Droplet dispensing systems
A distribution system and droplet technology, applied in the direction of analysis materials, laboratory containers, instruments, etc., can solve the problems of interfering droplets, droplet entry, etc.
Active Publication Date: 2020-04-17
孚洛飞生物技术有限公司
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Problems solved by technology
In particular, pressure fluctuations associated with dispensing within a microfluidic device may disturb the droplet flow within the device.
Additionally, the dispensing process can sometimes result in droplets or portions of droplets entering undesired microfluidic channels (lines)
Method used
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[0056] Droplet Dispensing System
[0057] refer to figure 1 , which shows a schematic diagram of an example of a droplet dispensing unit with two T-junctions.
[0058] The dispensing unit 10 has a first input channel 15 which supplies microdroplets (droplets with a volume of less than 1 nl). The dispensing unit 10 has a first output channel 40 leading to waste and a second output channel 30 providing an outlet for the dispensing unit 10 for ejecting selected droplets to collect in storage. Droplets are ejected through nozzles 35 . The second input channel 20 is coupled to a distribution valve 25 to receive compressed air in this example. In this way, a pressure pulse can be applied to the emulsion flowing in the second output channel 30 to eject a segment of the emulsion containing selected target droplets for collection in the pores of the porous reservoir. Typically, the pressure pulses are applied by means of a carrier fluid, especially oil, but gas (air) may also be...
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This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outletline; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line toeject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.
Description
technical field [0001] The present invention generally relates to methods and systems for dispensing liquid droplets, particularly microdroplets comprising one or more entities. Background technique [0002] The droplet dispensing techniques described herein typically, but not exclusively, involve emulsions, typically comprising water-in-oil microdroplets, typically surfactant-stabilized. One or more active entities (eg, one or more living cells or particles) can be incorporated into each droplet, and experiments, eg, bioassays, can be performed within the droplets. Microvolume liquid droplets (microdroplets) can be generated and potentially processed at a rate of thousands or more per second. [0003] Typically, the oil composition comprises fluorine and / or mineral oil, and preferably comprises a surfactant, for example about 0.5%-5% v / v. The use of fluoro oils is particularly advantageous when the micro-droplets contain active entities, since fluoro-oils are good at deli...
Claims
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IPC IPC(8): B01L3/00
CPCB01L3/502784B01L2200/0673B01L2400/0487B01L2400/049B01L2400/06B01L2400/0644B01L3/502761B01L2200/0652G01N15/1484B01L3/502715B01L2200/027
Inventor 季米特里斯·约瑟费迪斯弗兰克·F·克雷格玛丽安·雷哈克大卫·霍姆斯
Owner 孚洛飞生物技术有限公司