Application of iron agent in preparation of anti-hepatoma metastasis drugs

A liver cancer metastasis and anti-liver cancer technology, applied in the field of medicine, can solve the problem of decreased iron content in liver cancer tissue, achieve good clinical application prospects, and promote the effect of liver cancer metastasis

Inactive Publication Date: 2020-04-17
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the iron content of liver cancer tissue in patients with liver cancer is significantly reduced. At present, there are no reports on the relationship between iron deficiency in liver cancer and liver cancer metastasis at home and abroad.

Method used

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  • Application of iron agent in preparation of anti-hepatoma metastasis drugs
  • Application of iron agent in preparation of anti-hepatoma metastasis drugs
  • Application of iron agent in preparation of anti-hepatoma metastasis drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Experimental method

[0024] 1. Luciferase labeling of HCC cells

[0025] H22 cells were labeled with luciferase using the luciferase labeling kit, which contains lentivirus-mediated luciferase labeling solution and polybrene, and overexpressed luciferase through the CAG promoter as a reporter gene.

[0026] Infection works as follows:

[0027] (1) The cells are subcultured into a 100mm culture dish overnight, when the cell density does not exceed 60%;

[0028] (2) Mix 1ml of luciferase labeling solution with 3ml of fresh cell culture medium, add 4μl polybrene to make the final concentration 8ng / ml.

[0029] (3) Gently shake the labeled cell culture dish at intervals of 15-30 min for 2 hours, then add an equal volume of fresh culture medium to the culture dish, and change the medium after culturing overnight.

[0030] (4) 48 hours after infection, the labeling effect was detected with a luciferase detection kit.

[0031] 2. Orthotopic tumor-bearing liver in mice ...

Embodiment 2

[0045] 1. Experimental method

[0046] 1. Collection of Liver Cancer Patients

[0047] After the informed consent of the patients and the approval of the Ethics Committee, liver cancer tissues and adjacent tissues from 29 patients diagnosed with liver cancer were collected from the Changhai Hospital Affiliated to the Second Military Medical University and the Dongfang Hepatobiliary Hospital Affiliated to the Second Military Medical University. content. 15 cases of surgical resection specimens of patients with liver cancer metastasis within 5 years after liver cancer surgery and 15 cases of surgical resection specimens of patients without metastasis within 5 years were obtained from the biological specimen platform of Liver Cancer Research Center, Zhongshan Hospital Affiliated to Fudan University, and detected by flame atomic absorption spectrophotometer Total iron content in the specimen.

[0048] 2. Establishment of multiple iron deficiency models

[0049] Iron-deficiency ...

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Abstract

The invention relates to the technical field of medicines, in particular to an application of an iron agent in preparation of anti-hepatoma metastasis drugs. The iron agent has the advantages that research finds that hepatoma iron deficiency can significantly promote hepatoma metastasis, in-vivo imaging photographing observation shows that supplementing iron to mice significantly inhibits in-situhepatoma metastasis to lung and significantly inhibits the number and distribution range of lung hepatoma cells, and it is indicated that supplementing iron has an anti-hepatoma lung metastasis effect, and the iron agent has a good clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of iron in the preparation of anti-hepatoma metastasis drugs. Background technique [0002] Liver cancer is a serious health problem worldwide, with more than 850,000 new cases every year, and it is the second deadliest cancer in the world, and the mortality rate is increasing year by year. Liver cancer metastasis is the main cause of death in patients with liver cancer. The mechanism of liver cancer metastasis is still unclear, and there is currently no effective way to prevent the occurrence of metastasis. [0003] Iron is an active factor of iron-containing enzymes / heme-containing enzymes in cells, which affects various physiological and pathological processes of cells. Studies have shown that the iron content of liver cancer tissue in patients with liver cancer is significantly reduced. At present, there are no reports on the relationship between iron defici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/26A61P35/04
CPCA61K9/0019A61K9/0095A61K33/26A61P35/04
Inventor 汤雨潇王冬尧李敏
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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