A single mammalian cell nano-encapsulation method and the resulting encapsulated cell

Abstract

The invention discloses a nano-encapsulation method of a single mammalian cell and the obtained encapsulation cell, belonging to the field of biomedicine. In this method, mammalian cells are first made into a single-cell suspension, and then added to a solution of a positively charged encapsulation matrix for reaction; after the reaction is completed, the cells are washed and resuspended in a solution of a negatively charged encapsulation matrix, and Add transglutaminase for reaction to obtain encapsulated cells with 2 layers of encapsulation; repeat the above steps n times to obtain encapsulated cells with 2n layers of encapsulation, and make an encapsulated cell suspension; Add transglutaminase to the suspension, conduct cross-linking reaction at 20-37°C for 20-120min with slight shaking, collect cells by centrifugation and wash to obtain mammalian single cells with 2n layers of surface encapsulation. The encapsulated cells prepared by the invention have high survival rate, compact encapsulation layer and long maintenance time, and all encapsulation materials have no cytotoxicity.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a single mammalian cell nano-encapsulation method and the obtained encapsulated cells. Background technique [0002] The purpose of cell therapy is to replace or repair damaged tissues and organs. At present, cell suspension can be directly injected into the tissue or cavity through a syringe or catheter to achieve cell therapy. However, due to the high pressure and shear stress generated during injection and immune rejection in vivo, the survival rate of transplanted cells and the duration of circulation in vivo are significantly reduced. [0003] Cell encapsulation technology provides a new way to solve the above problems in the process of cell therapy. The role of the encapsulation matrix material is similar to that of the extracellular matrix. On the one hand, the encapsulation layer needs to have good selective permeability, which can ensure the free exchang...

AI Technical Summary

Problems solved by technology

However, in addition to the cytotoxicity of the polyelectrolytes used (PDADMAC, PSS, TA, PVPON, etc.), the existing single-cell encapsulation technologies that only use LbL technology to construct the encapsulation layer outside the cell have a loose structure and a short maintenance period. Relatively shortcoming, making it unable to provide long-term reliable immune protection for encapsulated cells

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