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Adipose tissue fixing solution as well as preparation method and application method thereof

A technology of adipose tissue and fixation method, which is applied in the field of adipose tissue fixative and its preparation, which can solve the problems of poor fixation effect, easy side effects, and high hazard of formaldehyde, and achieve easy flattening, clear adipocyte frame, and preparation The effect of simple method

Inactive Publication Date: 2020-04-10
SUZHOU CANCERCELL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, formaldehyde is more harmful, and it is prone to side effects, resulting in poor fixation effect.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Prepare adipose tissue fixative:

[0039] Take 36 parts of ethanol and 34 parts of salicylic acid and mix evenly, then add 22 parts of formaldehyde and 3 parts of glacial acetic acid and continue mixing to obtain liquid a;

[0040] Take 14 parts of PEG 4000, 2 parts of calcium chloride, 10 parts of ammonium sulfate and 300 parts of water and ultrasonically mix them evenly. The frequency of ultrasonic mixing is 40kHz, the power is 410W, the mixing time is 20min, and the mixing temperature is 60°C Store at ℃ for one night to obtain liquid b;

[0041] The prepared liquid a and liquid b were mixed with a magnetic stirrer, the working speed of the magnetic stirrer was 150 rpm, the temperature was room temperature, and the adipose tissue fixative was obtained after uniform mixing.

[0042] Fixation of adipose tissue:

[0043] After the adipose tissue sample is taken, add adipose tissue fixative, the volume of the added adipose tissue fixative is 10 times the volume of adipo...

Embodiment 2

[0046] Prepare adipose tissue fixative:

[0047] Take 32 parts of ethanol and 36 parts of salicylic acid and mix evenly, then add 18 parts of formaldehyde and 5 parts of glacial acetic acid and continue mixing to obtain liquid a;

[0048] Take 10 parts of PEG10000, 5 parts of calcium chloride, 6 parts of ammonium sulfate and 400 parts of water and ultrasonically mix them evenly. The frequency of ultrasonic mixing is 40kHz, the power is 330W, the mixing time is 30min, the mixing temperature is 40°C, and then placed at 4°C Store overnight under environmental conditions to obtain liquid b;

[0049] The prepared liquid a and liquid b were mixed with a magnetic stirrer, the working speed of the magnetic stirrer was 250 rpm, the temperature was room temperature, and the adipose tissue fixative was obtained after mixing evenly.

[0050] Fixation of adipose tissue:

[0051] After the adipose tissue sample is taken, add adipose tissue fixative, the volume of the added adipose tissue ...

Embodiment 3

[0054] Prepare adipose tissue fixative:

[0055] Take 34 parts of ethanol and 35 parts of salicylic acid and mix evenly, then add 15 parts of formaldehyde and 4 parts of glacial acetic acid and continue mixing to obtain liquid a;

[0056] Take 12 parts of PEG6000, 3.5 parts of calcium chloride, 8 parts of ammonium sulfate and 350 parts of water and ultrasonically mix them evenly. The frequency of ultrasonic mixing is 40kHz, the power is 370W, the mixing time is 25min, the mixing temperature is 50°C, and then placed at 4°C Store overnight under environmental conditions to obtain liquid b;

[0057] The prepared liquid a and liquid b were mixed with a magnetic stirrer, the working speed of the magnetic stirrer was 200 rpm, and the temperature was room temperature, and the adipose tissue fixative was obtained after uniform mixing.

[0058] Fixation of adipose tissue:

[0059] After the adipose tissue sample is taken, add adipose tissue fixative, the volume of the added adipose t...

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Abstract

The invention discloses an adipose tissue fixing solution as well as a preparation method and an application method thereof. The preparation method of the adipose tissue fixing solution comprises thefollowing steps: taking 32-36 parts of ethanol and 34-36 parts of salicylic acid and mixing well, adding 18-22 parts of formaldehyde and 3-5 parts of glacial acetic acid, and continuing to mix well toobtain a solution a; taking 10-14 parts of polyethylene glycol, 2-5 parts of calcium chloride, 6-10 parts of ammonium sulfate and 300-400 parts of water, ultrasonically mixing the materials well, andstoring the mixture in an environment of 4 DEG C for one night to obtain a solution b; and mixing the prepared solution a and the prepared solution b by using a magnetic stirrer, and obtaining the adipose tissue fixing solution after the solution a and the solution b are well mixed. The fixing method comprises the following steps: taking an adipose tissue sample, adding the adipose tissue fixingsolution, fixing the adipose tissue sample at room temperature for 3-24 hours, and carrying out dehydration treatment, transparency treatment and waxing treatment by adopting a full-automatic dehydrator. The adipose tissue fixing solution is simple in preparation method, low in toxicity and easy to operate, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an adipose tissue fixative and its preparation method and application method. Background technique [0002] In the process of pathological diagnosis, specimen preparation is often required. Specimen fixation is crucial to the correctness of pathological diagnosis. For the fixation of adipose tissue, due to its special structure, various factors often occur during the preparation process, resulting in poor specimen preparation, which affects subsequent observation and diagnosis. [0003] Adipose tissue contains a large amount of oil components, and cannot be completely fixed and dehydrated. And when fixing the adipose tissue, it is necessary to consider that the fixative cannot cause the adipose tissue to harden and shrink, otherwise it will affect the film-making effect. [0004] In the prior art, the adipose tissue fixative solution with formaldehyde as the main component is gene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00G01N1/30
CPCA01N1/00G01N1/30
Inventor 王守立赵飞沈刚
Owner SUZHOU CANCERCELL BIOTECH
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