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Treatment method for promoting germination of curculigo orchioides seeds

A treatment method and seed technology, applied in the fields of medicinal plant biology and seed biology, can solve the problems of difficult germination and long germination time of curculigo seeds, improve water absorption efficiency and germination, increase seed germination rate, and facilitate operation Effect

Active Publication Date: 2020-03-27
贵州培力农本方中药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to overcome the problems of difficult germination and long germination time of curculigo seeds in the prior art, thereby providing a method that can greatly improve the germination rate (up to 83%) of curculigo seeds that are different from other methods new method

Method used

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  • Treatment method for promoting germination of curculigo orchioides seeds
  • Treatment method for promoting germination of curculigo orchioides seeds
  • Treatment method for promoting germination of curculigo orchioides seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the aseptic culture of curculigo seed:

[0023] Main instruments: sterilization pot, ultra-clean workbench.

[0024] Curculigo curculigo seed aseptic culture of embodiment 1 carries out according to the following steps:

[0025] (1) Seed picking: pick off the mature seed pods and dry them in the shade with the peel; partially burst seeds fall into the soil and collect them with tweezers for later use.

[0026] (2) Seed screening: rub the dried fruit pods to remove impurities, put the seeds together with the seeds scattered in the soil into water for screening, remove the floating seeds on the upper layer, collect the strong and full seeds in the lower layer, and wash them. After drying in the shade, store in a 4°C refrigerator for later use.

[0027] (3) Preparation of explant cleaning solution: put 3 drops of detergent and 1 drop of 84 disinfectant into 1L of distilled water, stir evenly, and set aside the seed cleaning solution.

[0028] (4) Cleaning ...

Embodiment 2

[0040] Embodiment 2: Complete seed compares with removing outer testa seed germination:

[0041] Example 2 is to investigate the effect of removing the testa on the germination of Curculigo curculigo seeds by comparing complete Curculigo curculigo seeds and Curculigo curculigo seeds from which the outer testa has been removed.

[0042] Curculigo seed outer testa removal method:

[0043] (1) Take out the curculigo seeds screened in Example 1, put about 50 seeds into the mortar each time, place a piece of gauze of 15 × 15cm to cover on the mortar, place it on the gauze with a mortar stick Grinding, the strength is controlled so that the seed coat can be crushed without destroying the inside of the seed.

[0044] (2) Pick out the peeled seeds with small pointed tweezers and place them in 5mL centrifuge tubes, 60 seeds per tube.

[0045] (3) The unpeeled seeds continue to grind until all the seeds in the mortar are completely peeled, and the seed coats are discarded after comple...

Embodiment 3

[0050] Embodiment 3: gibberellin (GA 3 ) on the germination of Curculigo curculigo seeds:

[0051] Example 3 is to compare the effects of different concentrations of gibberellin on the germination of Curculigo curculifolia seeds by soaking them with different concentrations of gibberellin, and screen out an optimal treatment concentration.

[0052]The curculigo seeds with the outer testa removed were put into GA with concentrations of 10mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L, and 300mg / L respectively. 3 The solution was soaked for 24 hours, and the seeds were soaked in distilled water as a control, and the seeds were aseptically germinated. Carry out operation according to the step of embodiment example 1.

[0053] Table 3 Effects of soaking treatments with different concentrations of gibberellin on the germination of Curculigo curculigo seeds

[0054]

[0055]

[0056] The experimental results showed that when the gibberellin concentration was 50mg / L, the germinatio...

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Abstract

The invention discloses a treatment method for promoting germination of curculigo orchioides seeds. The treatment method comprises the steps of collecting maturing curculigo orchioides seeds, removingseed coats, performing soaking in gibberellin and performing germ-free germination, wherein the concentration of the gibberellin for soaking is 100mg / L, the soaking time is 24h, and the germ-free germination is performed on a superclean bench; performing sterilization treatment on the seeds treated with the gibberellin, inoculating the sterilized seeds with culture mediums, and performing cultureunder the appropriate culture condition, wherein the sterilization treatment is performed through the steps of sterilizing seeds with 75% ethanol for 30s, performing flushing with sterile water twice, then performing sterilization with 0.1% mercury dichloride for 8min, and performing flushing with sterile water for 5 times, the appropriate culture condition refers to the condition of 25 DEG C, 2500Lx and 12 h / d, and the culture mediums consist of MS+6-BA of 1.5 mg / L and 0.8 mg / L, and the pH is 5.8. According to the treatment method disclosed by the invention, a unique method which is not discovered by other people and facilitates promotion of the germination of the curculigo orchioides seeds is combined with a traditional seed germinating method, so that the germination rate of the curculigo orchioides seeds can reach 83% or above.

Description

technical field [0001] The invention relates to the technical field of seed biology and the technical field of medicinal plant biotechnology, and specifically relates to a method for accelerating germination and a method for aseptic seed cultivation by adopting a specific treatment method to break the seed coat barrier of curculigo seeds to improve the germination rate of the seeds. Background technique [0002] Curculigo orchioides is the dried rhizome of Curculigo orchioides Gaertn. Perennial herb, excavated in autumn and winter, remove roots and fibrous roots, wash and dry. Curculigo curculigo is a rare medicinal plant, which has the functions of invigorating kidney yang, strengthening muscles and bones, and dispelling cold and dampness. It is used for impotence, essence cold, weakness of muscles and bones, cold pain in the waist and knees, and cold diarrhea due to yang deficiency. Curculiculatus is rich in wild resources, but due to extensive excavation, the damage is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/001
Inventor 廖宇娟赵锋兰才武贺定翔
Owner 贵州培力农本方中药有限公司
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