Image acquisition method and apparatus, microscope system, computer readable storage medium

Abstract

The present application provides an image acquisition method, device, microscope system, and computer-readable storage medium, which are applied to the microscope system. Collect a first image of the sample to be tested, the first image includes the target body; control the objective lens to collect a second image of the sample to be tested on a plurality of preset planes that are parallel to each other, a plurality of the preset planes intersecting the fitted focal plane; and performing image fusion on a plurality of the second images to obtain a target image, where the target image includes the target subject. The present application acquires a target image by collecting second images on a plurality of preset planes intersecting with the fitting focal plane, and fuses the plurality of second images to obtain a target image. The method uses the target image after fusion of the plurality of second images as a mirror The detection image can improve the quality of the microscopic image, improve the efficiency of microscopic inspection, and reduce the probability of missed detection of the target subject.

Description

technical field [0001] The present application relates to the technical field of sample identification and image processing, and in particular, to an image acquisition method and device, a microscope system, and a computer-readable storage medium. Background technique [0002] In the process of disease diagnosis and scientific research, it is often necessary to sample and film the specimen to be examined, and use the method of microscopic examination to observe, analyze and judge the image of the sample under a high-power or low-power microscope, and provide sample identification. Microscopic results. Human excreta, secretions, exfoliated cells or human tissues, animal tissues, and even plant cells can be used as microscopic examination objects. [0003] However, due to the sample container processing accuracy is not flat enough, the counting plate, blood smears are placed tilted, and there is shaking during the mechanical transmission process, etc., the target subjects in ...

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Problems solved by technology

[0003] However, due to the uneven processing precision of the sample container, the tilting of the counting plate and blood smear, and the shaking during the mechanical transmission process, the target subjects in all viewing areas may not be on the same preset plane. By adjusting the stage or objective lens It is very difficult to compensate for the slight error of the sample container by the height of the sample, so the quality of the collected microscopic image is poor, and the probability of missing the target is high

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