months, fertilize in the other two fertilizing furrows, specifically:

[0...

Example Embodiment

[0029] Experimental example 1

[0030] 1. The following indicators of Example 1 and Comparative Example 1-2 were measured, and the results are shown in Table 1.

[0031] (1) Mycorrhizal infection rate refers to the method described by Phillips and Hayman (1970) for staining; the calculation formula of mycorrhizal infection rate is as follows: Mycorrhizal infection rate (%) = length of AMF colonization root segment / total observation root segment Length × 100%.

[0032] (2) Soil hypha density: Determine the soil hypha density according to the method of Bethlenfalvay and Ames (1987).

[0033] (3) Root vitality: The root vitality was analyzed according to the triphenyltetrazolium chloride method described by Lindstrom and Nystrom (1987).

[0034] SAS 8.1 one-way analysis of variance (ANOVA) was used, and Duncan's multi-range test was used to compare the significance of the differences between treatments (p=0.05).

[0035] Table 1 Effect of mixed-AMF inoculation on the root system and root v...

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Abstract

The invention discloses a method for improving quality of citrus fruits by using inoculation of arbuscular mycorrhizal fungi. The method comprises the step of inoculating a mixed strain of arbuscularmycorrhizal fungi in citrus root systems, wherein the mixed strain of arbuscular mycorrhizal fungi comprises D. versiformis, F. mosseae and R. intraradices in a spore ratio being 1:1:1. By inoculatingthe mixed strain of arbuscular mycorrhizal fungi (D. versiformis, F. mosseae and R. intraradices in the spore ratio being 1:1:1) in the citrus root systems, the mixed strain of arbuscular mycorrhizalfungi as efficient and safe bio-fertilizer is found to have significant improvement effects on growth and development of citrus and improvement of fruit quality, and besides, as a renewable and sustainable resource, the ecological and economic benefits and environmental sustainability effects of the mixed strain of arbuscular mycorrhizal fungi are superior to those of other bio-fertilizers.

Description

technical field [0001] The invention relates to the technical field of gardens, in particular to a method for improving the quality of citrus fruits by inoculating arbuscular mycorrhizal fungi. Background technique [0002] Citrus is an evergreen fruit tree that is widely grown all over the world. Citrus (Citrus L.) is an important economic crop and fruit tree resource in my country. As of 2017, my country's citrus planting area and output rank first in the world. The main production areas of citrus are concentrated in the south of the Yangtze River. Chongqing, Sichuan, Hunan, Hubei, Jiangxi, Guangxi, Guangdong and other provinces and municipalities. Citrus is an important industrial structure for farmers to get rich, but with the increase of area and output, the quality and benefit of citrus fruit decline. In field production, the input of inorganic fertilizers greatly increases production costs, and at the same time easily causes a decline in fruit quality. How to develo...

Claims

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Application Information

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IPC IPC(8): A01G17/00A01G7/06A01C21/00
CPCA01C21/005A01G7/06A01G17/005
Inventor 吴强盛成晓分曹金丽张菲邹英宁刘春艳张德健
Owner YANGTZE UNIVERSITY
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