Application of tubeufia rubra in preparation of drugs for reversing tumor multidrug resistance to sensitivity

A multi-drug resistance, susceptibility technology, applied in the field of microbiology, can solve the problems of limitations, unstudied applications, lack of P-glycoprotein specificity, etc.

Active Publication Date: 2020-04-14
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, such drugs usually lack the specificity of P-glycoprotein, which will cause serious side effects, and the first-generation reversal agents are also clinically limited to a large extent (Sato W.et al.1991)
The second-generation reversal agent, valspodar (PSC833), dexverapamil, etc., is represented by dexamethasone. However, the development of the second-generation reversal agent is due to high Limited by a range of side effects due to toxicity and drug interactions (Rowinsky E.K. et al. 1998; Hyafil F. et al. 1993; Keller R.P. et al. 1992)
However, the application of this family of fungi in MDR has not been studied. Therefore, this study attempts to screen effective multidrug resistance reversal agents from Trichocystaceae fungi.

Method used

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  • Application of tubeufia rubra in preparation of drugs for reversing tumor multidrug resistance to sensitivity
  • Application of tubeufia rubra in preparation of drugs for reversing tumor multidrug resistance to sensitivity
  • Application of tubeufia rubra in preparation of drugs for reversing tumor multidrug resistance to sensitivity

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Experimental program
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Effect test

Embodiment 1

[0073] The tubeufia rubra Y.Z.Lu, J.C.Kang & K.D. Hyde PF02-2 strain is obtained from the Chemical Engineering Center of Guizhou University, and the tubeufia rubra Y.Z.Lu, J.C.Kang & K.D. Hyde is named as the tubeufia rubra Y.Z.Lu, J.C.Kang&K.D. Hyde. Depository unit: China Deposited by the Typical Culture Collection Center, the address is: China. Wuhan. Wuhan University, the date of deposit: 2019.11.20, and the deposit registration number is CCTCC M 2019957PF02-2.

[0074] The process of separation of Trichophyllum PF02-2 sample of the present invention is:

[0075] (1) The rotted wood sample collected is placed under a microscope for microscopic examination;

[0076] (2) Sterilize the inoculation needle, pick the white colony on the surface of the rotten wood, and transfer it to 40 μL sterile water to make a spore suspension;

[0077] (3) Transfer the spore suspension to a 5 mL water-agar (abbreviated as WA) plate culture dish, place it at 26 ° C for 10 hours, and pick a si...

Embodiment 2

[0092] The process of separation of Trichophyllum PF02-2 sample of the present invention is:

[0093] (1) The rotted wood sample collected is placed under a microscope for microscopic examination;

[0094] (2) Sterilize the inoculation needle, pick the white colony on the surface of the rotten wood, and transfer it to 80 μL sterile water to make a spore suspension;

[0095] (3) Transfer the spore suspension to a 15mL water-agar (abbreviated as WA) plate culture dish, place it at 30°C for 14 hours, and pick a single conidia that has germinated and grown mycelium and inoculate it into 15mL potato In the glucose agar petri dish (abbreviated as PDA), cultivate at 30°C to obtain pure colonies of PF02-2.

[0096] The reversing of tumor multidrug resistance refers to the activity of reversing the multidrug resistance of cancer cells, resulting in resensitization to anticancer drugs. The reversal activity refers to the inhibitory effect of Trichophyllum rufica PF02-2, its metabolite...

Embodiment 3

[0110] The process of separation of Trichophyllum PF02-2 sample of the present invention is:

[0111] (1) The rotted wood sample collected is placed under a microscope for microscopic examination;

[0112] (2) Sterilize the inoculation needle, pick the white colony on the surface of the rotten wood, and transfer it to 50 μL sterile water to make a spore suspension;

[0113] (3) Transfer the spore suspension to an 8mL water-agar (abbreviated as WA) plate culture dish, place it at 27°C for 11 hours, pick a single conidia that has germinated and grown mycelia and inoculate it into 7mL potato In the glucose agar petri dish (abbreviated as PDA), cultivate at 27°C to obtain pure colonies of PF02-2.

[0114] Trichocolum rufica PF02-2 bacterial classification preservation method of the present invention is:

[0115] (1) Preparation of monospore strain suspension: pick conidia from the isolated PF02-2 pure colony with a sterilized inoculation needle or injection needle next to the fl...

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Abstract

The invention relates to the technical field of microorganisms, in particular to tubeufia rubra for reversing multidrug resistance of tumor cells to sensitivity. The strain is preserved in the China Center for Type Culture Collection, the preservation date is 2019.11.20, and the preservation registration number is CCTCC M 2019957PF02-2. The invention provides a method for obtaining an active metabolite through fermentation of tubeufia rubra PF02-2. The metabolite has the activity of reversing the multidrug resistance of tumor cells to be sensitive, so that the tumor cells are sensitive to thedrug again, the drug effect of the anti-tumor drug can be exerted, and a new way is added for the development of new sources of medical drugs.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of Trichophyllum rufica in the preparation of drugs for reversing tumor multidrug resistance to sensitivity. Background technique [0002] Tumor multidrug resistance (Multidrug Resistance, MDR) refers to the phenomenon that tumor cells not only develop resistance to one antitumor drug, but also cross-resistance to other antitumor drugs with different structures and different mechanisms of action. Although the research on tumor treatment continues to advance, less than 50% of tumors are sensitive to chemotherapy, and more than 50% of tumors rapidly develop resistance to chemotherapy drugs. Once drug resistance occurs, doctors will be helpless. Therefore, finding low-toxicity and high-efficiency reversing agents of tumor drug resistance has become a research hotspot in the field of tumor chemotherapy drugs. [0003] P-glycoprotein (P-gp) is one of the ...

Claims

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Application Information

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IPC IPC(8): A61K36/06A61K45/06A61P35/00A61P35/02C12N1/14C12N1/02C12N1/04C12R1/645
CPCA61K36/06A61K45/06A61K2236/19A61P35/00A61P35/02C12N1/02C12N1/04C12N1/14C12N1/145C12R2001/645
Inventor 康冀川陈丽庄钱一鑫卢永仲范翠王鲁
Owner GUIZHOU UNIV
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