Application of interferon-induced protein 6 and medicine

An interferon induction and drug technology, applied in the biological field, to enhance the anti-apoptosis and cell proliferation ability, and reduce the effect of skin radiation damage

Pending Publication Date: 2020-04-14
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no application of IFI6 in the prevention and treatment of radiation-induced skin injury at home and abroad, and more research and exploration is needed for its mitigating effect on radiation injury.

Method used

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  • Application of interferon-induced protein 6 and medicine
  • Application of interferon-induced protein 6 and medicine
  • Application of interferon-induced protein 6 and medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Construction of IFI6 overexpression lentivirus and expression verification after infection

[0032]In this example, the whole gene synthesis method (Hanbio Shanghai) was used to synthesize the human IFI6 coding region gene (the nucleic acid sequence is shown in Table 1 below), and the IFI6 overexpression lentivirus LV-IFI6 (target lentivirus) was obtained by packaging with a three-plasmid virus packaging system. and LV-NC (control lentivirus) recombinant lentivirus.

[0033] Table 1 The sequences of primers and genes

[0034]

[0035]

[0036] Human skin keratinocytes HaCaT and human skin fibroblasts WS1 in exponential growth phase were mixed with 10.0×10 4 The density of each well was inoculated in a 6-well plate. After the cells adhered to the wall, the MOI value of the virus provided by the instructions was calculated. Add an appropriate amount of IFI6 overexpression lentivirus or control lentivirus (HBLV-NC) to each well, and incubate at 37°C. , 5%...

Embodiment 2

[0037] Example 2 The effect of IFI6 overexpression lentivirus on the proliferation ability of skin cells after irradiation

[0038] Human skin cells stably overexpressing IFI6 in the exponential growth phase and their control cells were inoculated in 96-well plates, and 4 parallel samples were set up in each group. After the cells adhered to the wall, an absorbed dose of 20Gy was given to HaCaT and WS1 cells respectively. and 5Gy X-ray irradiation. 24 hours after exposure, the cells were processed according to the instructions of the Cell-Light Edu Fluorescence Staining Kit provided by Guangzhou Ruibo Company. After the processing was completed, photos were taken with a fluorescence microscope, and the data were counted and processed. The experiment was repeated three times.

[0039] Such as figure 2 As shown (P<0.01), the results showed that IFI6 overexpression lentivirus can significantly promote the proliferation ability of skin cells after irradiation with ionizing radi...

Embodiment 3

[0040] Example 3 The effect of IFI6 overexpression lentivirus on the apoptosis level of skin cells after irradiation

[0041] Human skin HaCaT, WS1 cells and their control cells stably overexpressing IFI6 in the logarithmic growth phase were seeded in 6-well plates, and 20Gy and 5Gy X-rays were irradiated after the cells adhered to the wall, and the cells were collected 48 hours after irradiation, and used BD's Annexin V / 7AAD PE apoptosis detection kit was used to detect cell apoptosis in each group, and three replicate wells were set up in each group.

[0042] Such as image 3 As shown (P<0.01), the results showed that LV-IFI6 did not affect the apoptosis rate of the 0Gy group; after irradiation, compared with the control lentivirus LV-NC group, the apoptosis rate of the IFI6 overexpression group was significantly reduce.

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PUM

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Abstract

The invention provides an application of interferon-induced protein 6 (IFI6) and a medicine. The invention specifically provides an application of IFI6 in a medicine for preventing and/or treating skin cell radiation injury and a medicine containing IFI6. Specifically, the medicine is realized by constructing an IFI6 over-expression strategy, and the over-expression of IFI6 has a prevention and treatment effect on radiation injury of human skin cells and can enhance the anti-apoptosis and cell proliferation capabilities of the radiated cells. The IFI6 over-expression lentivirus or the medicinecontaining the IFI6 over-expression lentivirus can be used for preventing and treating human skin radiation injury, and has important significance and application value in the aspects of coping withnuclear and radiation accidents and nuclear emergencies, reducing the skin radiation injury effect of tumor radiotherapy patients and the like.

Description

technical field [0001] The invention relates to the application of interferon-inducible protein 6 and a medicine for preventing and treating radiation damage of skin cells, and belongs to the field of biotechnology. Background technique [0002] Radiation-induced skin injury is the most common side effect of clinical tumor radiotherapy. In addition, the skin is the largest organ of the human body and is in direct contact with the external environment. In occasional nuclear radiation accidents and long-term occupational exposure, the skin is often the first to be irradiated, resulting in radiation skin damage. In tumor radiotherapy, a considerable number of patients will experience different degrees of radiation skin damage. Severe radiation skin damage can affect the smooth progress of radiotherapy and the quality of life of patients. [0003] Radiation skin damage refers to inflammatory damage caused by direct exposure of ionizing radiation to the skin. According to radi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P17/00
CPCA61K38/1709A61P17/00
Inventor 张舒羽贾慧敏徐小慧洪敏
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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